MRNC_BACCR
ID MRNC_BACCR Reviewed; 135 AA.
AC Q81J58;
DT 21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 100.
DE RecName: Full=Mini-ribonuclease 3 {ECO:0000255|HAMAP-Rule:MF_01468};
DE Short=Mini-3 {ECO:0000255|HAMAP-Rule:MF_01468};
DE Short=Mini-RNase 3 {ECO:0000255|HAMAP-Rule:MF_01468};
DE EC=3.1.26.- {ECO:0000255|HAMAP-Rule:MF_01468};
DE AltName: Full=Mini-RNase III {ECO:0000255|HAMAP-Rule:MF_01468};
DE Short=Mini-III {ECO:0000255|HAMAP-Rule:MF_01468};
GN Name=mrnC {ECO:0000255|HAMAP-Rule:MF_01468}; OrderedLocusNames=BC_0111;
OS Bacillus cereus (strain ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC
OS 15305 / NCIMB 9373 / NCTC 2599 / NRRL B-3711).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=226900;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373
RC / NCTC 2599 / NRRL B-3711;
RX PubMed=12721630; DOI=10.1038/nature01582;
RA Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V.,
RA Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M.,
RA Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G.,
RA Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C.;
RT "Genome sequence of Bacillus cereus and comparative analysis with Bacillus
RT anthracis.";
RL Nature 423:87-91(2003).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS).
RC STRAIN=ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373
RC / NCTC 2599 / NRRL B-3711;
RA Osipiuk J., Quartey P., Moy S., Collart F., Joachimiak A.;
RT "X-ray crystal structure of conserved hypothetical protein from Bacillus
RT cereus.";
RL Submitted (JUL-2004) to the PDB data bank.
CC -!- FUNCTION: Involved in correct processing of both the 5' and 3' ends of
CC 23S rRNA precursor. Processes 30S rRNA precursor transcript even in
CC absence of ribonuclease 3 (Rnc); Rnc processes 30S rRNA into smaller
CC rRNA precursors. {ECO:0000255|HAMAP-Rule:MF_01468}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01468};
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01468}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01468}.
CC -!- SIMILARITY: Belongs to the MrnC RNase family. {ECO:0000255|HAMAP-
CC Rule:MF_01468}.
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DR EMBL; AE016877; AAP07193.1; -; Genomic_DNA.
DR RefSeq; NP_829992.1; NC_004722.1.
DR RefSeq; WP_000564262.1; NZ_CP034551.1.
DR PDB; 1U61; X-ray; 2.15 A; A=1-135.
DR PDBsum; 1U61; -.
DR AlphaFoldDB; Q81J58; -.
DR SMR; Q81J58; -.
DR STRING; 226900.BC_0111; -.
DR EnsemblBacteria; AAP07193; AAP07193; BC_0111.
DR KEGG; bce:BC0111; -.
DR PATRIC; fig|226900.8.peg.113; -.
DR HOGENOM; CLU_091169_2_0_9; -.
DR OMA; AYMGDAI; -.
DR EvolutionaryTrace; Q81J58; -.
DR Proteomes; UP000001417; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004525; F:ribonuclease III activity; IEA:InterPro.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-UniRule.
DR CDD; cd00593; RIBOc; 1.
DR Gene3D; 1.10.1520.10; -; 1.
DR HAMAP; MF_01468; RNase_Mini_III; 1.
DR InterPro; IPR008226; Mini3_fam.
DR InterPro; IPR000999; RNase_III_dom.
DR InterPro; IPR036389; RNase_III_sf.
DR Pfam; PF00636; Ribonuclease_3; 1.
DR PIRSF; PIRSF005520; UCP005520; 1.
DR SMART; SM00535; RIBOc; 1.
DR SUPFAM; SSF69065; SSF69065; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Endonuclease; Hydrolase; Magnesium; Nuclease;
KW Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing;
KW rRNA-binding.
FT CHAIN 1..135
FT /note="Mini-ribonuclease 3"
FT /id="PRO_0000415980"
FT ACT_SITE 17
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01468"
FT TURN 4..6
FT /evidence="ECO:0007829|PDB:1U61"
FT HELIX 9..31
FT /evidence="ECO:0007829|PDB:1U61"
FT HELIX 36..38
FT /evidence="ECO:0007829|PDB:1U61"
FT HELIX 39..47
FT /evidence="ECO:0007829|PDB:1U61"
FT HELIX 49..61
FT /evidence="ECO:0007829|PDB:1U61"
FT HELIX 67..76
FT /evidence="ECO:0007829|PDB:1U61"
FT HELIX 90..108
FT /evidence="ECO:0007829|PDB:1U61"
FT HELIX 112..127
FT /evidence="ECO:0007829|PDB:1U61"
SQ SEQUENCE 135 AA; 15290 MW; 9B313191657E663B CRC64;
MIDAKQLNSL ALAYMGDAVY EQYIRYHLLQ KGKVRPNQLH RLGTSFVSAK AQAKVVYHLL
ETAFLTEEEE AVLRRGRNAN SGTVPKNTDV QTYRHSTAFE ALIGYHHLLN NRERLDEIVY
KAIAVLEEQE GGTSS
