ID   MRNC_CLOTE              Reviewed;         151 AA.
AC   Q890M5;
DT   21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Mini-ribonuclease 3 {ECO:0000255|HAMAP-Rule:MF_01468};
DE            Short=Mini-3 {ECO:0000255|HAMAP-Rule:MF_01468};
DE            Short=Mini-RNase 3 {ECO:0000255|HAMAP-Rule:MF_01468};
DE            EC=3.1.26.- {ECO:0000255|HAMAP-Rule:MF_01468};
DE   AltName: Full=Mini-RNase III {ECO:0000255|HAMAP-Rule:MF_01468};
DE            Short=Mini-III {ECO:0000255|HAMAP-Rule:MF_01468};
GN   Name=mrnC {ECO:0000255|HAMAP-Rule:MF_01468}; OrderedLocusNames=CTC_02621;
OS   Clostridium tetani (strain Massachusetts / E88).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=212717;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Massachusetts / E88;
RX   PubMed=12552129; DOI=10.1073/pnas.0335853100;
RA   Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H.,
RA   Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A.,
RA   Gottschalk G.;
RT   "The genome sequence of Clostridium tetani, the causative agent of tetanus
RT   disease.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003).
CC   -!- FUNCTION: Involved in correct processing of both the 5' and 3' ends of
CC       23S rRNA precursor. Processes 30S rRNA precursor transcript even in
CC       absence of ribonuclease 3 (Rnc); Rnc processes 30S rRNA into smaller
CC       rRNA precursors. {ECO:0000255|HAMAP-Rule:MF_01468}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01468};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01468}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01468}.
CC   -!- SIMILARITY: Belongs to the MrnC RNase family. {ECO:0000255|HAMAP-
CC       Rule:MF_01468}.
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DR   EMBL; AE015927; AAO37072.1; -; Genomic_DNA.
DR   RefSeq; WP_011100732.1; NC_004557.1.
DR   AlphaFoldDB; Q890M5; -.
DR   SMR; Q890M5; -.
DR   STRING; 212717.CTC_02621; -.
DR   EnsemblBacteria; AAO37072; AAO37072; CTC_02621.
DR   GeneID; 64179919; -.
DR   KEGG; ctc:CTC_02621; -.
DR   HOGENOM; CLU_091169_2_1_9; -.
DR   OMA; AYMGDAI; -.
DR   OrthoDB; 1939897at2; -.
DR   Proteomes; UP000001412; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004525; F:ribonuclease III activity; IEA:InterPro.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-UniRule.
DR   CDD; cd00593; RIBOc; 1.
DR   Gene3D; 1.10.1520.10; -; 1.
DR   HAMAP; MF_01468; RNase_Mini_III; 1.
DR   InterPro; IPR008226; Mini3_fam.
DR   InterPro; IPR000999; RNase_III_dom.
DR   InterPro; IPR036389; RNase_III_sf.
DR   Pfam; PF00636; Ribonuclease_3; 1.
DR   PIRSF; PIRSF005520; UCP005520; 1.
DR   SUPFAM; SSF69065; SSF69065; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Endonuclease; Hydrolase; Magnesium; Nuclease;
KW   Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing;
KW   rRNA-binding.
FT   CHAIN           1..151
FT                   /note="Mini-ribonuclease 3"
FT                   /id="PRO_0000415982"
FT   ACT_SITE        28
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01468"
SQ   SEQUENCE   151 AA;  17744 MW;  E65D46564F96F8D1 CRC64;
     MRKYFLGEEF TKQDLKQMNP LVLAYIGDAV YEIFIRTYII SKNKDTCVNK LHKKTIQFVK
     ADAQSYFIRE IQKYLNEEEI KVFKRGRNTK SNTSAKNASI QDYRMATGFE TVIGYLYLLN
     EDERLEEIMK LVINLYEESS QNEGENHGSK S