ID   MRNC_KOSOT              Reviewed;         145 AA.
AC   C5CF30;
DT   21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Mini-ribonuclease 3 {ECO:0000255|HAMAP-Rule:MF_01468};
DE            Short=Mini-3 {ECO:0000255|HAMAP-Rule:MF_01468};
DE            Short=Mini-RNase 3 {ECO:0000255|HAMAP-Rule:MF_01468};
DE            EC=3.1.26.- {ECO:0000255|HAMAP-Rule:MF_01468};
DE   AltName: Full=Mini-RNase III {ECO:0000255|HAMAP-Rule:MF_01468};
DE            Short=Mini-III {ECO:0000255|HAMAP-Rule:MF_01468};
GN   Name=mrnC {ECO:0000255|HAMAP-Rule:MF_01468}; OrderedLocusNames=Kole_1605;
OS   Kosmotoga olearia (strain ATCC BAA-1733 / DSM 21960 / TBF 19.5.1).
OC   Bacteria; Thermotogae; Kosmotogales; Kosmotogaceae; Kosmotoga.
OX   NCBI_TaxID=521045;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1733 / DSM 21960 / TBF 19.5.1;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D.,
RA   Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C.,
RA   Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.,
RA   Noll K.;
RT   "Complete sequence of Thermotogales bacterium TBF 19.5.1.";
RL   Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in correct processing of both the 5' and 3' ends of
CC       23S rRNA precursor. Processes 30S rRNA precursor transcript even in
CC       absence of ribonuclease 3 (Rnc); Rnc processes 30S rRNA into smaller
CC       rRNA precursors. {ECO:0000255|HAMAP-Rule:MF_01468}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01468};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01468}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01468}.
CC   -!- SIMILARITY: Belongs to the MrnC RNase family. {ECO:0000255|HAMAP-
CC       Rule:MF_01468}.
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DR   EMBL; CP001634; ACR80295.1; -; Genomic_DNA.
DR   RefSeq; WP_015868940.1; NC_012785.1.
DR   AlphaFoldDB; C5CF30; -.
DR   SMR; C5CF30; -.
DR   STRING; 521045.Kole_1605; -.
DR   PRIDE; C5CF30; -.
DR   EnsemblBacteria; ACR80295; ACR80295; Kole_1605.
DR   KEGG; kol:Kole_1605; -.
DR   eggNOG; COG1939; Bacteria.
DR   HOGENOM; CLU_091169_2_1_0; -.
DR   OMA; AYMGDAI; -.
DR   OrthoDB; 1939897at2; -.
DR   Proteomes; UP000002382; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004525; F:ribonuclease III activity; IEA:InterPro.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.1520.10; -; 1.
DR   HAMAP; MF_01468; RNase_Mini_III; 1.
DR   InterPro; IPR008226; Mini3_fam.
DR   InterPro; IPR000999; RNase_III_dom.
DR   InterPro; IPR036389; RNase_III_sf.
DR   Pfam; PF00636; Ribonuclease_3; 1.
DR   PIRSF; PIRSF005520; UCP005520; 1.
DR   SUPFAM; SSF69065; SSF69065; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Endonuclease; Hydrolase; Magnesium; Nuclease;
KW   Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing;
KW   rRNA-binding.
FT   CHAIN           1..145
FT                   /note="Mini-ribonuclease 3"
FT                   /id="PRO_0000415986"
FT   ACT_SITE        27
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01468"
SQ   SEQUENCE   145 AA;  17004 MW;  99035BD4C0B484E1 CRC64;
     MSDFENFFRT DIVPEELSID SLSFVGDAVY SLYFRIKTLR SAKRRTGYQH NLTLLYVEAK
     GQRKALEVIE KFLSEEERII VRRGYNSRGA RKRGDDENYK CATALEALVG YLFLKGRYRR
     LEELLEKVEN DVSSWKKCAE RDSKT