ID   MRNC_STRR6              Reviewed;         128 AA.
AC   Q8DQS7;
DT   21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Mini-ribonuclease 3 {ECO:0000255|HAMAP-Rule:MF_01468};
DE            Short=Mini-3 {ECO:0000255|HAMAP-Rule:MF_01468};
DE            Short=Mini-RNase 3 {ECO:0000255|HAMAP-Rule:MF_01468};
DE            EC=3.1.26.- {ECO:0000255|HAMAP-Rule:MF_01468};
DE   AltName: Full=Mini-RNase III {ECO:0000255|HAMAP-Rule:MF_01468};
DE            Short=Mini-III {ECO:0000255|HAMAP-Rule:MF_01468};
GN   Name=mrnC {ECO:0000255|HAMAP-Rule:MF_01468}; OrderedLocusNames=spr0520;
OS   Streptococcus pneumoniae (strain ATCC BAA-255 / R6).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=171101;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-255 / R6;
RX   PubMed=11544234; DOI=10.1128/jb.183.19.5709-5717.2001;
RA   Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S.,
RA   DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C.,
RA   Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J.,
RA   Lee L.N., Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M.,
RA   McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B.,
RA   Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y.,
RA   Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R.,
RA   Rosteck P.R. Jr., Skatrud P.L., Glass J.I.;
RT   "Genome of the bacterium Streptococcus pneumoniae strain R6.";
RL   J. Bacteriol. 183:5709-5717(2001).
CC   -!- FUNCTION: Involved in correct processing of both the 5' and 3' ends of
CC       23S rRNA precursor. Processes 30S rRNA precursor transcript even in
CC       absence of ribonuclease 3 (Rnc); Rnc processes 30S rRNA into smaller
CC       rRNA precursors. {ECO:0000255|HAMAP-Rule:MF_01468}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01468};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01468}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01468}.
CC   -!- SIMILARITY: Belongs to the MrnC RNase family. {ECO:0000255|HAMAP-
CC       Rule:MF_01468}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE007317; AAK99324.1; -; Genomic_DNA.
DR   PIR; H95068; H95068.
DR   PIR; H97936; H97936.
DR   RefSeq; NP_358114.1; NC_003098.1.
DR   RefSeq; WP_000567914.1; NC_003098.1.
DR   AlphaFoldDB; Q8DQS7; -.
DR   SMR; Q8DQS7; -.
DR   STRING; 171101.spr0520; -.
DR   EnsemblBacteria; AAK99324; AAK99324; spr0520.
DR   GeneID; 60233565; -.
DR   GeneID; 66805753; -.
DR   KEGG; spr:spr0520; -.
DR   PATRIC; fig|171101.6.peg.572; -.
DR   eggNOG; COG1939; Bacteria.
DR   HOGENOM; CLU_091169_2_0_9; -.
DR   OMA; AYMGDAI; -.
DR   Proteomes; UP000000586; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004525; F:ribonuclease III activity; IEA:InterPro.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-UniRule.
DR   CDD; cd00593; RIBOc; 1.
DR   Gene3D; 1.10.1520.10; -; 1.
DR   HAMAP; MF_01468; RNase_Mini_III; 1.
DR   InterPro; IPR008226; Mini3_fam.
DR   InterPro; IPR000999; RNase_III_dom.
DR   InterPro; IPR036389; RNase_III_sf.
DR   Pfam; PF00636; Ribonuclease_3; 1.
DR   PIRSF; PIRSF005520; UCP005520; 1.
DR   SUPFAM; SSF69065; SSF69065; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Endonuclease; Hydrolase; Magnesium; Nuclease;
KW   Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing;
KW   rRNA-binding.
FT   CHAIN           1..128
FT                   /note="Mini-ribonuclease 3"
FT                   /id="PRO_0000415992"
FT   ACT_SITE        17
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01468"
SQ   SEQUENCE   128 AA;  14636 MW;  F80D62FE3F018626 CRC64;
     MIDVNLINGI ALAFEGDAVY SMYIRRHLIL KGMTKPNKLH QEATKYVSAK AQARLIALML
     EEQVLTEKEE EIYKRGRNTN SHTKAKNADV VTYRMSTGFE AVMGYLHMTE NLERLESLVS
     WCIQKVEG