ID   MRNC_THEPX              Reviewed;         142 AA.
AC   B0K5F7;
DT   21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2012, sequence version 2.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=Mini-ribonuclease 3 {ECO:0000255|HAMAP-Rule:MF_01468};
DE            Short=Mini-3 {ECO:0000255|HAMAP-Rule:MF_01468};
DE            Short=Mini-RNase 3 {ECO:0000255|HAMAP-Rule:MF_01468};
DE            EC=3.1.26.- {ECO:0000255|HAMAP-Rule:MF_01468};
DE   AltName: Full=Mini-RNase III {ECO:0000255|HAMAP-Rule:MF_01468};
DE            Short=Mini-III {ECO:0000255|HAMAP-Rule:MF_01468};
GN   Name=mrnC {ECO:0000255|HAMAP-Rule:MF_01468};
GN   OrderedLocusNames=Teth514_0847;
OS   Thermoanaerobacter sp. (strain X514).
OC   Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC   Thermoanaerobacteraceae; Thermoanaerobacter;
OC   unclassified Thermoanaerobacter.
OX   NCBI_TaxID=399726;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=X514;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Bruce D., Goodwin L., Saunders E., Brettin T.,
RA   Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L.,
RA   Kyrpides N., Kim E., Hemme C., Fields M.W., He Z., Zhou J., Richardson P.;
RT   "Complete sequence of Thermoanaerobacter sp. X514.";
RL   Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in correct processing of both the 5' and 3' ends of
CC       23S rRNA precursor. Processes 30S rRNA precursor transcript even in
CC       absence of ribonuclease 3 (Rnc); Rnc processes 30S rRNA into smaller
CC       rRNA precursors. {ECO:0000255|HAMAP-Rule:MF_01468}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01468};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01468}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01468}.
CC   -!- SIMILARITY: Belongs to the MrnC RNase family. {ECO:0000255|HAMAP-
CC       Rule:MF_01468}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABY92150.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; CP000923; ABY92150.1; ALT_FRAME; Genomic_DNA.
DR   AlphaFoldDB; B0K5F7; -.
DR   SMR; B0K5F7; -.
DR   EnsemblBacteria; ABY92150; ABY92150; Teth514_0847.
DR   KEGG; tex:Teth514_0847; -.
DR   HOGENOM; CLU_091169_2_1_9; -.
DR   Proteomes; UP000002155; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004525; F:ribonuclease III activity; IEA:InterPro.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-UniRule.
DR   CDD; cd00593; RIBOc; 1.
DR   Gene3D; 1.10.1520.10; -; 1.
DR   HAMAP; MF_01468; RNase_Mini_III; 1.
DR   InterPro; IPR008226; Mini3_fam.
DR   InterPro; IPR000999; RNase_III_dom.
DR   InterPro; IPR036389; RNase_III_sf.
DR   Pfam; PF00636; Ribonuclease_3; 1.
DR   PIRSF; PIRSF005520; UCP005520; 1.
DR   SUPFAM; SSF69065; SSF69065; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Endonuclease; Hydrolase; Magnesium; Nuclease;
KW   Ribosome biogenesis; RNA-binding; rRNA processing; rRNA-binding.
FT   CHAIN           1..142
FT                   /note="Mini-ribonuclease 3"
FT                   /id="PRO_0000415997"
FT   ACT_SITE        33
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01468"
SQ   SEQUENCE   142 AA;  16198 MW;  B8112844BAEAA321 CRC64;
     MEKSMMGFLE NTELLTKEGV LSLSPLVLAF IGDAVYSLYI RTKIVAQKNQ PVNFLHKETV
     KYVKAKAQAE SVKRIYDLLS EEEKDIVRRG RNMKSNTTPK GVEVQAYRYA TGFEALLGYL
     YLAGEFERLK NILELSVQVI EE