MRO2B_MOUSE
ID MRO2B_MOUSE Reviewed; 1581 AA.
AC Q7M6Y6; A0A096XFP7; A0A096XFP8;
DT 15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT 15-JUN-2010, sequence version 2.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Maestro heat-like repeat-containing protein family member 2B {ECO:0000312|MGI:MGI:1921905};
DE AltName: Full=HEAT repeat-containing protein 7B2;
DE AltName: Full=Sperm PKA-interacting factor {ECO:0000303|PubMed:27105888};
DE Short=SPIF {ECO:0000303|PubMed:27105888};
GN Name=Mroh2b {ECO:0000312|MGI:MGI:1921905}; Synonyms=Heatr7b2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), IDENTIFICATION IN A COMPLEX
RP WITH MROH2B; PRKACA AND TCP11, INTERACTION WITH PRKACA AND TCP11,
RP SUBCELLULAR LOCATION, POSSIBLE FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE
RP SPECIFICITY.
RX PubMed=27105888; DOI=10.1096/fj.201500136r;
RA Stanger S.J., Law E.A., Jamsai D., O'Bryan M.K., Nixon B., McLaughlin E.A.,
RA Aitken R.J., Roman S.D.;
RT "A novel germ cell protein, SPIF (sperm PKA interacting factor), is
RT essential for the formation of a PKA/TCP11 complex that undergoes
RT conformational and phosphorylation changes upon capacitation.";
RL FASEB J. 30:2777-2791(2016).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 959-1581 (ISOFORMS 1/2).
RC STRAIN=C57BL/6J;
RX PubMed=12783626; DOI=10.1186/1471-2164-4-22;
RA Oduru S., Campbell J.L., Karri S., Hendry W.J., Khan S.A., Williams S.C.;
RT "Gene discovery in the hamster: a comparative genomics approach for gene
RT annotation by sequencing of hamster testis cDNAs.";
RL BMC Genomics 4:22-22(2003).
CC -!- FUNCTION: May play a role in the process of sperm capacitation
CC (PubMed:27105888). {ECO:0000269|PubMed:27105888}.
CC -!- SUBUNIT: Found in a complex at least composed of MROH2B isoform 2,
CC PRKACA isoform 2 and TCP11 (PubMed:27105888). Interacts with PRKACA
CC isoform 2 (PubMed:27105888). Interacts with TCP11 (PubMed:27105888).
CC {ECO:0000269|PubMed:27105888}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:27105888}.
CC Cytoplasmic vesicle, secretory vesicle, acrosome
CC {ECO:0000269|PubMed:27105888}. Cell projection, cilium, flagellum
CC {ECO:0000269|PubMed:27105888}. Note=Colocalizes with PRKACA and TCP11
CC on the acrosome and tail regions in round spermatids and spermatozoa
CC regardless of the capacitation status of the sperm.
CC {ECO:0000269|PubMed:27105888}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q7M6Y6-1; Sequence=Displayed;
CC Name=2; Synonyms=SPIF-L {ECO:0000303|PubMed:27105888};
CC IsoId=Q7M6Y6-2; Sequence=VSP_058722;
CC Name=3; Synonyms=SPIF-S {ECO:0000303|PubMed:27105888};
CC IsoId=Q7M6Y6-3; Sequence=VSP_058721;
CC -!- TISSUE SPECIFICITY: Expressed strongly in round spermatids and fully
CC mature spermatozoa. Expressed weakly in pachytene spermatocytes (at
CC protein level). Isoform 2 is specifically expressed in the testis.
CC Isoform 2 is expressed in pachytene spermatocytes and round spermatids.
CC Isoform 3 is weakly expressed in testis. {ECO:0000269|PubMed:27105888}.
CC -!- PTM: Constitutively phosphorylated on serine and threonine residues in
CC acrosomal region of the sperm head, midpiece and flagellar regions of
CC noncapacitated spermatozoa. Phosphorylation on tyrosine residues
CC increases upon sperm capacitation within the acrosomal and tail regions
CC in a protein kinase A (PKA)-dependent signaling pathway.
CC {ECO:0000269|PubMed:27105888}.
CC -!- DISRUPTION PHENOTYPE: Embryonically lethal before 10 dpc.
CC {ECO:0000269|PubMed:27105888}.
CC -!- SEQUENCE CAUTION:
CC Sequence=DAA01465.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; KF778378; AHK25002.1; -; mRNA.
DR EMBL; KF778379; AHK25003.1; -; mRNA.
DR EMBL; AC115877; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC117588; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BK001331; DAA01465.1; ALT_INIT; mRNA.
DR CCDS; CCDS49572.1; -. [Q7M6Y6-1]
DR RefSeq; NP_001159538.1; NM_001166066.1. [Q7M6Y6-1]
DR AlphaFoldDB; Q7M6Y6; -.
DR IntAct; Q7M6Y6; 1.
DR MINT; Q7M6Y6; -.
DR STRING; 10090.ENSMUSP00000036148; -.
DR iPTMnet; Q7M6Y6; -.
DR PhosphoSitePlus; Q7M6Y6; -.
DR EPD; Q7M6Y6; -.
DR PaxDb; Q7M6Y6; -.
DR PRIDE; Q7M6Y6; -.
DR ProteomicsDB; 290320; -. [Q7M6Y6-1]
DR ProteomicsDB; 290321; -. [Q7M6Y6-2]
DR ProteomicsDB; 290322; -. [Q7M6Y6-3]
DR Antibodypedia; 56251; 19 antibodies from 6 providers.
DR Ensembl; ENSMUST00000045736; ENSMUSP00000036148; ENSMUSG00000022155. [Q7M6Y6-1]
DR GeneID; 223825; -.
DR KEGG; mmu:223825; -.
DR UCSC; uc011zqx.1; mouse. [Q7M6Y6-1]
DR CTD; 133558; -.
DR MGI; MGI:1921905; Mroh2b.
DR VEuPathDB; HostDB:ENSMUSG00000022155; -.
DR eggNOG; KOG2032; Eukaryota.
DR GeneTree; ENSGT00940000161231; -.
DR HOGENOM; CLU_003168_1_0_1; -.
DR InParanoid; Q7M6Y6; -.
DR OMA; HREDFCE; -.
DR OrthoDB; 31329at2759; -.
DR PhylomeDB; Q7M6Y6; -.
DR TreeFam; TF315201; -.
DR BioGRID-ORCS; 223825; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Mroh2b; mouse.
DR PRO; PR:Q7M6Y6; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q7M6Y6; protein.
DR Bgee; ENSMUSG00000022155; Expressed in spermatocyte and 6 other tissues.
DR Genevisible; Q7M6Y6; MM.
DR GO; GO:0001669; C:acrosomal vesicle; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0036126; C:sperm flagellum; IDA:UniProtKB.
DR GO; GO:0097225; C:sperm midpiece; IDA:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0010737; P:protein kinase A signaling; IDA:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR Gene3D; 1.25.10.10; -; 2.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR045206; Maestro_heat-like_prot.
DR PANTHER; PTHR23120; PTHR23120; 1.
DR SUPFAM; SSF48371; SSF48371; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cilium; Cytoplasm;
KW Cytoplasmic vesicle; Differentiation; Flagellum; Reference proteome;
KW Repeat; Spermatogenesis.
FT CHAIN 1..1581
FT /note="Maestro heat-like repeat-containing protein family
FT member 2B"
FT /id="PRO_0000395105"
FT REPEAT 123..160
FT /note="HEAT 1"
FT REPEAT 305..342
FT /note="HEAT 2"
FT REPEAT 401..441
FT /note="HEAT 3"
FT REPEAT 464..501
FT /note="HEAT 4"
FT REPEAT 526..543
FT /note="HEAT 5"
FT REPEAT 544..580
FT /note="HEAT 6"
FT REPEAT 658..695
FT /note="HEAT 7"
FT REPEAT 773..815
FT /note="HEAT 8"
FT REPEAT 960..997
FT /note="HEAT 9"
FT REPEAT 1017..1055
FT /note="HEAT 10"
FT REPEAT 1112..1150
FT /note="HEAT 11"
FT REPEAT 1153..1191
FT /note="HEAT 12"
FT REPEAT 1254..1291
FT /note="HEAT 13"
FT REPEAT 1295..1332
FT /note="HEAT 14"
FT REPEAT 1359..1379
FT /note="HEAT 15"
FT REPEAT 1380..1416
FT /note="HEAT 16"
FT VAR_SEQ 1..743
FT /note="MEEYGDMFGDINLTIGMLSKEDNISKEDIYCHLTSFIQNTDIMDDAIVQRLI
FT YYTSKDMRDEEIPRELRMLAGEVLVSLAAHDFNSVMYEVQSNFRILELPNEFVVLALAE
FT LATSYVSQSIPFMMMTLLTMQTMLRLVEDENMRQTFCIALENFSKSIYKYVNHWKDFPY
FT PKLDANRLSDKIFMLFRYIMEKWAPQASPMHALAIIKAHGPTVSLLLHREDFCEFALSQ
FT ISWLLLQYRDKENDFYITQSLKQILTAAVLYDIALPKNLRRSVLSSLLHQICKVPEPPI
FT KENKLEASSCFLILAHANPVDLLDFFDEQIRSTNEAVRTGILTLLRSTINAEEPKFRNH
FT TTSIEKTVKLVMGDLSVKVRKSTLLLIQTMCEKGYIEAREGWPLIDYIFSQFAMSNRNL
FT ENPIKSNSQEDENGEKSVQETSLEVLKSLDPLVIGMPQVLWPRILTYVVPKEYTGTLDY
FT LFNIIRILIMAEEKKKRDIQESTALVVSTGAVKLPSPQQLLARLLVISILASLGQLCGA
FT GAIGLLKIMPEIIHPKLAEMWKTRMPALLQPLEGSNASIVLWETMLLQLLKESLWKISD
FT VAWTSQLSRDFSLQMGSYSNSSMEKKFLWKALGTTLASCQDKDFVSSQINEFLVTPSLL
FT GDHRQGTTSILGFCAENHLDIVLNVLKTFQDKEKFFVNRCKGIFSGKKSLTKTDLILIY
FT GAVALHAPKQQLLARLDQDIMGQILLLYGQCCQILGVSVINK -> MAHAKSIPNASVP
FT QEQIQTLQHKLDYGSDCSFHFIALPTIYSGNIITFLPAKCRAFFEPKNPSLPFLLLLFV
FT FYLP (in isoform 3)"
FT /id="VSP_058721"
FT VAR_SEQ 739..743
FT /note="Missing (in isoform 2)"
FT /id="VSP_058722"
FT CONFLICT 401
FT /note="M -> V (in Ref. 1; AHK25002)"
FT /evidence="ECO:0000305"
FT CONFLICT 659
FT /note="N -> S (in Ref. 1; AHK25002)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1581 AA; 180480 MW; 0314A4B1659ACF95 CRC64;
MEEYGDMFGD INLTIGMLSK EDNISKEDIY CHLTSFIQNT DIMDDAIVQR LIYYTSKDMR
DEEIPRELRM LAGEVLVSLA AHDFNSVMYE VQSNFRILEL PNEFVVLALA ELATSYVSQS
IPFMMMTLLT MQTMLRLVED ENMRQTFCIA LENFSKSIYK YVNHWKDFPY PKLDANRLSD
KIFMLFRYIM EKWAPQASPM HALAIIKAHG PTVSLLLHRE DFCEFALSQI SWLLLQYRDK
ENDFYITQSL KQILTAAVLY DIALPKNLRR SVLSSLLHQI CKVPEPPIKE NKLEASSCFL
ILAHANPVDL LDFFDEQIRS TNEAVRTGIL TLLRSTINAE EPKFRNHTTS IEKTVKLVMG
DLSVKVRKST LLLIQTMCEK GYIEAREGWP LIDYIFSQFA MSNRNLENPI KSNSQEDENG
EKSVQETSLE VLKSLDPLVI GMPQVLWPRI LTYVVPKEYT GTLDYLFNII RILIMAEEKK
KRDIQESTAL VVSTGAVKLP SPQQLLARLL VISILASLGQ LCGAGAIGLL KIMPEIIHPK
LAEMWKTRMP ALLQPLEGSN ASIVLWETML LQLLKESLWK ISDVAWTSQL SRDFSLQMGS
YSNSSMEKKF LWKALGTTLA SCQDKDFVSS QINEFLVTPS LLGDHRQGTT SILGFCAENH
LDIVLNVLKT FQDKEKFFVN RCKGIFSGKK SLTKTDLILI YGAVALHAPK QQLLARLDQD
IMGQILLLYG QCCQILGVSV INKDMDLQMS FTRSITEVGI AVQDAEDQKF QFTYKEMLIG
SMLDLIKDEP LNTLASPVRW KVLIAIRYLS KLKPALSLND HLNILEENIR RLLPLPPLEK
LKSQGETDKD RERIEFLYER SMDALGKLLR SMIWDNTDAQ NCEEMFNLLR MWLVSQKQWE
RERAFQVTSK VLTKDVEAPQ NFRIGSLLGL LAPHSCDTLP TIRQAATSST IGLLCAKGIC
QEVDRLQGLQ EGLDSEDEQV QIKISSKIAK IVCKFIPSEE IQVFLEETLD GLETLDPLCT
KACGIWMIAA LKEHGALLED QLLEILSTIY HHMPVLRQKE ESFQFMLEAI SQIASFHMDA
VVNNLLQKPL PFDRDTKTLW KALAENPASS GKLMRALIKK LVARLEDDIA GTEAISVACA
IYEVILTGAH ITHLYPELFT LLLKLVSCSL GQKMPMSTLS QRRRVMQLGE RQRFPDPCRL
STATLKCLQA QAMREGLAKE SDEGDNLWTL LSNPDTHHIG VCALARSMAV WQHGVILDIM
EHLLSSLTSS SENYRITGMA FFSELMKEPI LWKHGNLRDV LIFMDQNARD SNAILRQMAI
RGLGNTACGA PHKVRKYKQM MLECIIRGLY HLARTEVVCE SLKALKKILE LLTERDINFY
FKEIVLQTRT FFEDEQDDVR LTAISLFEDL ATLTGRRWKI FFAEEVKKSM ISFLLHLWDP
NPKIGAACRD VLVICIPFLG LQELYGLLDH LLERDLPRAR DFYRQLCMKL SKKNQEILWI
LHTHSFTFFT SSWEMIRSAA VKLTDAIILH LTKRYVELLD REQLTMRLQA LRQDPCISVQ
RAAEATLQTL LRRCKEISIP L
