MRP2A_DANRE
ID MRP2A_DANRE Reviewed; 217 AA.
AC F8W4H9;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 21-SEP-2011, sequence version 1.
DT 03-AUG-2022, entry version 46.
DE RecName: Full=Melanocortin-2 receptor accessory protein 2A;
DE Short=zMRAP2a;
GN Name=mrap2a;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP FUNCTION, INTERACTION WITH MC4R, AND DEVELOPMENTAL STAGE.
RX PubMed=23869017; DOI=10.1126/science.1232995;
RA Sebag J.A., Zhang C., Hinkle P.M., Bradshaw A.M., Cone R.D.;
RT "Developmental control of the melanocortin-4 receptor by MRAP2 proteins in
RT zebrafish.";
RL Science 341:278-281(2013).
CC -!- FUNCTION: Inhibitor of melanocortin receptor 4 (mc4r), a receptor
CC involved in energy homeostasis. Plays a role during larval development
CC in the control of energy homeostasis and body weight regulation by
CC decreasing ligand-sensitivity of mc4r and mc4r-mediated generation of
CC cAMP, leading to stimulate growth during larval development. Acts by
CC stabilizing an inactive conformation of mc4r during embryonic
CC development, when all the energy consumed is obtained from the yolk
CC sac, possibly to speed the rapid maturation to the mobile free-feeding
CC juvenile stage reached at 5 dpf. {ECO:0000269|PubMed:23869017}.
CC -!- SUBUNIT: Interacts with mc4r. {ECO:0000269|PubMed:23869017}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass membrane
CC protein {ECO:0000250}. Endoplasmic reticulum membrane {ECO:0000250};
CC Single-pass membrane protein {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Expressed from 1 post-fertilization (dpf) and
CC expression increases until 4 dpf. Ubiquitously expressed at 5 dpf.
CC {ECO:0000269|PubMed:23869017}.
CC -!- MISCELLANEOUS: Two paralogs of MRAP2 are present in zebrafish: mrap2a,
CC which plays a role during larval development and mrap2b, which
CC constitutes the ortholog of mammalian MRAP2.
CC {ECO:0000305|PubMed:23869017}.
CC -!- SIMILARITY: Belongs to the MRAP family. {ECO:0000305}.
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DR EMBL; BX088579; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_001342923.4; XM_001342887.6.
DR AlphaFoldDB; F8W4H9; -.
DR SMR; F8W4H9; -.
DR STRING; 7955.ENSDARP00000124181; -.
DR PaxDb; F8W4H9; -.
DR Ensembl; ENSDART00000148801; ENSDARP00000124181; ENSDARG00000074763.
DR GeneID; 100003330; -.
DR KEGG; dre:100003330; -.
DR CTD; 100003330; -.
DR ZFIN; ZDB-GENE-100729-3; mrap2a.
DR eggNOG; ENOG502RYQM; Eukaryota.
DR GeneTree; ENSGT00650000093438; -.
DR HOGENOM; CLU_110753_0_0_1; -.
DR InParanoid; F8W4H9; -.
DR OMA; WDYEYYE; -.
DR OrthoDB; 1318662at2759; -.
DR TreeFam; TF338691; -.
DR PRO; PR:F8W4H9; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 16.
DR Bgee; ENSDARG00000074763; Expressed in brain and 12 other tissues.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:ZFIN.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; IDA:ZFIN.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:ZFIN.
DR GO; GO:0031780; F:corticotropin hormone receptor binding; IBA:GO_Central.
DR GO; GO:0030545; F:signaling receptor regulator activity; IBA:GO_Central.
DR GO; GO:0070996; F:type 1 melanocortin receptor binding; IBA:GO_Central.
DR GO; GO:0031781; F:type 3 melanocortin receptor binding; IBA:GO_Central.
DR GO; GO:0031782; F:type 4 melanocortin receptor binding; IDA:UniProtKB.
DR GO; GO:0031783; F:type 5 melanocortin receptor binding; IBA:GO_Central.
DR GO; GO:0048589; P:developmental growth; IMP:ZFIN.
DR GO; GO:0097009; P:energy homeostasis; IMP:UniProtKB.
DR GO; GO:0006112; P:energy reserve metabolic process; IMP:UniProtKB.
DR GO; GO:0106072; P:negative regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway; IMP:ZFIN.
DR GO; GO:0034394; P:protein localization to cell surface; IDA:ZFIN.
DR GO; GO:0072659; P:protein localization to plasma membrane; IBA:GO_Central.
DR GO; GO:0106070; P:regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0040008; P:regulation of growth; IGI:ZFIN.
DR InterPro; IPR028111; MRAP.
DR PANTHER; PTHR28675; PTHR28675; 1.
DR Pfam; PF15183; MRAP; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Endoplasmic reticulum; Glycoprotein; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..217
FT /note="Melanocortin-2 receptor accessory protein 2A"
FT /id="PRO_0000424028"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 8
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 217 AA; 24354 MW; 7199FCA7A3087916 CRC64;
MPRFQLSNST SVPNHNYEWS YEYYDDEEPV SFEGLKAHRY SIVIGFWVGL AVFVIFMFFV
LTLLTKTGAP HPEAAEPYEK RMRLTSCADG LGRQRETDGR TGLSRPLLEE SRSLFHCYIN
EEEREGGRAA TDAGALTHGR SGIGNSRGQV EEVGLVVQNM VLESRAEREA ALLAHFNIPN
FVNSELNSAL GDEDLLLGDP PIIMEEARPR CTHHIID
