MRPF_ALKPO
ID MRPF_ALKPO Reviewed; 91 AA.
AC Q9RGZ0; D3FXH7;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Na(+)/H(+) antiporter subunit F;
DE AltName: Full=Mrp complex subunit F;
DE AltName: Full=Multiple resistance and pH homeostasis protein F;
GN Name=mrpF; OrderedLocusNames=BpOF4_13185;
OS Alkalihalophilus pseudofirmus (strain ATCC BAA-2126 / JCM 17055 / OF4)
OS (Bacillus pseudofirmus).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalophilus.
OX NCBI_TaxID=398511;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11356194; DOI=10.1016/s0014-5793(01)02417-6;
RA Ito M., Guffanti A.A., Krulwich T.A.;
RT "Mrp-dependent Na(+)/H(+) antiporters of Bacillus exhibit characteristics
RT that are unanticipated for completely secondary active transporters.";
RL FEBS Lett. 496:117-120(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-2126 / JCM 17055 / OF4;
RX PubMed=21951522; DOI=10.1111/j.1462-2920.2011.02591.x;
RA Janto B., Ahmed A., Ito M., Liu J., Hicks D.B., Pagni S., Fackelmayer O.J.,
RA Smith T.A., Earl J., Elbourne L.D., Hassan K., Paulsen I.T., Kolsto A.B.,
RA Tourasse N.J., Ehrlich G.D., Boissy R., Ivey D.M., Li G., Xue Y., Ma Y.,
RA Hu F.Z., Krulwich T.A.;
RT "Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that
RT support the ability to grow in an external pH range from 7.5 to 11.4.";
RL Environ. Microbiol. 13:3289-3309(2011).
RN [3]
RP CHARACTERIZATION, AND PROBABLE FUNCTION IN ELECTROGENIC ANTIPORTER
RP ACTIVITY.
RX PubMed=17293423; DOI=10.1128/jb.00021-07;
RA Swartz T.H., Ito M., Ohira T., Natsui S., Hicks D.B., Krulwich T.A.;
RT "Catalytic properties of Staphylococcus aureus and Bacillus members of the
RT secondary cation/proton antiporter-3 (Mrp) family are revealed by an
RT optimized assay in an Escherichia coli host.";
RL J. Bacteriol. 189:3081-3090(2007).
CC -!- FUNCTION: Mnh complex is a Na(+)Li(+)/H(+) antiporter involved in Na(+)
CC and/or Li(+) excretion and Na(+) resistance. Na(+)/H(+) antiport
CC consumes a transmembrane electrical potential, and is thus inferred to
CC be electrogenic. Does not transport K(+), Ca(2+) or Mg(2+).
CC -!- SUBUNIT: Forms a heterooligomeric complex that consists of seven
CC subunits: MrpA, MrpB, MrpC, MrpD, MrpE, MrpF and MrpG. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Mrp-dependent antiport apparently occurs by a secondary,
CC proton motive force-dependent mechanism, but the similarity of several
CC Mrp proteins to membrane-embedded subunits of energy-coupled NADH
CC dehydrogenase complexes raises the possibility that there is a capacity
CC for electron transport that could provide a primary energy coupling
CC option for Mrp functions.
CC -!- SIMILARITY: Belongs to the CPA3 antiporters (TC 2.A.63) subunit F
CC family. {ECO:0000305}.
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DR EMBL; AF097740; AAF21817.1; -; Genomic_DNA.
DR EMBL; CP001878; ADC50688.1; -; Genomic_DNA.
DR RefSeq; WP_012958051.1; NC_013791.2.
DR AlphaFoldDB; Q9RGZ0; -.
DR SMR; Q9RGZ0; -.
DR STRING; 398511.BpOF4_13185; -.
DR EnsemblBacteria; ADC50688; ADC50688; BpOF4_13185.
DR KEGG; bpf:BpOF4_13185; -.
DR eggNOG; COG2212; Bacteria.
DR HOGENOM; CLU_125825_1_3_9; -.
DR OMA; VIAFARY; -.
DR OrthoDB; 2083248at2; -.
DR Proteomes; UP000001544; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0015075; F:ion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR007208; MrpF/PhaF-like.
DR PANTHER; PTHR34702; PTHR34702; 1.
DR Pfam; PF04066; MrpF_PhaF; 1.
DR PIRSF; PIRSF028784; MrpF; 1.
PE 1: Evidence at protein level;
KW Antiport; Cell membrane; Hydrogen ion transport; Ion transport; Membrane;
KW Reference proteome; Sodium; Sodium transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..91
FT /note="Na(+)/H(+) antiporter subunit F"
FT /id="PRO_0000087743"
FT TRANSMEM 5..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 34..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 63..82
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 91 AA; 10019 MW; F51372924893AC79 CRC64;
MFQSILMIVL VVMSISLFVC FIRTLIGPTM SDRIVALDTF GINLIGFIGV IMMLQETLAY
SEVVLVISIL AFIGSIALSK FIERGVVFDR G
