ID   MRPG_ALKPO              Reviewed;         119 AA.
AC   Q9RGY9; D3FXH6;
DT   13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Na(+)/H(+) antiporter subunit G;
DE   AltName: Full=Mrp complex subunit G;
DE   AltName: Full=Multiple resistance and pH homeostasis protein G;
GN   Name=mrpG; OrderedLocusNames=BpOF4_13180;
OS   Alkalihalophilus pseudofirmus (strain ATCC BAA-2126 / JCM 17055 / OF4)
OS   (Bacillus pseudofirmus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalophilus.
OX   NCBI_TaxID=398511;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11356194; DOI=10.1016/s0014-5793(01)02417-6;
RA   Ito M., Guffanti A.A., Krulwich T.A.;
RT   "Mrp-dependent Na(+)/H(+) antiporters of Bacillus exhibit characteristics
RT   that are unanticipated for completely secondary active transporters.";
RL   FEBS Lett. 496:117-120(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-2126 / JCM 17055 / OF4;
RX   PubMed=21951522; DOI=10.1111/j.1462-2920.2011.02591.x;
RA   Janto B., Ahmed A., Ito M., Liu J., Hicks D.B., Pagni S., Fackelmayer O.J.,
RA   Smith T.A., Earl J., Elbourne L.D., Hassan K., Paulsen I.T., Kolsto A.B.,
RA   Tourasse N.J., Ehrlich G.D., Boissy R., Ivey D.M., Li G., Xue Y., Ma Y.,
RA   Hu F.Z., Krulwich T.A.;
RT   "Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that
RT   support the ability to grow in an external pH range from 7.5 to 11.4.";
RL   Environ. Microbiol. 13:3289-3309(2011).
RN   [3]
RP   CHARACTERIZATION, AND PROBABLE FUNCTION IN ELECTROGENIC ANTIPORTER
RP   ACTIVITY.
RX   PubMed=17293423; DOI=10.1128/jb.00021-07;
RA   Swartz T.H., Ito M., Ohira T., Natsui S., Hicks D.B., Krulwich T.A.;
RT   "Catalytic properties of Staphylococcus aureus and Bacillus members of the
RT   secondary cation/proton antiporter-3 (Mrp) family are revealed by an
RT   optimized assay in an Escherichia coli host.";
RL   J. Bacteriol. 189:3081-3090(2007).
CC   -!- FUNCTION: Mnh complex is a Na(+)Li(+)/H(+) antiporter involved in Na(+)
CC       and/or Li(+) excretion and Na(+) resistance. Na(+)/H(+) antiport
CC       consumes a transmembrane electrical potential, and is thus inferred to
CC       be electrogenic. Does not transport K(+), Ca(2+) or Mg(2+).
CC   -!- SUBUNIT: Forms a heterooligomeric complex that consists of seven
CC       subunits: MrpA, MrpB, MrpC, MrpD, MrpE, MrpF and MrpG. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- MISCELLANEOUS: Mrp-dependent antiport apparently occurs by a secondary,
CC       proton motive force-dependent mechanism, but the similarity of several
CC       Mrp proteins to membrane-embedded subunits of energy-coupled NADH
CC       dehydrogenase complexes raises the possibility that there is a capacity
CC       for electron transport that could provide a primary energy coupling
CC       option for Mrp functions.
CC   -!- SIMILARITY: Belongs to the CPA3 antiporters (TC 2.A.63) subunit G
CC       family. {ECO:0000305}.
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DR   EMBL; AF097740; AAF21818.1; -; Genomic_DNA.
DR   EMBL; CP001878; ADC50687.1; -; Genomic_DNA.
DR   RefSeq; WP_012958050.1; NC_013791.2.
DR   AlphaFoldDB; Q9RGY9; -.
DR   SMR; Q9RGY9; -.
DR   STRING; 398511.BpOF4_13180; -.
DR   EnsemblBacteria; ADC50687; ADC50687; BpOF4_13180.
DR   KEGG; bpf:BpOF4_13180; -.
DR   eggNOG; COG1320; Bacteria.
DR   HOGENOM; CLU_121334_0_3_9; -.
DR   OMA; HMLAKAA; -.
DR   OrthoDB; 2051355at2; -.
DR   Proteomes; UP000001544; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005451; F:monovalent cation:proton antiporter activity; IEA:InterPro.
DR   GO; GO:0098662; P:inorganic cation transmembrane transport; IEA:InterPro.
DR   GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR005133; PhaG_MnhG_YufB.
DR   PANTHER; PTHR34703; PTHR34703; 1.
DR   Pfam; PF03334; PhaG_MnhG_YufB; 1.
DR   TIGRFAMs; TIGR01300; CPA3_mnhG_phaG; 1.
PE   1: Evidence at protein level;
KW   Antiport; Cell membrane; Hydrogen ion transport; Ion transport; Membrane;
KW   Reference proteome; Sodium; Sodium transport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..119
FT                   /note="Na(+)/H(+) antiporter subunit G"
FT                   /id="PRO_0000086863"
FT   TRANSMEM        7..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        44..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        66..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   119 AA;  13108 MW;  611E36006F168628 CRC64;
     MTAVEIIISI FVLIGGFLSL LGSIGIIRFP DVYGRLHAAT KSATLGVISI MLATFLFFFL
     VHGEFVGKLL LTILFVFLTA PVAGMMMGRS AYRVGVPLWE KSTQDDLKKM YEKKMKGSN