MRR_ECOLI
ID MRR_ECOLI Reviewed; 304 AA.
AC P24202; Q2M5W5;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-1992, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Type IV methyl-directed restriction enzyme EcoKMrr {ECO:0000303|PubMed:12654995};
DE Short=EcoKMrr {ECO:0000303|PubMed:12654995};
DE AltName: Full=Mrr restriction system protein;
GN Name=mrr; OrderedLocusNames=b4351, JW4314;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-35.
RC STRAIN=K12;
RX PubMed=1650347; DOI=10.1128/jb.173.16.5207-5219.1991;
RA Waite-Rees P.A., Keating C.J., Moran L.S., Slatko B.E., Hornstra L.J.,
RA Benner J.S.;
RT "Characterization and expression of the Escherichia coli Mrr restriction
RT system.";
RL J. Bacteriol. 173:5207-5219(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP IDENTIFICATION BY 2D-GEL.
RX PubMed=9298644; DOI=10.1002/elps.1150180805;
RA VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.;
RT "Escherichia coli proteome analysis using the gene-protein database.";
RL Electrophoresis 18:1243-1251(1997).
RN [6]
RP NOMENCLATURE.
RX PubMed=12654995; DOI=10.1093/nar/gkg274;
RA Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT endonucleases and their genes.";
RL Nucleic Acids Res. 31:1805-1812(2003).
CC -!- FUNCTION: Involved in the acceptance of foreign DNA which is modified.
CC Restricts both adenine- and cytosine-methylated DNA.
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DR EMBL; X54198; CAA38117.1; -; Genomic_DNA.
DR EMBL; U14003; AAA97248.1; -; Genomic_DNA.
DR EMBL; U00096; AAC77307.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78341.1; -; Genomic_DNA.
DR PIR; A40368; A40368.
DR RefSeq; NP_418771.1; NC_000913.3.
DR RefSeq; WP_000217931.1; NZ_LN832404.1.
DR AlphaFoldDB; P24202; -.
DR BioGRID; 4262770; 58.
DR DIP; DIP-10259N; -.
DR IntAct; P24202; 1.
DR STRING; 511145.b4351; -.
DR REBASE; 13381; EcoW3110MrrP.
DR REBASE; 152622; Rsp6212MrrP.
DR REBASE; 152626; Rsp621MrrP.
DR REBASE; 152632; Ret561MrrP.
DR REBASE; 152643; Rsp1341MrrP.
DR REBASE; 152655; Rsp113MrrP.
DR REBASE; 152716; Rsp741MrrP.
DR REBASE; 152738; Rsp871MrrP.
DR REBASE; 203759; Lbr1174ORF1924P.
DR REBASE; 211852; Rsp894MrrP.
DR REBASE; 211890; Rsp894Mrr2P.
DR REBASE; 2906; EcoKMrr.
DR REBASE; 296186; Bve83MrrP.
DR REBASE; 441389; EcoBL21FMrrP.
DR jPOST; P24202; -.
DR PaxDb; P24202; -.
DR PRIDE; P24202; -.
DR EnsemblBacteria; AAC77307; AAC77307; b4351.
DR EnsemblBacteria; BAE78341; BAE78341; BAE78341.
DR GeneID; 948898; -.
DR KEGG; ecj:JW4314; -.
DR KEGG; eco:b4351; -.
DR PATRIC; fig|1411691.4.peg.2335; -.
DR EchoBASE; EB0607; -.
DR eggNOG; COG1715; Bacteria.
DR HOGENOM; CLU_063822_2_0_6; -.
DR InParanoid; P24202; -.
DR OMA; VIYIQAK; -.
DR PhylomeDB; P24202; -.
DR BioCyc; EcoCyc:EG10612-MON; -.
DR PRO; PR:P24202; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0043590; C:bacterial nucleoid; IDA:EcoCyc.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0015666; F:restriction endodeoxyribonuclease activity; IMP:EcoCyc.
DR GO; GO:0032067; F:type IV site-specific deoxyribonuclease activity; IDA:EcoCyc.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR GO; GO:0051599; P:response to hydrostatic pressure; IMP:EcoCyc.
DR Gene3D; 3.40.1350.10; -; 1.
DR InterPro; IPR025745; Mrr-like_N_dom.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR007560; Restrct_endonuc_IV_Mrr.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR Pfam; PF04471; Mrr_cat; 1.
DR Pfam; PF14338; Mrr_N; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Reference proteome; Restriction system.
FT CHAIN 1..304
FT /note="Type IV methyl-directed restriction enzyme EcoKMrr"
FT /id="PRO_0000077382"
FT REGION 120..142
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 304 AA; 33520 MW; 6DE16E229E9EC9AE CRC64;
MTVPTYDKFI EPVLRYLATK PEGAAARDVH EAAADALGLD DSQRAKVITS GQLVYKNRAG
WAHDRLKRAG LSQSLSRGKW CLTPAGFDWV ASHPQPMTEQ ETNHLAFAFV NVKLKSRPDA
VDLDPKADSP DHEELAKSSP DDRLDQALKE LRDAVADEVL ENLLQVSPSR FEVIVLDVLH
RLGYGGHRDD LQRVGGTGDG GIDGVISLDK LGLEKVYVQA KRWQNTVGRP ELQAFYGALA
GQKAKRGVFI TTSGFTSQAR DFAQSVEGMV LVDGERLVHL MIENEVGVSS RLLKVPKLDM
DYFE
