MRS21_ARATH
ID MRS21_ARATH Reviewed; 442 AA.
AC Q9S9N4;
DT 18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Magnesium transporter MRS2-1;
DE AltName: Full=Magnesium Transporter 2;
DE Short=AtMGT2;
GN Name=MRS2-1; Synonyms=MGT2; OrderedLocusNames=At1g16010;
GN ORFNames=T24D18.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=11115130; DOI=10.1046/j.1365-313x.2000.00895.x;
RA Schock I., Gregan J., Steinhauser S., Schweyen R., Brennicke A., Knoop V.;
RT "A member of a novel Arabidopsis thaliana gene family of candidate Mg2+ ion
RT transporters complements a yeast mitochondrial group II intron-splicing
RT mutant.";
RL Plant J. 24:489-501(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=11752386; DOI=10.2307/3871533;
RA Li L., Tutone A.F., Drummond R.S., Gardner R.C., Luan S.;
RT "A novel family of magnesium transport genes in Arabidopsis.";
RL Plant Cell 13:2761-2775(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia; TISSUE=Rosette leaf;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [7]
RP GENE FAMILY, NOMENCLATURE, AND TISSUE SPECIFICITY.
RX PubMed=19966073; DOI=10.1105/tpc.109.070557;
RA Gebert M., Meschenmoser K., Svidova S., Weghuber J., Schweyen R.,
RA Eifler K., Lenz H., Weyand K., Knoop V.;
RT "A root-expressed magnesium transporter of the MRS2/MGT gene family in
RT Arabidopsis thaliana allows for growth in low-Mg2+ environments.";
RL Plant Cell 21:4018-4030(2009).
CC -!- FUNCTION: Magnesium transporter that may mediate the influx of
CC magnesium.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in the whole plant except stems.
CC {ECO:0000269|PubMed:11115130, ECO:0000269|PubMed:11752386,
CC ECO:0000269|PubMed:19966073}.
CC -!- MISCELLANEOUS: Has the ability to complement a mutant in yeast lacking
CC magnesium transport capability.
CC -!- SIMILARITY: Belongs to the CorA metal ion transporter (MIT) (TC
CC 1.A.35.5) family. {ECO:0000305}.
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DR EMBL; AJ297816; CAC13981.1; -; mRNA.
DR EMBL; AY150286; AAN73211.1; -; mRNA.
DR EMBL; AC010924; AAF18497.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29395.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29396.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29397.1; -; Genomic_DNA.
DR EMBL; AY054657; AAK96848.1; -; mRNA.
DR EMBL; AY081530; AAM10092.1; -; mRNA.
DR EMBL; AK318948; BAH57063.1; -; mRNA.
DR EMBL; AK319031; BAH57146.1; -; mRNA.
DR PIR; G86294; G86294.
DR RefSeq; NP_001077545.1; NM_001084076.1.
DR RefSeq; NP_001185007.1; NM_001198078.1.
DR RefSeq; NP_563988.1; NM_101469.1.
DR AlphaFoldDB; Q9S9N4; -.
DR SMR; Q9S9N4; -.
DR BioGRID; 23411; 7.
DR IntAct; Q9S9N4; 6.
DR STRING; 3702.AT1G16010.2; -.
DR iPTMnet; Q9S9N4; -.
DR PaxDb; Q9S9N4; -.
DR PRIDE; Q9S9N4; -.
DR ProteomicsDB; 238908; -.
DR EnsemblPlants; AT1G16010.1; AT1G16010.1; AT1G16010.
DR EnsemblPlants; AT1G16010.2; AT1G16010.2; AT1G16010.
DR EnsemblPlants; AT1G16010.3; AT1G16010.3; AT1G16010.
DR GeneID; 838171; -.
DR Gramene; AT1G16010.1; AT1G16010.1; AT1G16010.
DR Gramene; AT1G16010.2; AT1G16010.2; AT1G16010.
DR Gramene; AT1G16010.3; AT1G16010.3; AT1G16010.
DR KEGG; ath:AT1G16010; -.
DR Araport; AT1G16010; -.
DR TAIR; locus:2200542; AT1G16010.
DR eggNOG; KOG2662; Eukaryota.
DR HOGENOM; CLU_034694_1_1_1; -.
DR InParanoid; Q9S9N4; -.
DR OMA; RNFDNVF; -.
DR OrthoDB; 1363588at2759; -.
DR PhylomeDB; Q9S9N4; -.
DR PRO; PR:Q9S9N4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9S9N4; baseline and differential.
DR Genevisible; Q9S9N4; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015693; P:magnesium ion transport; IBA:GO_Central.
DR CDD; cd12823; Mrs2_Mfm1p-like; 1.
DR InterPro; IPR045863; CorA_TM1_TM2.
DR InterPro; IPR002523; MgTranspt_CorA/ZnTranspt_ZntB.
DR InterPro; IPR039204; MRS2-like.
DR PANTHER; PTHR13890; PTHR13890; 1.
DR Pfam; PF01544; CorA; 1.
DR SUPFAM; SSF144083; SSF144083; 1.
PE 2: Evidence at transcript level;
KW Ion transport; Magnesium; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..442
FT /note="Magnesium transporter MRS2-1"
FT /id="PRO_0000394165"
FT TRANSMEM 378..398
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 414..434
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 398..400
FT /note="Required for magnesium transport activity"
SQ SEQUENCE 442 AA; 50420 MW; 92E43A437DFAC006 CRC64;
MSELKERLLP PRPASAMNLR DASVTRPSAS GRPPLLGVDV LGLKKRGQGL RSWIRVDTSG
NTQVMEVDKF TMMRRCDLPA RDLRLLDPLF VYPSTILGRE KAIVVNLEQI RCIITADEVL
LLNSLDNYVL RYVVELQQRL KTSSVGEMWQ QENSQLSRRR SRSFDNAFEN SSPDYLPFEF
RALEIALEAA CTFLDSQASE LEIEAYPLLD ELTSKISTLN LERVRRLKSR LVALTRRVQK
VRDEIEQLMD DDGDMAEMYL TEKKRRMEGS MYGDQSLLGY RSNDGLSVSA PVSPVSSPPD
SRRLDKSLSI ARSRHDSARS SEGAENIEEL EMLLEAYFVV IDSTLNKLTS LKEYIDDTED
FINIQLDNVR NQLIQFELLL TTATFVVAIF GVVAGIFGMN FEIDFFNQPG AFRWVLIITG
VCGFVIFSAF VWFFKYRRLM PL
