MRS22_ARATH
ID MRS22_ARATH Reviewed; 394 AA.
AC Q9FLG2; Q9FT79;
DT 18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Magnesium transporter MRS2-2;
DE AltName: Full=Magnesium Transporter 9;
DE Short=AtMGT9;
GN Name=MRS2-2; Synonyms=MGT9; OrderedLocusNames=At5g64560; ORFNames=MUB3.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND TISSUE SPECIFICITY.
RX PubMed=11115130; DOI=10.1046/j.1365-313x.2000.00895.x;
RA Schock I., Gregan J., Steinhauser S., Schweyen R., Brennicke A., Knoop V.;
RT "A member of a novel Arabidopsis thaliana gene family of candidate Mg2+ ion
RT transporters complements a yeast mitochondrial group II intron-splicing
RT mutant.";
RL Plant J. 24:489-501(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, AND TISSUE
RP SPECIFICITY.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=11752386; DOI=10.2307/3871533;
RA Li L., Tutone A.F., Drummond R.S., Gardner R.C., Luan S.;
RT "A novel family of magnesium transport genes in Arabidopsis.";
RL Plant Cell 13:2761-2775(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=19436262; DOI=10.1038/cr.2009.58;
RA Chen J., Li L.G., Liu Z.H., Yuan Y.J., Guo L.L., Mao D.D., Tian L.F.,
RA Chen L.B., Luan S., Li D.P.;
RT "Magnesium transporter AtMGT9 is essential for pollen development in
RT Arabidopsis.";
RL Cell Res. 19:887-898(2009).
RN [8]
RP GENE FAMILY, NOMENCLATURE, AND TISSUE SPECIFICITY.
RX PubMed=19966073; DOI=10.1105/tpc.109.070557;
RA Gebert M., Meschenmoser K., Svidova S., Weghuber J., Schweyen R.,
RA Eifler K., Lenz H., Weyand K., Knoop V.;
RT "A root-expressed magnesium transporter of the MRS2/MGT gene family in
RT Arabidopsis thaliana allows for growth in low-Mg2+ environments.";
RL Plant Cell 21:4018-4030(2009).
CC -!- FUNCTION: Low-affinity magnesium transporter that mediates the influx
CC of magnesium. Plays a crucial role in male gametophyte development and
CC male fertility. {ECO:0000269|PubMed:19436262}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9FLG2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9FLG2-2; Sequence=VSP_039190, VSP_039191;
CC -!- TISSUE SPECIFICITY: Expressed in the whole plant but preferentially in
CC the mature anthers. {ECO:0000269|PubMed:11115130,
CC ECO:0000269|PubMed:11752386, ECO:0000269|PubMed:19436262,
CC ECO:0000269|PubMed:19966073}.
CC -!- MISCELLANEOUS: Has the ability to complement mutants in Salmonella
CC enterica and yeast lacking magnesium transport capability.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to an intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the CorA metal ion transporter (MIT) (TC
CC 1.A.35.5) family. {ECO:0000305}.
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DR EMBL; AJ297817; CAC13982.1; -; mRNA.
DR EMBL; AY150287; AAN73212.1; -; mRNA.
DR EMBL; AB010076; BAB11423.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97920.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97921.1; -; Genomic_DNA.
DR EMBL; AY065449; AAL38890.1; -; mRNA.
DR EMBL; AY096449; AAM20089.1; -; mRNA.
DR EMBL; AY084382; AAM60960.1; -; mRNA.
DR RefSeq; NP_201261.2; NM_125852.2. [Q9FLG2-2]
DR RefSeq; NP_851269.1; NM_180938.3. [Q9FLG2-1]
DR AlphaFoldDB; Q9FLG2; -.
DR SMR; Q9FLG2; -.
DR BioGRID; 21819; 28.
DR IntAct; Q9FLG2; 28.
DR STRING; 3702.AT5G64560.1; -.
DR PaxDb; Q9FLG2; -.
DR PRIDE; Q9FLG2; -.
DR ProteomicsDB; 238909; -. [Q9FLG2-1]
DR EnsemblPlants; AT5G64560.1; AT5G64560.1; AT5G64560. [Q9FLG2-1]
DR EnsemblPlants; AT5G64560.2; AT5G64560.2; AT5G64560. [Q9FLG2-2]
DR GeneID; 836577; -.
DR Gramene; AT5G64560.1; AT5G64560.1; AT5G64560. [Q9FLG2-1]
DR Gramene; AT5G64560.2; AT5G64560.2; AT5G64560. [Q9FLG2-2]
DR KEGG; ath:AT5G64560; -.
DR Araport; AT5G64560; -.
DR TAIR; locus:2174799; AT5G64560.
DR eggNOG; KOG2662; Eukaryota.
DR InParanoid; Q9FLG2; -.
DR OMA; TQHDVEG; -.
DR PhylomeDB; Q9FLG2; -.
DR PRO; PR:Q9FLG2; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FLG2; baseline and differential.
DR Genevisible; Q9FLG2; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015693; P:magnesium ion transport; IBA:GO_Central.
DR GO; GO:0009555; P:pollen development; IMP:TAIR.
DR CDD; cd12823; Mrs2_Mfm1p-like; 1.
DR InterPro; IPR045863; CorA_TM1_TM2.
DR InterPro; IPR002523; MgTranspt_CorA/ZnTranspt_ZntB.
DR InterPro; IPR039204; MRS2-like.
DR PANTHER; PTHR13890; PTHR13890; 1.
DR Pfam; PF01544; CorA; 1.
DR SUPFAM; SSF144083; SSF144083; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Ion transport; Magnesium; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..394
FT /note="Magnesium transporter MRS2-2"
FT /id="PRO_0000394166"
FT TRANSMEM 329..349
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 366..386
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 115..145
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 349..351
FT /note="Required for magnesium transport activity"
FT VAR_SEQ 368..378
FT /note="VGLTGTLCVVV -> RNQNSIRMRMK (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11115130"
FT /id="VSP_039190"
FT VAR_SEQ 379..394
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11115130"
FT /id="VSP_039191"
SQ SEQUENCE 394 AA; 43720 MW; 328E64E5A8D6FB77 CRC64;
MAQNGYLVPA DPSAVVTVKK KTPQASWALI DATGQSEPLD VDKYEIMHRV QIHARDLRIL
DPNLSYPSTI LGRERAIVLN LEHIKAIITS EEVLLRDPSD ENVIPVVEEL RRRLPVGNAS
HNGGQGDGKE IAGAQNDGDT GDEDESPFEF RALEVALEAI CSFLAARTAE LETAAYPALD
ELTSKISSRN LDRVRKLKSA MTRLTARVQK VRDELEQLLD DDDDMADLYL SRKLSSASSP
ISSIGEPNWY TTSPTIGSKI SRASRASLAT VHGDENDVEE LEMLLEAYFM QIDSTLNRLT
TLREYIDDTE DYINIQLDNH RNQLIQLELV LSSGTVCLSM YSLVAGIFGM NIPYTWNDGH
GYMFKYVVGL TGTLCVVVFV IIMSYARYKG LVGS
