MRS25_ARATH
ID MRS25_ARATH Reviewed; 421 AA.
AC Q9ZPR4; Q8H1H0;
DT 18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 25-MAY-2022, entry version 117.
DE RecName: Full=Magnesium transporter MRS2-5;
DE AltName: Full=Magnesium Transporter 3;
DE Short=AtMGT3;
GN Name=MRS2-5; Synonyms=MGT3; OrderedLocusNames=At2g03620; ORFNames=F19B11.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=11752386; DOI=10.2307/3871533;
RA Li L., Tutone A.F., Drummond R.S., Gardner R.C., Luan S.;
RT "A novel family of magnesium transport genes in Arabidopsis.";
RL Plant Cell 13:2761-2775(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=12481096; DOI=10.1104/pp.010207;
RA Xiao Y.-L., Malik M., Whitelaw C.A., Town C.D.;
RT "Cloning and sequencing of cDNAs for hypothetical genes from chromosome 2
RT of Arabidopsis.";
RL Plant Physiol. 130:2118-2128(2002).
RN [6]
RP GENE FAMILY, NOMENCLATURE, AND TISSUE SPECIFICITY.
RX PubMed=19966073; DOI=10.1105/tpc.109.070557;
RA Gebert M., Meschenmoser K., Svidova S., Weghuber J., Schweyen R.,
RA Eifler K., Lenz H., Weyand K., Knoop V.;
RT "A root-expressed magnesium transporter of the MRS2/MGT gene family in
RT Arabidopsis thaliana allows for growth in low-Mg2+ environments.";
RL Plant Cell 21:4018-4030(2009).
CC -!- FUNCTION: Magnesium transporter that may mediate the influx of
CC magnesium. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in the whole plant.
CC {ECO:0000269|PubMed:11752386, ECO:0000269|PubMed:19966073}.
CC -!- MISCELLANEOUS: Has the ability to complement a mutant in yeast lacking
CC magnesium transport capability.
CC -!- SIMILARITY: Belongs to the CorA metal ion transporter (MIT) (TC
CC 1.A.35.5) family. {ECO:0000305}.
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DR EMBL; AY150290; AAN73215.1; -; mRNA.
DR EMBL; AC006836; AAD20070.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05725.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05726.1; -; Genomic_DNA.
DR EMBL; AY070380; AAL49876.1; -; mRNA.
DR EMBL; AY096567; AAM20217.1; -; mRNA.
DR EMBL; AF499434; AAM19344.1; -; mRNA.
DR PIR; F84450; F84450.
DR RefSeq; NP_001118259.1; NM_001124787.1.
DR RefSeq; NP_178460.1; NM_126412.6.
DR AlphaFoldDB; Q9ZPR4; -.
DR SMR; Q9ZPR4; -.
DR BioGRID; 292; 38.
DR IntAct; Q9ZPR4; 37.
DR STRING; 3702.AT2G03620.2; -.
DR TCDB; 1.A.35.5.6; the cora metal ion transporter (mit) family.
DR iPTMnet; Q9ZPR4; -.
DR PaxDb; Q9ZPR4; -.
DR PRIDE; Q9ZPR4; -.
DR ProteomicsDB; 250868; -.
DR EnsemblPlants; AT2G03620.1; AT2G03620.1; AT2G03620.
DR EnsemblPlants; AT2G03620.2; AT2G03620.2; AT2G03620.
DR GeneID; 814891; -.
DR Gramene; AT2G03620.1; AT2G03620.1; AT2G03620.
DR Gramene; AT2G03620.2; AT2G03620.2; AT2G03620.
DR KEGG; ath:AT2G03620; -.
DR Araport; AT2G03620; -.
DR TAIR; locus:2044254; AT2G03620.
DR eggNOG; KOG2662; Eukaryota.
DR HOGENOM; CLU_034694_1_0_1; -.
DR InParanoid; Q9ZPR4; -.
DR OMA; FDYPSGF; -.
DR OrthoDB; 1363588at2759; -.
DR PhylomeDB; Q9ZPR4; -.
DR PRO; PR:Q9ZPR4; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZPR4; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015693; P:magnesium ion transport; IBA:GO_Central.
DR CDD; cd12823; Mrs2_Mfm1p-like; 1.
DR InterPro; IPR039204; MRS2-like.
DR PANTHER; PTHR13890; PTHR13890; 1.
PE 2: Evidence at transcript level;
KW Ion transport; Magnesium; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..421
FT /note="Magnesium transporter MRS2-5"
FT /id="PRO_0000394169"
FT TRANSMEM 357..377
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 393..413
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOTIF 377..379
FT /note="Required for magnesium transport activity"
FT CONFLICT 185
FT /note="E -> D (in Ref. 1; AAN73215)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 421 AA; 47035 MW; 5E540739E8906E17 CRC64;
MGEQLDPFSA SNLPDFISSQ KIGRPVNFEG QTNRGHPFSG LKKRGQSSRS WVKIDQDGNS
AVLELDKATI MKRCSLPSRD LRLLDPLFIY PSSILGRERA IVVSLEKIRC IITAEEVILM
NARDASVVQY QSELCKRLQS NHNLNVKDDL PFEFKALELV LELSCLSLDA QVNELEMEVY
PVLDELATNI STLNLEHVRR LKGRLLTLTQ KVQKVCDEIE HLMDDDDDMA EMYLTEKKER
AEAHASEELE DNIGEDFESS GIVSKSAPVS PVGSTSGNFG KLQRAFSSIV GSHKSLLSSS
SIGENIDQLE MLLEAYFVVV DNTLSKLSSL KEYIDDTEDL INIKLGNVQN QLIQFQLLLT
AATFVAAIFA AVTAVFGMNL QDSVFQNPTT FQYVLLITGI GCGFLYFGFV LYFKHKKVFP
L
