MRS26_ARATH
ID MRS26_ARATH Reviewed; 408 AA.
AC Q1PE39; A0MFA6; Q8H1G9; Q9M0H3;
DT 18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Magnesium transporter MRS2-6, mitochondrial;
DE AltName: Full=Magnesium Transporter 5;
DE Short=AtMGT5;
DE Flags: Precursor;
GN Name=MRS2-6; Synonyms=MGT5; OrderedLocusNames=At4g28580; ORFNames=T5F17.30;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=11752386; DOI=10.2307/3871533;
RA Li L., Tutone A.F., Drummond R.S., Gardner R.C., Luan S.;
RT "A novel family of magnesium transport genes in Arabidopsis.";
RL Plant Cell 13:2761-2775(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=19825572; DOI=10.1093/mp/ssn031;
RA Li L.G., Sokolov L.N., Yang Y.H., Li D.P., Ting J., Pandy G.K., Luan S.;
RT "A mitochondrial magnesium transporter functions in Arabidopsis pollen
RT development.";
RL Mol. Plant 1:675-685(2008).
RN [6]
RP GENE FAMILY, NOMENCLATURE, AND TISSUE SPECIFICITY.
RX PubMed=19966073; DOI=10.1105/tpc.109.070557;
RA Gebert M., Meschenmoser K., Svidova S., Weghuber J., Schweyen R.,
RA Eifler K., Lenz H., Weyand K., Knoop V.;
RT "A root-expressed magnesium transporter of the MRS2/MGT gene family in
RT Arabidopsis thaliana allows for growth in low-Mg2+ environments.";
RL Plant Cell 21:4018-4030(2009).
CC -!- FUNCTION: Magnesium transporter that mediates the influx/efflux of
CC magnesium in a concentration-dependent manner. Plays a crucial role in
CC male gametophyte development and male fertility.
CC {ECO:0000269|PubMed:19825572}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane
CC {ECO:0000269|PubMed:19825572}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:19825572}.
CC -!- TISSUE SPECIFICITY: Expressed predominantly in flowers notably in
CC anthers at early stages of flower development.
CC {ECO:0000269|PubMed:11752386, ECO:0000269|PubMed:19825572,
CC ECO:0000269|PubMed:19966073}.
CC -!- MISCELLANEOUS: Has the ability to complement mutants in Salmonella
CC typhimurium and yeast lacking magnesium transport capability.
CC -!- SIMILARITY: Belongs to the CorA metal ion transporter (MIT) (TC
CC 1.A.35.5) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK28655.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
CC Sequence=CAB81446.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY150291; AAN73216.1; -; mRNA.
DR EMBL; AL161573; CAB81446.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE85509.1; -; Genomic_DNA.
DR EMBL; DQ446879; ABE66098.1; -; mRNA.
DR EMBL; DQ653232; ABK28655.1; ALT_SEQ; mRNA.
DR PIR; T10652; T10652.
DR RefSeq; NP_194587.2; NM_119000.3.
DR AlphaFoldDB; Q1PE39; -.
DR SMR; Q1PE39; -.
DR BioGRID; 14263; 4.
DR IntAct; Q1PE39; 4.
DR STRING; 3702.AT4G28580.1; -.
DR PaxDb; Q1PE39; -.
DR PRIDE; Q1PE39; -.
DR ProteomicsDB; 238912; -.
DR EnsemblPlants; AT4G28580.1; AT4G28580.1; AT4G28580.
DR GeneID; 828976; -.
DR Gramene; AT4G28580.1; AT4G28580.1; AT4G28580.
DR KEGG; ath:AT4G28580; -.
DR Araport; AT4G28580; -.
DR TAIR; locus:2139905; AT4G28580.
DR eggNOG; KOG2662; Eukaryota.
DR HOGENOM; CLU_034694_1_0_1; -.
DR InParanoid; Q1PE39; -.
DR OMA; VDTEDYI; -.
DR OrthoDB; 1363588at2759; -.
DR PhylomeDB; Q1PE39; -.
DR PRO; PR:Q1PE39; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q1PE39; baseline and differential.
DR Genevisible; Q1PE39; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015693; P:magnesium ion transport; IBA:GO_Central.
DR GO; GO:0009555; P:pollen development; IMP:TAIR.
DR CDD; cd12823; Mrs2_Mfm1p-like; 1.
DR InterPro; IPR039204; MRS2-like.
DR PANTHER; PTHR13890; PTHR13890; 1.
PE 2: Evidence at transcript level;
KW Ion transport; Magnesium; Membrane; Mitochondrion; Reference proteome;
KW Transit peptide; Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..96
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 97..408
FT /note="Magnesium transporter MRS2-6, mitochondrial"
FT /id="PRO_0000394170"
FT TRANSMEM 345..365
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 378..398
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 365..367
FT /note="Required for magnesium transport activity"
FT CONFLICT 29
FT /note="S -> L (in Ref. 1; AAN73216)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 408 AA; 46021 MW; 557B43A83152DA4C CRC64;
MGSLRRSTSN RSRKKGTAVK MNRMPSSLSP PTPPCSAIVG GTGKSKKRRG GVCLWTRFDR
TGFMEVAGCD KSTIIERSSV SAKDLRTAFS HSSKILAREK AIVLNLEVIK AVITSEQVML
LDSLRPEVLT LTDRLKHHFP RKDGPENILQ ASSHGHQEGG EEGLKSKLPF EFRVLEIAFE
VFCSFVDSNV VDLETQAWSI LDELTKKVSN ENLKDLRSLK TSLTHLLARV QKVRDEIEHF
LDDKEDMEDL YLTRKWIQNQ QTEAASNSIV SQPNLQRHTS NRISTSMVTE EDDIDDMEML
LEAYFMQLEG MRNKILLMKE HIDSTEAYVK ILQNSRRNGL IHLMMLVNIG NYAITAGTVV
VNLFGMNIPI GLYSTPDIFG YVVWAVVALC IVLFIVTVGY AKWKKLLD
