MRS27_ARATH
ID MRS27_ARATH Reviewed; 386 AA.
AC Q304A0; Q0WRL6; Q206Z2; Q303Z9; Q3E9J2; Q8H1G8; Q9FXX5; Q9LXD2;
DT 18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Magnesium transporter MRS2-7;
DE AltName: Full=Magnesium Transporter 7;
DE Short=AtMGT7;
GN Name=MRS2-7; Synonyms=MGT7; OrderedLocusNames=At5g09690;
GN ORFNames=F17I14.120;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, AND TISSUE
RP SPECIFICITY.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=11752386; DOI=10.2307/3871533;
RA Li L., Tutone A.F., Drummond R.S., Gardner R.C., Luan S.;
RT "A novel family of magnesium transport genes in Arabidopsis.";
RL Plant Cell 13:2761-2775(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 4), FUNCTION, AND TISSUE
RP SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=19093971; DOI=10.1111/j.1744-7909.2008.00770.x;
RA Mao D.D., Tian L.F., Li L.G., Chen J., Deng P.Y., Li D.P., Luan S.;
RT "AtMGT7: an Arabidopsis gene encoding a low-affinity magnesium
RT transporter.";
RL J. Integr. Plant Biol. 50:1530-1538(2008).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT clones.";
RL DNA Res. 6:183-195(1999).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=19966073; DOI=10.1105/tpc.109.070557;
RA Gebert M., Meschenmoser K., Svidova S., Weghuber J., Schweyen R.,
RA Eifler K., Lenz H., Weyand K., Knoop V.;
RT "A root-expressed magnesium transporter of the MRS2/MGT gene family in
RT Arabidopsis thaliana allows for growth in low-Mg2+ environments.";
RL Plant Cell 21:4018-4030(2009).
CC -!- FUNCTION: Low-affinity magnesium transporter that mediates the influx
CC of magnesium. {ECO:0000269|PubMed:19093971}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:19966073}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:19966073}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1; Synonyms=MGT7a;
CC IsoId=Q304A0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q304A0-2; Sequence=VSP_039192;
CC Name=3;
CC IsoId=Q304A0-3; Sequence=VSP_039194, VSP_039195;
CC Name=4; Synonyms=MGT7b;
CC IsoId=Q304A0-4; Sequence=VSP_039193;
CC -!- TISSUE SPECIFICITY: Isoform 1 is expressed in the whole plant. Isoform
CC 4 is expressed only in roots and flowers. {ECO:0000269|PubMed:11752386,
CC ECO:0000269|PubMed:19093971, ECO:0000269|PubMed:19966073}.
CC -!- DISRUPTION PHENOTYPE: Displays plantlets with retarded development
CC under low Mg(2+) concentrations growth conditions.
CC {ECO:0000269|PubMed:19966073}.
CC -!- MISCELLANEOUS: Has the ability to complement mutants in Salmonella
CC enterica and yeast lacking magnesium transport capability.
CC -!- SIMILARITY: Belongs to the CorA metal ion transporter (MIT) (TC
CC 1.A.35.5) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB09524.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BX831230; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
CC Sequence=CAB89361.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY150292; AAN73217.1; -; mRNA.
DR EMBL; DQ408372; ABD64135.2; -; mRNA.
DR EMBL; DQ408373; ABD64136.3; -; mRNA.
DR EMBL; AB020752; BAB09524.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL353994; CAB89361.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED91431.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91432.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91433.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91434.1; -; Genomic_DNA.
DR EMBL; BX831230; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK228287; BAF00233.1; -; mRNA.
DR PIR; T49929; T49929.
DR RefSeq; NP_001078557.1; NM_001085088.1. [Q304A0-4]
DR RefSeq; NP_196531.2; NM_121006.4. [Q304A0-1]
DR RefSeq; NP_850802.2; NM_180471.3. [Q304A0-2]
DR RefSeq; NP_974757.2; NM_203028.3. [Q304A0-3]
DR AlphaFoldDB; Q304A0; -.
DR SMR; Q304A0; -.
DR BioGRID; 16106; 2.
DR IntAct; Q304A0; 2.
DR STRING; 3702.AT5G09690.2; -.
DR iPTMnet; Q304A0; -.
DR PaxDb; Q304A0; -.
DR PRIDE; Q304A0; -.
DR ProteomicsDB; 250869; -. [Q304A0-1]
DR EnsemblPlants; AT5G09690.1; AT5G09690.1; AT5G09690. [Q304A0-1]
DR EnsemblPlants; AT5G09690.2; AT5G09690.2; AT5G09690. [Q304A0-2]
DR EnsemblPlants; AT5G09690.3; AT5G09690.3; AT5G09690. [Q304A0-3]
DR EnsemblPlants; AT5G09690.4; AT5G09690.4; AT5G09690. [Q304A0-4]
DR GeneID; 830828; -.
DR Gramene; AT5G09690.1; AT5G09690.1; AT5G09690. [Q304A0-1]
DR Gramene; AT5G09690.2; AT5G09690.2; AT5G09690. [Q304A0-2]
DR Gramene; AT5G09690.3; AT5G09690.3; AT5G09690. [Q304A0-3]
DR Gramene; AT5G09690.4; AT5G09690.4; AT5G09690. [Q304A0-4]
DR KEGG; ath:AT5G09690; -.
DR Araport; AT5G09690; -.
DR TAIR; locus:2144826; AT5G09690.
DR eggNOG; KOG2662; Eukaryota.
DR InParanoid; Q304A0; -.
DR OrthoDB; 1363588at2759; -.
DR PhylomeDB; Q304A0; -.
DR PRO; PR:Q304A0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q304A0; baseline and differential.
DR Genevisible; Q304A0; AT.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015693; P:magnesium ion transport; IBA:GO_Central.
DR CDD; cd12823; Mrs2_Mfm1p-like; 1.
DR InterPro; IPR045863; CorA_TM1_TM2.
DR InterPro; IPR002523; MgTranspt_CorA/ZnTranspt_ZntB.
DR InterPro; IPR039204; MRS2-like.
DR PANTHER; PTHR13890; PTHR13890; 1.
DR Pfam; PF01544; CorA; 1.
DR SUPFAM; SSF144083; SSF144083; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Endoplasmic reticulum; Ion transport; Magnesium;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..386
FT /note="Magnesium transporter MRS2-7"
FT /id="PRO_0000394171"
FT TRANSMEM 321..341
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 355..375
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOTIF 341..343
FT /note="Required for magnesium transport activity"
FT VAR_SEQ 1
FT /note="M -> MALGLSSEESEM (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14993207"
FT /id="VSP_039192"
FT VAR_SEQ 278..292
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:19093971"
FT /id="VSP_039193"
FT VAR_SEQ 319..327
FT /note="LELMLSAGT -> EPSVYQCTL (in isoform 3)"
FT /evidence="ECO:0000303|Ref.7"
FT /id="VSP_039194"
FT VAR_SEQ 328..386
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|Ref.7"
FT /id="VSP_039195"
FT CONFLICT 122..125
FT /note="AHGV -> EHGA (in Ref. 1; AAN73217)"
FT /evidence="ECO:0000305"
FT CONFLICT 131
FT /note="L -> V (in Ref. 1; AAN73217)"
FT /evidence="ECO:0000305"
FT CONFLICT 165
FT /note="F -> S (in Ref. 1; AAN73217)"
FT /evidence="ECO:0000305"
FT CONFLICT 257..260
FT /note="VSLV -> MSLA (in Ref. 1; AAN73217)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 386 AA; 43431 MW; CF35EDEF549E58FD CRC64;
MSPDGELVPV DSSAVVTAKR KTSQLSRSWI SIDATGQKTV LDVDKHVIMH RVQIHARDLR
ILDPNLFYPS AILGRERAIV LNLEHIKAII TAEEVLIRDS SDENVIPVLE EFQRRLPVGN
EAHGVHGDGD LGEEDESPFE FRALEVALEA ICSFLAARTT ELEKFAYPAL DELTLKISSR
NLERVRKLKS AMTRLTARVQ KVRDELEQLL DDDGDMADLY LTRKLVGASS SVSVSDEPIW
YPTSPTIGSM ISRASRVSLV TVRGDDETDV EELEMLLEAY FMQIDSTLNK LTELREYIDD
TEDYINIQLD NHRNQLIQLE LMLSAGTVCV SVYSMIAGIF GMNIPNTWNH DHGYIFKWVV
SLTGTFCIVL FVIILSYARF RGLIGS
