MRS2A_ARATH
ID MRS2A_ARATH Reviewed; 443 AA.
AC Q9SAH0; Q56ZB1; Q8LE03;
DT 18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Magnesium transporter MRS2-10;
DE AltName: Full=Magnesium Transporter 1;
DE Short=AtMGT1;
GN Name=MRS2-10; Synonyms=MGT1; OrderedLocusNames=At1g80900;
GN ORFNames=F23A5.26;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, FUNCTION, SUBCELLULAR LOCATION,
RP AND TISSUE SPECIFICITY.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=11752386; DOI=10.2307/3871533;
RA Li L., Tutone A.F., Drummond R.S., Gardner R.C., Luan S.;
RT "A novel family of magnesium transport genes in Arabidopsis.";
RL Plant Cell 13:2761-2775(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 106-443.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION.
RX PubMed=17101715; DOI=10.1093/jxb/erl201;
RA Deng W., Luo K., Li D., Zheng X., Wei X., Smith W., Thammina C., Lu L.,
RA Li Y., Pei Y.;
RT "Overexpression of an Arabidopsis magnesium transport gene, AtMGT1, in
RT Nicotiana benthamiana confers Al tolerance.";
RL J. Exp. Bot. 57:4235-4243(2006).
RN [7]
RP GENE FAMILY, NOMENCLATURE, AND TISSUE SPECIFICITY.
RX PubMed=19966073; DOI=10.1105/tpc.109.070557;
RA Gebert M., Meschenmoser K., Svidova S., Weghuber J., Schweyen R.,
RA Eifler K., Lenz H., Weyand K., Knoop V.;
RT "A root-expressed magnesium transporter of the MRS2/MGT gene family in
RT Arabidopsis thaliana allows for growth in low-Mg2+ environments.";
RL Plant Cell 21:4018-4030(2009).
CC -!- FUNCTION: High-affinity magnesium transporter that mediates the influx
CC of magnesium. Involved in tolerance to Aluminum.
CC {ECO:0000269|PubMed:11752386, ECO:0000269|PubMed:17101715}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:11752386};
CC Multi-pass membrane protein {ECO:0000269|PubMed:11752386}.
CC -!- TISSUE SPECIFICITY: Expressed in the whole plant.
CC {ECO:0000269|PubMed:11752386, ECO:0000269|PubMed:19966073}.
CC -!- MISCELLANEOUS: Has the ability to complement mutants in Salmonella
CC enterica and yeast lacking magnesium transport capability.
CC -!- SIMILARITY: Belongs to the CorA metal ion transporter (MIT) (TC
CC 1.A.35.5) family. {ECO:0000305}.
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DR EMBL; AY150294; AAN73219.1; -; mRNA.
DR EMBL; AC011713; AAF14678.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36465.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60438.1; -; Genomic_DNA.
DR EMBL; AY085698; AAM62917.1; -; mRNA.
DR EMBL; AK221057; BAD94839.1; -; mRNA.
DR PIR; H96841; H96841.
DR RefSeq; NP_001322724.1; NM_001335020.1.
DR RefSeq; NP_565247.1; NM_106738.2.
DR AlphaFoldDB; Q9SAH0; -.
DR SMR; Q9SAH0; -.
DR BioGRID; 29648; 2.
DR IntAct; Q9SAH0; 2.
DR STRING; 3702.AT1G80900.1; -.
DR TCDB; 1.A.35.5.2; the cora metal ion transporter (mit) family.
DR iPTMnet; Q9SAH0; -.
DR PaxDb; Q9SAH0; -.
DR PRIDE; Q9SAH0; -.
DR ProteomicsDB; 238999; -.
DR EnsemblPlants; AT1G80900.1; AT1G80900.1; AT1G80900.
DR EnsemblPlants; AT1G80900.2; AT1G80900.2; AT1G80900.
DR GeneID; 844430; -.
DR Gramene; AT1G80900.1; AT1G80900.1; AT1G80900.
DR Gramene; AT1G80900.2; AT1G80900.2; AT1G80900.
DR KEGG; ath:AT1G80900; -.
DR Araport; AT1G80900; -.
DR TAIR; locus:2025767; AT1G80900.
DR eggNOG; KOG2662; Eukaryota.
DR HOGENOM; CLU_034694_1_1_1; -.
DR InParanoid; Q9SAH0; -.
DR OMA; QNSSPDY; -.
DR OrthoDB; 1363588at2759; -.
DR PhylomeDB; Q9SAH0; -.
DR PRO; PR:Q9SAH0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SAH0; baseline and differential.
DR Genevisible; Q9SAH0; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015693; P:magnesium ion transport; IBA:GO_Central.
DR CDD; cd12823; Mrs2_Mfm1p-like; 1.
DR InterPro; IPR045863; CorA_TM1_TM2.
DR InterPro; IPR002523; MgTranspt_CorA/ZnTranspt_ZntB.
DR InterPro; IPR039204; MRS2-like.
DR PANTHER; PTHR13890; PTHR13890; 1.
DR Pfam; PF01544; CorA; 1.
DR SUPFAM; SSF144083; SSF144083; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Ion transport; Magnesium; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..443
FT /note="Magnesium transporter MRS2-10"
FT /id="PRO_0000394174"
FT TRANSMEM 379..399
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 415..435
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 399..401
FT /note="Required for magnesium transport activity"
FT CONFLICT 120
FT /note="L -> S (in Ref. 5; BAD94839)"
FT /evidence="ECO:0000305"
FT CONFLICT 168
FT /note="L -> F (in Ref. 1; AAN73219 and 4; AAM62917)"
FT /evidence="ECO:0000305"
FT CONFLICT 434
FT /note="Y -> F (in Ref. 1; AAN73219 and 4; AAM62917)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 443 AA; 50463 MW; 4AEE36DE801616F2 CRC64;
MSELKERLLP PRPASAINLR GDAGSRPSPS GRQPLLGVDV LGLKKRGQGL KSWIRVDTSA
NSQVIEVDKF TMMRRCDLPA RDLRLLDPLF VYPSTILGRE KAIVVNLEQI RCIITADEVL
LLNSLDNYVL RYVVELQQRL KASSVTEVWN QDSLELSRRR SRSLDNVLQN SSPDYLPFEF
RALEVALEAA CTFLDSQASE LEIEAYPLLD ELTSKISTLN LERARRLKSR LVALTRRVQK
VRDEIEQLMD DDGDMAEMYL TEKKKRMEGS LYGDQSLPVY RTNDCFSLSA PVSPVSSPPE
SRRLEKSLSI VRSRHDSARS SEDATENIEE LEMLLEAYFV VIDSTLNKLT SLKEYIDDTE
DFINIQLDNV RNQLIQFELL LTTATFVVAI FGVVAGIFGM NFEIDFFEKP GAFKWVLAIT
GVCGLVVFLA FLWYYKRRRL MPL
