ID   MRS2A_ORYSJ             Reviewed;         474 AA.
AC   Q9AUK4; A0A0P0W1J9; Q10F20; Q10F21;
DT   18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Magnesium transporter MRS2-A, chloroplastic;
DE   Flags: Precursor;
GN   Name=MRS2-A; OrderedLocusNames=Os03g0684400, LOC_Os03g48000;
GN   ORFNames=OsJ_12139, OSJNBb0072E24.13;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [7]
RP   GENE FAMILY.
RX   PubMed=19966073; DOI=10.1105/tpc.109.070557;
RA   Gebert M., Meschenmoser K., Svidova S., Weghuber J., Schweyen R.,
RA   Eifler K., Lenz H., Weyand K., Knoop V.;
RT   "A root-expressed magnesium transporter of the MRS2/MGT gene family in
RT   Arabidopsis thaliana allows for growth in low-Mg2+ environments.";
RL   Plant Cell 21:4018-4030(2009).
CC   -!- FUNCTION: Magnesium transporter that may mediate the influx of
CC       magnesium in chloroplast. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid. Plastid, chloroplast membrane
CC       {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the CorA metal ion transporter (MIT) (TC
CC       1.A.35.5) family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABF98245.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AC087851; AAK14424.1; -; Genomic_DNA.
DR   EMBL; DP000009; ABF98244.1; -; Genomic_DNA.
DR   EMBL; DP000009; ABF98245.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP008209; BAF12833.1; -; Genomic_DNA.
DR   EMBL; AP014959; BAS85790.1; -; Genomic_DNA.
DR   EMBL; CM000140; EEE59710.1; -; Genomic_DNA.
DR   EMBL; AK100086; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_015633311.1; XM_015777825.1.
DR   AlphaFoldDB; Q9AUK4; -.
DR   SMR; Q9AUK4; -.
DR   STRING; 4530.OS03T0684400-01; -.
DR   PaxDb; Q9AUK4; -.
DR   PRIDE; Q9AUK4; -.
DR   EnsemblPlants; Os03t0684400-01; Os03t0684400-01; Os03g0684400.
DR   GeneID; 4333741; -.
DR   Gramene; Os03t0684400-01; Os03t0684400-01; Os03g0684400.
DR   KEGG; osa:4333741; -.
DR   eggNOG; KOG2662; Eukaryota.
DR   HOGENOM; CLU_025144_0_0_1; -.
DR   InParanoid; Q9AUK4; -.
DR   OMA; QVGAFCV; -.
DR   OrthoDB; 1363588at2759; -.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000007752; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   Genevisible; Q9AUK4; OS.
DR   GO; GO:0009941; C:chloroplast envelope; IBA:GO_Central.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015095; F:magnesium ion transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0010960; P:magnesium ion homeostasis; IEA:EnsemblPlants.
DR   GO; GO:0015693; P:magnesium ion transport; IBA:GO_Central.
DR   GO; GO:0010117; P:photoprotection; IEA:EnsemblPlants.
DR   GO; GO:0010027; P:thylakoid membrane organization; IEA:EnsemblPlants.
DR   CDD; cd12823; Mrs2_Mfm1p-like; 1.
DR   InterPro; IPR045863; CorA_TM1_TM2.
DR   InterPro; IPR002523; MgTranspt_CorA/ZnTranspt_ZntB.
DR   InterPro; IPR039204; MRS2-like.
DR   PANTHER; PTHR13890; PTHR13890; 1.
DR   Pfam; PF01544; CorA; 1.
DR   SUPFAM; SSF144083; SSF144083; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Ion transport; Magnesium; Membrane; Plastid;
KW   Reference proteome; Transit peptide; Transmembrane; Transmembrane helix;
KW   Transport.
FT   TRANSIT         1..55
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           56..474
FT                   /note="Magnesium transporter MRS2-A, chloroplastic"
FT                   /id="PRO_0000394265"
FT   TRANSMEM        412..432
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        444..464
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          79..129
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           432..434
FT                   /note="Required for magnesium transport activity"
FT   COMPBIAS        100..119
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        346
FT                   /note="M -> V (in Ref. 6; AK100086)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   474 AA;  51892 MW;  02213D1F79FEDF3B CRC64;
     MASVSSSPSY SSQAAVLLLL HQPPHQHGHG GACLRYRGSQ SQGRGNAVAT SLGLSAAGRG
     GAGGLLLLPP LPALRAAEGK DGRAVTKDEE EEAAAAAVEE EGEVEVRREE DKPGDDGSRE
     AAARGSGSGR FSADYISLGI REPVYEVIEV KSNGRMSTKK ISRRQLLKSS GLRLRDTRSV
     DPSLWLMNSM PSLLVREQAI LVNLGSLRAI AMHERVLIFN YNSPGGKAFL DSLLPRLNPR
     NINGGPAMPF QLEVVEAALL SRIQRLERRL MRIEPRVGAL LEVLPNRLTA DVLEQLRLSK
     QALVELGSRA GDLKQMLIDL LDDPHEIRRI CIMGRNCTLD KLSDNMECSV PLEKQIAEEE
     EEEIEMLLEN YLQRCESIHG QAERLLDSAR EMEDSIAVNL SSRRLEVSRV ELLLQVGTFC
     VAIGALIAGI FGMNLKSYLE TNAWAFWATT GGIVVGAVAG FFIMYSYLKT RKIL