MRS2B_ARATH
ID MRS2B_ARATH Reviewed; 459 AA.
AC Q058N4; Q9FFC4; Q9FPL0;
DT 18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT 14-NOV-2006, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Magnesium transporter MRS2-11, chloroplastic;
DE AltName: Full=Magnesium Transporter 10;
DE Short=AtMGT10;
DE Flags: Precursor;
GN Name=MRS2-11; Synonyms=GMN10, MGT10; OrderedLocusNames=At5g22830;
GN ORFNames=MRN17.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, GENE FAMILY, AND TISSUE SPECIFICITY.
RX PubMed=11752386; DOI=10.2307/3871533;
RA Li L., Tutone A.F., Drummond R.S., Gardner R.C., Luan S.;
RT "A novel family of magnesium transport genes in Arabidopsis.";
RL Plant Cell 13:2761-2775(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF Clones.";
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP SUBCELLULAR LOCATION, FUNCTION, TISSUE SPECIFICITY, AND INDUCTION BY LIGHT.
RX DOI=10.1016/j.plantsci.2005.08.018;
RA Drummond R.S., Tutone A.F., Li Y.-C., Gardner R.C.;
RT "A putative magnesium transporter AtMRS2-11 is localized to the plant
RT chloroplast envelope membrane system.";
RL Plant Sci. 170:78-89(2006).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=18431481; DOI=10.1371/journal.pone.0001994;
RA Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q.,
RA van Wijk K.J.;
RT "Sorting signals, N-terminal modifications and abundance of the chloroplast
RT proteome.";
RL PLoS ONE 3:E1994-E1994(2008).
RN [7]
RP GENE FAMILY, NOMENCLATURE, AND TISSUE SPECIFICITY.
RX PubMed=19966073; DOI=10.1105/tpc.109.070557;
RA Gebert M., Meschenmoser K., Svidova S., Weghuber J., Schweyen R.,
RA Eifler K., Lenz H., Weyand K., Knoop V.;
RT "A root-expressed magnesium transporter of the MRS2/MGT gene family in
RT Arabidopsis thaliana allows for growth in low-Mg2+ environments.";
RL Plant Cell 21:4018-4030(2009).
CC -!- FUNCTION: High-affinity magnesium transporter that mediates the influx
CC of magnesium in chloroplast. {ECO:0000269|PubMed:11752386,
CC ECO:0000269|Ref.5}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC {ECO:0000269|PubMed:18431481, ECO:0000269|Ref.5}; Multi-pass membrane
CC protein {ECO:0000269|PubMed:18431481, ECO:0000269|Ref.5}.
CC -!- TISSUE SPECIFICITY: Expressed in the green part of the plant.
CC Preferentially expressed in the spongy mesophyll cells and stomata of
CC young leaves but also detected in cotyledons and at the base of the
CC leaf petioles. {ECO:0000269|PubMed:11752386,
CC ECO:0000269|PubMed:19966073, ECO:0000269|Ref.5}.
CC -!- INDUCTION: By light. {ECO:0000269|Ref.5}.
CC -!- MISCELLANEOUS: Has the ability to complement a mutant in yeast lacking
CC magnesium transport capability.
CC -!- SIMILARITY: Belongs to the CorA metal ion transporter (MIT) (TC
CC 1.A.35.5) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10604.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF322255; AAG45213.1; -; mRNA.
DR EMBL; AB005243; BAB10604.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED93085.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM70562.1; -; Genomic_DNA.
DR EMBL; BT029184; ABJ17119.1; -; mRNA.
DR RefSeq; NP_001332159.1; NM_001343773.1.
DR RefSeq; NP_568424.1; NM_122188.3.
DR AlphaFoldDB; Q058N4; -.
DR SMR; Q058N4; -.
DR STRING; 3702.AT5G22830.1; -.
DR TCDB; 1.A.35.5.3; the cora metal ion transporter (mit) family.
DR PaxDb; Q058N4; -.
DR PRIDE; Q058N4; -.
DR ProteomicsDB; 250871; -.
DR EnsemblPlants; AT5G22830.1; AT5G22830.1; AT5G22830.
DR EnsemblPlants; AT5G22830.2; AT5G22830.2; AT5G22830.
DR GeneID; 832346; -.
DR Gramene; AT5G22830.1; AT5G22830.1; AT5G22830.
DR Gramene; AT5G22830.2; AT5G22830.2; AT5G22830.
DR KEGG; ath:AT5G22830; -.
DR Araport; AT5G22830; -.
DR TAIR; locus:2172641; AT5G22830.
DR eggNOG; KOG2662; Eukaryota.
DR HOGENOM; CLU_025144_0_0_1; -.
DR InParanoid; Q058N4; -.
DR OMA; CPRERSI; -.
DR OrthoDB; 1363588at2759; -.
DR PhylomeDB; Q058N4; -.
DR PRO; PR:Q058N4; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q058N4; baseline and differential.
DR Genevisible; Q058N4; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; IDA:TAIR.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; IMP:TAIR.
DR GO; GO:0010960; P:magnesium ion homeostasis; IMP:TAIR.
DR GO; GO:1903830; P:magnesium ion transmembrane transport; IMP:TAIR.
DR GO; GO:0015693; P:magnesium ion transport; IBA:GO_Central.
DR GO; GO:0010117; P:photoprotection; IMP:TAIR.
DR GO; GO:0010027; P:thylakoid membrane organization; IMP:TAIR.
DR CDD; cd12823; Mrs2_Mfm1p-like; 1.
DR InterPro; IPR002523; MgTranspt_CorA/ZnTranspt_ZntB.
DR InterPro; IPR039204; MRS2-like.
DR PANTHER; PTHR13890; PTHR13890; 1.
DR Pfam; PF01544; CorA; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Ion transport; Magnesium; Membrane; Plastid;
KW Reference proteome; Transit peptide; Transmembrane; Transmembrane helix;
KW Transport.
FT TRANSIT 1..62
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 63..459
FT /note="Magnesium transporter MRS2-11, chloroplastic"
FT /id="PRO_0000394175"
FT TRANSMEM 397..417
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 430..450
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 76..122
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 417..419
FT /note="Required for magnesium transport activity"
FT COMPBIAS 79..93
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 47..48
FT /note="RT -> KN (in Ref. 1; AAG45213)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 459 AA; 51093 MW; 2CC6E0B5906FF833 CRC64;
MALTPIPSTF TSLFNFSDHS PYPSPSLHYL LPGSSPSFSL QLSALSRTPI YFEALKVLSR
SKCFAKSPTT AEDFVGDYES LNVSDDDDGS DSNSSDGDNG GGRDDSKKID SSSSSSSSDS
TSLGIREPVY EVVEVKATGA ISTRKINRRQ LLKSSGLRPR DIRSVDPSLF MTNSVPSLLV
REHAILLNLG SLRAIAMRDR VLIFDYNRRG GRAFVDTLMP RLNPRSMNGG PSMPFELEAV
ESALISRIQR LEQRLMDIEP RVQALLEVLP NRLTADILEE LRISKQRLVE LGSRAGALRQ
MLLDLLEDPH EIRRICIMGR NCTLRRGDDD LECTLPSDKL IAEEEEEEIE MLLENYLQRC
ESCHGQAERL LDSAKEMEDS IAVNLSSRRL EVSRFELLLQ VGTFCVAVGA LIAGIFGMNL
RSYLEEQASA FWLTTGGIII GAAVAFFLMY SYLSRRKIF
