MRS2G_ORYSJ
ID MRS2G_ORYSJ Reviewed; 468 AA.
AC A3BV82; Q336V7; Q9AV41;
DT 18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 2.
DT 03-AUG-2022, entry version 74.
DE RecName: Full=Putative magnesium transporter MRS2-G;
GN Name=MRS2-G; OrderedLocusNames=Os10g0545000, LOC_Os10g39790;
GN ORFNames=OsJ_28078, OSJNBa0001O14.11;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12791992; DOI=10.1126/science.1083523;
RA Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S.,
RA Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D.,
RA Oates R., Palmer M., Pries G., Gibson J., Anderson H., Paradkar M.,
RA Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F.,
RA Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M.,
RA Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R.,
RA Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F.,
RA Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D.,
RA Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R.,
RA Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K.,
RA Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S.,
RA Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S.,
RA Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R.,
RA Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M.,
RA Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R.,
RA Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E.,
RA Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.;
RT "In-depth view of structure, activity, and evolution of rice chromosome
RT 10.";
RL Science 300:1566-1569(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP INTERACTION WITH CYCB2-2.
RX PubMed=14750518; DOI=10.1023/b:plan.0000007001.30865.0f;
RA Cooper B., Hutchison D., Park S., Guimil S., Luginbuehl P., Ellero C.,
RA Goff S.A., Glazebrook J.;
RT "Identification of rice (Oryza sativa) proteins linked to the cyclin-
RT mediated regulation of the cell cycle.";
RL Plant Mol. Biol. 53:273-279(2003).
RN [8]
RP GENE FAMILY.
RX PubMed=19966073; DOI=10.1105/tpc.109.070557;
RA Gebert M., Meschenmoser K., Svidova S., Weghuber J., Schweyen R.,
RA Eifler K., Lenz H., Weyand K., Knoop V.;
RT "A root-expressed magnesium transporter of the MRS2/MGT gene family in
RT Arabidopsis thaliana allows for growth in low-Mg2+ environments.";
RL Plant Cell 21:4018-4030(2009).
CC -!- FUNCTION: Putative magnesium transporter. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with CYCB2-2. {ECO:0000269|PubMed:14750518}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A3BV82-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A3BV82-2; Sequence=VSP_039233, VSP_039234;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to an intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the CorA metal ion transporter (MIT) (TC
CC 1.A.35.5) family. {ECO:0000305}.
CC -!- CAUTION: Lacks the GMN motif, which is a conserved feature of the
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK20062.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=EAZ43471.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC025783; AAK20062.1; ALT_INIT; Genomic_DNA.
DR EMBL; DP000086; ABB47950.1; -; Genomic_DNA.
DR EMBL; AP008216; BAF27118.1; -; Genomic_DNA.
DR EMBL; AP014966; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CM000145; EAZ43471.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AK069472; BAG91452.1; -; mRNA.
DR RefSeq; XP_015614208.1; XM_015758722.1. [A3BV82-1]
DR AlphaFoldDB; A3BV82; -.
DR SMR; A3BV82; -.
DR STRING; 4530.OS10T0545000-01; -.
DR PaxDb; A3BV82; -.
DR PRIDE; A3BV82; -.
DR GeneID; 4349276; -.
DR KEGG; osa:4349276; -.
DR eggNOG; KOG2662; Eukaryota.
DR InParanoid; A3BV82; -.
DR OrthoDB; 1363588at2759; -.
DR Proteomes; UP000000763; Chromosome 10.
DR Proteomes; UP000007752; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 10.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015693; P:magnesium ion transport; IBA:GO_Central.
DR CDD; cd12823; Mrs2_Mfm1p-like; 1.
DR InterPro; IPR039204; MRS2-like.
DR PANTHER; PTHR13890; PTHR13890; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Ion transport; Magnesium; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..468
FT /note="Putative magnesium transporter MRS2-G"
FT /id="PRO_0000394277"
FT TRANSMEM 402..422
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 437..457
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 183..204
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 12..43
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 44..68
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 187..202
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..125
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_039233"
FT VAR_SEQ 126..130
FT /note="SSSIL -> MFNCA (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_039234"
SQ SEQUENCE 468 AA; 51671 MW; 007DEFAD0E1C0B94 CRC64;
MGRRSGGRKL PFFASNASTS SSTKRTRSAR RLPSLTRPRA SSSPSPASPS PPPPSASHPA
PPSPPLAVSP AGAGKVGKKK AGARLWMRLD RWGVSETLHL DKGSIIRRAG LPPRDLRILG
PVFSDSSSIL AREKAMVINL EFIRAIVTAD EILLLDPLTI DVIPFVEQLT HHLPLKNLVC
GNGQPGGDDH GEKHDDSHGD QVPRLNEATG AEHELPFEFQ VLELALETVC SSFDVNVSGL
ERRATPVLEE LTKNVSTRNL DRVRTLKSDL TRLLAHVQKV RDEIEHLLDD NEDMAHLYLT
RKQLQNQQVE ALISSAASNS IVPGGTSLSR LNNSFRRSVS IATSMHLDND VEDLEMLLEA
YFMQLDGIRN RILSVREYID DTEDYVNIQL DNQRNELIQL QLTLTIASFG IAVNTFIAGA
FAMNIQSKLY SIDDGSFFWP FVGGTSSGCF MICIVLLWYA RWKKLLGP
