ID   ARN_BPT4                Reviewed;          92 AA.
AC   P39510;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Anti-restriction endonuclease;
DE   AltName: Full=Anti-rgl nuclease;
GN   Name=arn; Synonyms=asiA.1, motA.-6;
OS   Enterobacteria phage T4 (Bacteriophage T4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX   NCBI_TaxID=10665;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=T4ALC10;
RX   PubMed=9387160;
RA   Kim B.C., Kim K., Park E.H., Lim C.J.;
RT   "Nucleotide sequence and revised map location of the arn gene from
RT   bacteriophage T4.";
RL   Mol. Cells 7:694-696(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA   Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT   "Bacteriophage T4 genome.";
RL   Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN   [3]
RP   FUNCTION.
RX   PubMed=768783; DOI=10.1038/260454a0;
RA   Dharmalingam K., Goldberg E.B.;
RT   "Phage-coded protein prevents restriction of unmodified progeny T4 DNA.";
RL   Nature 260:454-456(1976).
RN   [4]
RP   FUNCTION.
RX   PubMed=380146; DOI=10.1016/0042-6822(79)90098-9;
RA   Dharmalingam K., Goldberg E.B.;
RT   "Restriction in vivo. IV. Effect of restriction of parental DNA on the
RT   expression of restriction alleviation systems in phage T4.";
RL   Virology 96:404-411(1979).
CC   -!- FUNCTION: Pays a role in the inhibition of the host restriction-
CC       modification system. Strongly inhibits the host mcrA endonuclease that
CC       cleaves 5-methyl and 5-hydroxymethylcytosines at the specific DNA
CC       sequence C(me)CGG. {ECO:0000269|PubMed:380146,
CC       ECO:0000269|PubMed:768783}.
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DR   EMBL; L37801; AAA86440.1; -; Genomic_DNA.
DR   EMBL; AF158101; AAD42538.1; -; Genomic_DNA.
DR   RefSeq; NP_049868.1; NC_000866.4.
DR   PDB; 3WX4; X-ray; 1.90 A; A=1-92.
DR   PDBsum; 3WX4; -.
DR   SMR; P39510; -.
DR   GeneID; 1258794; -.
DR   KEGG; vg:1258794; -.
DR   Proteomes; UP000009087; Genome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0099018; P:evasion by virus of host restriction-modification system; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   3D-structure; Endonuclease; Host-virus interaction; Hydrolase; Nuclease;
KW   Reference proteome; Restriction-modification system evasion by virus.
FT   CHAIN           1..92
FT                   /note="Anti-restriction endonuclease"
FT                   /id="PRO_0000164918"
FT   STRAND          11..14
FT                   /evidence="ECO:0007829|PDB:3WX4"
FT   HELIX           16..24
FT                   /evidence="ECO:0007829|PDB:3WX4"
FT   HELIX           26..43
FT                   /evidence="ECO:0007829|PDB:3WX4"
FT   STRAND          45..51
FT                   /evidence="ECO:0007829|PDB:3WX4"
FT   HELIX           55..57
FT                   /evidence="ECO:0007829|PDB:3WX4"
FT   STRAND          60..65
FT                   /evidence="ECO:0007829|PDB:3WX4"
FT   HELIX           71..84
FT                   /evidence="ECO:0007829|PDB:3WX4"
FT   HELIX           88..91
FT                   /evidence="ECO:0007829|PDB:3WX4"
SQ   SEQUENCE   92 AA;  10900 MW;  6CFE8EB1711C657D CRC64;
     MIIDSQSVVQ YTFKIDILEK LYKFLPNLYH SIVNELVEEL HLENNDFLIG TYKDLSKAGY
     FYVIPAPGKN IDDVLKTIMI YVHDYEIEDY FE