ID   MRS2_PLABA              Reviewed;         433 AA.
AC   A0A509AK04;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   18-SEP-2019, sequence version 1.
DT   25-MAY-2022, entry version 12.
DE   RecName: Full=Mitochondrial inner membrane magnesium transporter MIT1 {ECO:0000305};
DE   AltName: Full=PfMIT1 {ECO:0000303|PubMed:26796412};
DE   Flags: Precursor;
GN   Name=MIT1 {ECO:0000303|PubMed:26796412};
GN   ORFNames=PBANKA_0927900 {ECO:0000312|EMBL:VUC55819.1};
OS   Plasmodium berghei (strain Anka).
OC   Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC   Plasmodiidae; Plasmodium; Plasmodium (Vinckeia).
OX   NCBI_TaxID=5823 {ECO:0000312|Proteomes:UP000074855};
RN   [1] {ECO:0000312|Proteomes:UP000074855}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ANKA {ECO:0000312|Proteomes:UP000074855};
RX   PubMed=25359557; DOI=10.1186/s12915-014-0086-0;
RA   Otto T.D., Bohme U., Jackson A.P., Hunt M., Franke-Fayard B.,
RA   Hoeijmakers W.A., Religa A.A., Robertson L., Sanders M., Ogun S.A.,
RA   Cunningham D., Erhart A., Billker O., Khan S.M., Stunnenberg H.G.,
RA   Langhorne J., Holder A.A., Waters A.P., Newbold C.I., Pain A., Berriman M.,
RA   Janse C.J.;
RT   "A comprehensive evaluation of rodent malaria parasite genomes and gene
RT   expression.";
RL   BMC Biol. 12:86-86(2014).
RN   [2] {ECO:0000305}
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=26796412; DOI=10.1038/ncomms10519;
RA   Kenthirapalan S., Waters A.P., Matuschewski K., Kooij T.W.;
RT   "Functional profiles of orphan membrane transporters in the life cycle of
RT   the malaria parasite.";
RL   Nat. Commun. 7:10519-10519(2016).
CC   -!- FUNCTION: Mitochondrial inner membrane magnesium transporter required
CC       for mitochondrial magnesium homeostasis (By similarity). Involved in
CC       the development of the sporozoite in the mosquito vector midgut
CC       (PubMed:26796412). {ECO:0000250|UniProtKB:Q01926,
CC       ECO:0000269|PubMed:26796412}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000255|RuleBase:RU366042}; Multi-pass membrane protein
CC       {ECO:0000255|RuleBase:RU366042}.
CC   -!- DISRUPTION PHENOTYPE: Severe decrease in the number of sporozoites in
CC       the mosquito salivary gland (PubMed:26796412). Normal asexual stage in
CC       mouse erythrocytes and normal exflagellation of male gametocytes
CC       (PubMed:26796412). {ECO:0000269|PubMed:26796412}.
CC   -!- SIMILARITY: Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35)
CC       family. {ECO:0000255|RuleBase:RU366042}.
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DR   EMBL; LK023124; VUC55819.1; -; Genomic_DNA.
DR   STRING; 5821.PBANKA_092790; -.
DR   VEuPathDB; PlasmoDB:PBANKA_0927900; -.
DR   OMA; NNHNPTL; -.
DR   Proteomes; UP000074855; Chromosome 9.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   CDD; cd12823; Mrs2_Mfm1p-like; 1.
DR   InterPro; IPR045863; CorA_TM1_TM2.
DR   InterPro; IPR039204; MRS2-like.
DR   PANTHER; PTHR13890; PTHR13890; 1.
DR   SUPFAM; SSF144083; SSF144083; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Ion transport; Magnesium; Membrane; Mitochondrion;
KW   Mitochondrion inner membrane; Reference proteome; Transit peptide;
KW   Transmembrane; Transmembrane helix; Transport.
FT   TRANSIT         1..?
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..433
FT                   /note="Mitochondrial inner membrane magnesium transporter
FT                   MIT1"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000455041"
FT   TOPO_DOM        ?..359
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        360..380
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        381..396
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        397..417
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        418..433
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000305"
FT   COILED          257..298
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   433 AA;  51225 MW;  8F415135CA9F33BF CRC64;
     MFRWLNINKC MSKIIHPYVL KPNNCIHTNW LFKFATLKNE NKIFPKKNLN NNILMQNIKV
     ADDGNAICEQ LLFSKYDLPY LLKIPVSDLR LIDTGNNNHN PTILIRKDVI LLRTGFISCI
     IRYNETWLFE GSNSVVINAK DLISKNLKKQ NNNKFKNCNN DEIVESLCRK RNCTDNGKEN
     MKQINNDEKE ELNYLNIINN FYRYNKGKAY FEFLCLDICM QLSIKEYEND LEGINYKIRD
     IILLQRKEEN NELNMLTNKL LRDMMKIKNN LQKLSNLLNA LRTNIEKILN NENDMKNMYL
     TYLNKNPYNN LKDCSDLEIL LETHLQLTDE LYGQLENVEE KITHYEELMR LNLDYNRNKF
     ILLNAKISFS TLLFSISSVV TSLFGMNLKN FVEDSNYAFI IVSIFVSVWS IIGIYVTKNI
     NTLLKFFDRY NFR