MRS4_YEAST
ID MRS4_YEAST Reviewed; 304 AA.
AC P23500; D6VXB3;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1991, sequence version 1.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Mitochondrial RNA-splicing protein MRS4;
GN Name=MRS4; OrderedLocusNames=YKR052C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M1301;
RX PubMed=1703236; DOI=10.1016/0022-2836(91)90608-9;
RA Wiesenberger G., Link T.A., von Ahsen U., Waldherr M., Schweyen R.J.;
RT "MRS3 and MRS4, two suppressors of mtRNA splicing defects in yeast, are new
RT members of the mitochondrial carrier family.";
RL J. Mol. Biol. 217:23-37(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: MRS4 suppresses a mitochondrial splice defect in the first
CC intron of the COB gene. It may act as a carrier, exerting its
CC suppressor activity via modulation of solute concentrations in the
CC mitochondrion (possibly of cations). Not essential.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane
CC protein.
CC -!- MISCELLANEOUS: Present with 1340 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
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DR EMBL; X56444; CAA39828.1; -; Genomic_DNA.
DR EMBL; Z28277; CAA82130.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09203.1; -; Genomic_DNA.
DR PIR; S13533; S13533.
DR RefSeq; NP_012978.1; NM_001179842.1.
DR AlphaFoldDB; P23500; -.
DR SMR; P23500; -.
DR BioGRID; 34183; 80.
DR DIP; DIP-4751N; -.
DR IntAct; P23500; 23.
DR STRING; 4932.YKR052C; -.
DR TCDB; 2.A.29.5.2; the mitochondrial carrier (mc) family.
DR MaxQB; P23500; -.
DR PaxDb; P23500; -.
DR PRIDE; P23500; -.
DR EnsemblFungi; YKR052C_mRNA; YKR052C; YKR052C.
DR GeneID; 853926; -.
DR KEGG; sce:YKR052C; -.
DR SGD; S000001760; MRS4.
DR VEuPathDB; FungiDB:YKR052C; -.
DR eggNOG; KOG0760; Eukaryota.
DR GeneTree; ENSGT00940000169345; -.
DR HOGENOM; CLU_015166_3_1_1; -.
DR InParanoid; P23500; -.
DR OMA; KVMNPSQ; -.
DR BioCyc; YEAST:G3O-32022-MON; -.
DR PRO; PR:P23500; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P23500; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:SGD.
DR GO; GO:0031966; C:mitochondrial membrane; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0015093; F:ferrous iron transmembrane transporter activity; EXP:Reactome.
DR GO; GO:0005381; F:iron ion transmembrane transporter activity; IMP:SGD.
DR GO; GO:0048250; P:iron import into the mitochondrion; IDA:SGD.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 1.50.40.10; -; 2.
DR InterPro; IPR002067; Mit_carrier.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR Pfam; PF00153; Mito_carr; 3.
DR PRINTS; PR00926; MITOCARRIER.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; mRNA processing;
KW Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..304
FT /note="Mitochondrial RNA-splicing protein MRS4"
FT /id="PRO_0000090692"
FT TRANSMEM 23..41
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..102
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 120..139
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..194
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..228
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 275..288
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 21..108
FT /note="Solcar 1"
FT REPEAT 118..200
FT /note="Solcar 2"
FT REPEAT 207..300
FT /note="Solcar 3"
SQ SEQUENCE 304 AA; 33307 MW; 5ABBF9858B547EDC CRC64;
MNTSELSIAE EIDYEALPSH APLHSQLLAG AFAGIMEHSL MFPIDALKTR VQAAGLNKAA
STGMISQISK ISTMEGSMAL WKGVQSVILG AGPAHAVYFG TYEFCKARLI SPEDMQTHQP
MKTALSGTIA TIAADALMNP FDTVKQRLQL DTNLRVWNVT KQIYQNEGFA AFYYSYPTTL
AMNIPFAAFN FMIYESASKF FNPQNSYNPL IHCLCGGISG ATCAALTTPL DCIKTVLQVR
GSETVSIEIM KDANTFGRAS RAILEVHGWK GFWRGLKPRI VANIPATAIS WTAYECAKHF
LMKN
