MRX12_YEAST
ID MRX12_YEAST Reviewed; 519 AA.
AC P47084; D6VWH7;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=MIOREX complex component 12 {ECO:0000305|PubMed:25683707};
DE AltName: Full=Mitochondrial organization of gene expression protein 12 {ECO:0000303|PubMed:25683707};
DE Flags: Precursor;
GN Name=MRX12 {ECO:0000303|PubMed:25683707};
GN OrderedLocusNames=YJR003C {ECO:0000312|SGD:S000003763};
GN ORFNames=J1415, YJR83.30;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP IDENTIFICATION OF PROBABLE INITIATION SITE.
RX PubMed=12748633; DOI=10.1038/nature01644;
RA Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.;
RT "Sequencing and comparison of yeast species to identify genes and
RT regulatory elements.";
RL Nature 423:241-254(2003).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP IDENTIFICATION OF PROBABLE INITIATION SITE.
RX PubMed=12775844; DOI=10.1126/science.1084337;
RA Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J.,
RA Waterston R., Cohen B.A., Johnston M.;
RT "Finding functional features in Saccharomyces genomes by phylogenetic
RT footprinting.";
RL Science 301:71-76(2003).
RN [7]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=16823961; DOI=10.1021/pr050477f;
RA Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.;
RT "Toward the complete yeast mitochondrial proteome: multidimensional
RT separation techniques for mitochondrial proteomics.";
RL J. Proteome Res. 5:1543-1554(2006).
RN [8]
RP FUNCTION, AND SUBUNIT.
RX PubMed=25683707; DOI=10.1016/j.celrep.2015.01.012;
RA Kehrein K., Schilling R., Moller-Hergt B.V., Wurm C.A., Jakobs S.,
RA Lamkemeyer T., Langer T., Ott M.;
RT "Organization of mitochondrial gene expression in two distinct ribosome-
RT containing assemblies.";
RL Cell Rep. 10:843-853(2015).
CC -!- FUNCTION: Component of MIOREX complexes, large expressome-like
CC assemblies of ribosomes with factors involved in all the steps of post-
CC transcriptional gene expression. {ECO:0000269|PubMed:25683707}.
CC -!- SUBUNIT: Associates with the mitochondrial ribosome.
CC {ECO:0000269|PubMed:25683707}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14562095,
CC ECO:0000269|PubMed:16823961}.
CC -!- MISCELLANEOUS: Present with 3280 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA60924.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAA89525.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X87611; CAA60924.1; ALT_INIT; Genomic_DNA.
DR EMBL; Z49503; CAA89525.1; ALT_INIT; Genomic_DNA.
DR EMBL; BK006943; DAA08793.1; -; Genomic_DNA.
DR PIR; S55190; S55190.
DR RefSeq; NP_012536.2; NM_001181660.1.
DR AlphaFoldDB; P47084; -.
DR BioGRID; 33759; 60.
DR DIP; DIP-4242N; -.
DR IntAct; P47084; 6.
DR STRING; 4932.YJR003C; -.
DR MaxQB; P47084; -.
DR PaxDb; P47084; -.
DR PRIDE; P47084; -.
DR EnsemblFungi; YJR003C_mRNA; YJR003C; YJR003C.
DR GeneID; 853459; -.
DR KEGG; sce:YJR003C; -.
DR SGD; S000003763; MRX12.
DR VEuPathDB; FungiDB:YJR003C; -.
DR eggNOG; ENOG502QVKH; Eukaryota.
DR HOGENOM; CLU_038968_0_0_1; -.
DR InParanoid; P47084; -.
DR OMA; NDYIQNV; -.
DR BioCyc; YEAST:G3O-31649-MON; -.
DR PRO; PR:P47084; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P47084; protein.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
PE 1: Evidence at protein level;
KW Mitochondrion; Reference proteome; Transit peptide.
FT TRANSIT 1..35
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 36..519
FT /note="MIOREX complex component 12"
FT /id="PRO_0000203081"
SQ SEQUENCE 519 AA; 59766 MW; 99A19168D63DE347 CRC64;
MLRSLHSAAT LSNKRFYSLI SHSNRKNIIK KLLRHPSFDP IRHHLPEDIT TIDPYSLSQN
VIESLNKLEV PKKDAAMVHN MMIENLSDLD YGVATIHSNN LRDLDLKPSL PAIKQIIRNN
PGRVQSSWEL FTQYKASMEN VPDELMEVVL EKIIKFDKAE KVDGKKSLTY QDLVRCLYLI
NHFSSNYNLP SELVEPILIY IVDNGIPNVL GSVLKYKIPL SFFDKYVSEM TQYQICELYD
FYSLDNIVAD PLVLHKCLTV LGENEKIQQT EEEKEIISKL EEEIDIVKSQ CHDNWSLEFP
NWSVRKTATS FEELFLEIQK RNIDKKDFEL AHKLLRLIGA FKGKVSLFFK LYDEYLLKFK
NNEDDLMFEA FLTLCCQGYK SSNEKMLQYA EAFIKEDFDS KLESKIQSVL IVANAKANID
LSLKIYNSNI STAKREKDKY TDLAESDVLT ESLILAFLSR DDADFARVIF DGALGEKLIS
GPTAAKKIKN LLAQYGEALE TKTSKQVMQT KIEHYMESI
