MS18A_MOUSE
ID MS18A_MOUSE Reviewed; 204 AA.
AC Q9CZJ6;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Protein Mis18-alpha;
GN Name=Mis18a;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Egg;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Required for recruitment of CENPA to centromeres and normal
CC chromosome segregation during mitosis. {ECO:0000250|UniProtKB:Q9NYP9}.
CC -!- SUBUNIT: Homodimer, and heterodimer with OIP5/MIS18B. Identified in a
CC complex containing MIS18A, OIP5/MIS18B, MIS18BP1, RBBP7 and RBBP4.
CC {ECO:0000250|UniProtKB:Q9NYP9}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9NYP9}.
CC Chromosome {ECO:0000250|UniProtKB:Q9NYP9}. Chromosome, centromere
CC {ECO:0000250|UniProtKB:Q9NYP9}. Note=Associated with centromeres in
CC interphase cells, from late anaphase to the G1 phase. Not detected on
CC centromeres during earlier phases of mitosis. Associated with
CC chromatin. {ECO:0000250|UniProtKB:Q9NYP9}.
CC -!- SIMILARITY: Belongs to the mis18 family. {ECO:0000255|PROSITE-
CC ProRule:PRU01129}.
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DR EMBL; AK012533; BAB28302.1; -; mRNA.
DR EMBL; AK163310; BAE37292.1; -; mRNA.
DR EMBL; BC079900; AAH79900.1; -; mRNA.
DR CCDS; CCDS28316.1; -.
DR RefSeq; NP_079918.1; NM_025642.1.
DR AlphaFoldDB; Q9CZJ6; -.
DR SMR; Q9CZJ6; -.
DR ComplexPortal; CPX-3273; Mis18 complex.
DR STRING; 10090.ENSMUSP00000097150; -.
DR iPTMnet; Q9CZJ6; -.
DR PhosphoSitePlus; Q9CZJ6; -.
DR EPD; Q9CZJ6; -.
DR MaxQB; Q9CZJ6; -.
DR PaxDb; Q9CZJ6; -.
DR PRIDE; Q9CZJ6; -.
DR ProteomicsDB; 291352; -.
DR Antibodypedia; 6843; 63 antibodies from 18 providers.
DR Ensembl; ENSMUST00000099554; ENSMUSP00000097150; ENSMUSG00000022978.
DR GeneID; 66578; -.
DR KEGG; mmu:66578; -.
DR UCSC; uc007zwj.1; mouse.
DR CTD; 54069; -.
DR MGI; MGI:1913828; Mis18a.
DR VEuPathDB; HostDB:ENSMUSG00000022978; -.
DR eggNOG; ENOG502S3DZ; Eukaryota.
DR GeneTree; ENSGT00940000154267; -.
DR HOGENOM; CLU_101031_0_0_1; -.
DR InParanoid; Q9CZJ6; -.
DR OMA; EMKMLVM; -.
DR OrthoDB; 1453138at2759; -.
DR PhylomeDB; Q9CZJ6; -.
DR TreeFam; TF333200; -.
DR Reactome; R-MMU-606279; Deposition of new CENPA-containing nucleosomes at the centromere.
DR BioGRID-ORCS; 66578; 26 hits in 76 CRISPR screens.
DR ChiTaRS; Mis18a; mouse.
DR PRO; PR:Q9CZJ6; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q9CZJ6; protein.
DR Bgee; ENSMUSG00000022978; Expressed in paneth cell and 256 other tissues.
DR Genevisible; Q9CZJ6; MM.
DR GO; GO:0000785; C:chromatin; IBA:GO_Central.
DR GO; GO:0000775; C:chromosome, centromeric region; IDA:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0034080; P:CENP-A containing chromatin assembly; IMP:MGI.
DR GO; GO:0007059; P:chromosome segregation; IMP:MGI.
DR GO; GO:0044030; P:regulation of DNA methylation; IMP:MGI.
DR InterPro; IPR034752; Mis18.
DR InterPro; IPR004910; Yippee/Mis18/Cereblon.
DR Pfam; PF03226; Yippee-Mis18; 1.
DR PROSITE; PS51793; MIS18; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Centromere; Chromosome; Isopeptide bond;
KW Metal-binding; Mitosis; Nucleus; Phosphoprotein; Reference proteome;
KW Ubl conjugation; Zinc.
FT CHAIN 1..204
FT /note="Protein Mis18-alpha"
FT /id="PRO_0000278473"
FT DOMAIN 51..149
FT /note="Mis18"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01129"
FT BINDING 56
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01129"
FT BINDING 59
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01129"
FT BINDING 112
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01129"
FT BINDING 115
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01129"
FT MOD_RES 13
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NYP9"
FT MOD_RES 16
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NYP9"
FT MOD_RES 17
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NYP9"
FT MOD_RES 204
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NYP9"
FT CROSSLNK 133
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q9NYP9"
SQ SEQUENCE 204 AA; 22949 MW; BBD667250FDF9B0F CRC64;
MSSESPLLEK RLSEDSSRYL RLQKWANMSS ADALGLEKER PEEKAAAAEN PLVFLCARCR
RPLGDSLTWV ASQEDTNCIL LRSVSCNVSV DKEPKLSKCR DEDGCILEAL YCTGCSLSLG
YVYRCTPKNL DYKRDLFCLS VEAVESYTLG SSEKQIVSED KELFNLESRV EIEKSIKQME
EVLTALQKKL REVESKLSLA SRGS
