ID   MS18A_PLEMO             Reviewed;         233 AA.
AC   B1MT51;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   03-AUG-2022, entry version 31.
DE   RecName: Full=Protein Mis18-alpha;
GN   Name=MIS18A;
OS   Plecturocebus moloch (Dusky titi monkey) (Callicebus moloch).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Platyrrhini;
OC   Pitheciidae; Callicebinae; Plecturocebus.
OX   NCBI_TaxID=9523;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Antonellis A., Ayele K., Benjamin B., Blakesley R.W., Boakye A.,
RA   Bouffard G.G., Brinkley C., Brooks S., Chu G., Coleman H., Engle J.,
RA   Gestole M., Greene A., Guan X., Gupta J., Haghighi P., Han J., Hansen N.,
RA   Ho S.-L., Hu P., Hunter G., Hurle B., Idol J.R., Kwong P., Laric P.,
RA   Larson S., Lee-Lin S.-Q., Legaspi R., Madden M., Maduro Q.L., Maduro V.B.,
RA   Margulies E.H., Masiello C., Maskeri B., McDowell J., Mojidi H.A.,
RA   Mullikin J.C., Oestreicher J.S., Park M., Portnoy M.E., Prasad A., Puri O.,
RA   Reddix-Dugue N., Schandler K., Schueler M.G., Sison C., Stantripop S.,
RA   Stephen E., Taye A., Thomas J.W., Thomas P.J., Tsipouri V., Ung L.,
RA   Vogt J.L., Wetherby K.D., Young A., Green E.D.;
RT   "NISC comparative sequencing initiative.";
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for recruitment of CENPA to centromeres and normal
CC       chromosome segregation during mitosis. {ECO:0000250|UniProtKB:Q9NYP9}.
CC   -!- SUBUNIT: Homodimer, and heterodimer with OIP5/MIS18B. Identified in a
CC       complex containing MIS18A, OIP5/MIS18B, MIS18BP1, RBBP7 and RBBP4.
CC       {ECO:0000250|UniProtKB:Q9NYP9}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9NYP9}.
CC       Chromosome {ECO:0000250|UniProtKB:Q9NYP9}. Chromosome, centromere
CC       {ECO:0000250|UniProtKB:Q9NYP9}. Note=Associated with centromeres in
CC       interphase cells, from late anaphase to the G1 phase. Not detected on
CC       centromeres during earlier phases of mitosis. Associated with
CC       chromatin. {ECO:0000250|UniProtKB:Q9NYP9}.
CC   -!- SIMILARITY: Belongs to the mis18 family. {ECO:0000255|PROSITE-
CC       ProRule:PRU01129}.
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DR   EMBL; DP000619; ACA51046.1; -; Genomic_DNA.
DR   AlphaFoldDB; B1MT51; -.
DR   SMR; B1MT51; -.
DR   GO; GO:0000775; C:chromosome, centromeric region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   InterPro; IPR034752; Mis18.
DR   InterPro; IPR004910; Yippee/Mis18/Cereblon.
DR   Pfam; PF03226; Yippee-Mis18; 1.
DR   PROSITE; PS51793; MIS18; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Centromere; Chromosome; Isopeptide bond;
KW   Metal-binding; Mitosis; Nucleus; Phosphoprotein; Ubl conjugation; Zinc.
FT   CHAIN           1..233
FT                   /note="Protein Mis18-alpha"
FT                   /id="PRO_0000359880"
FT   DOMAIN          80..178
FT                   /note="Mis18"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01129"
FT   BINDING         85
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01129"
FT   BINDING         88
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01129"
FT   BINDING         141
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01129"
FT   BINDING         144
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01129"
FT   MOD_RES         36
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NYP9"
FT   MOD_RES         39
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NYP9"
FT   MOD_RES         40
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NYP9"
FT   MOD_RES         233
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NYP9"
FT   CROSSLNK        162
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO2)"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NYP9"
SQ   SEQUENCE   233 AA;  26175 MW;  6457B95A5F72F95D CRC64;
     MAGALSLRCR TGCTGGCEYG HKGKCRDSSL LSKRLSEDSS RHQLLQKWTS MWRSTSEDAS
     VADTEKARLE EAAAAAEERP LVFLCSGCRR PLGDSLSWVT SQEDTNCILL RCVSCNVSVD
     KEQKLSKREK ENGCILETLH CTGCSLNLGY VYRCTPKNLD YKRDLFCLNV EAVESYVLGS
     SEKQIVSEDK ELFNLESRVE IEKSLKQMED VLQALQMKLW EVESKLSFAT CKS