MS18A_RAT
ID MS18A_RAT Reviewed; 223 AA.
AC B2RZC4;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-2008, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=Protein Mis18-alpha;
GN Name=Mis18a;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Required for recruitment of CENPA to centromeres and normal
CC chromosome segregation during mitosis. {ECO:0000250|UniProtKB:Q9NYP9}.
CC -!- SUBUNIT: Homodimer, and heterodimer with OIP5/MIS18B. Identified in a
CC complex containing MIS18A, OIP5/MIS18B, MIS18BP1, RBBP7 and RBBP4.
CC {ECO:0000250|UniProtKB:Q9NYP9}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9NYP9}.
CC Chromosome {ECO:0000250|UniProtKB:Q9NYP9}. Chromosome, centromere
CC {ECO:0000250|UniProtKB:Q9NYP9}. Note=Associated with centromeres in
CC interphase cells, from late anaphase to the G1 phase. Not detected on
CC centromeres during earlier phases of mitosis. Associated with
CC chromatin. {ECO:0000250|UniProtKB:Q9NYP9}.
CC -!- SIMILARITY: Belongs to the mis18 family. {ECO:0000255|PROSITE-
CC ProRule:PRU01129}.
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DR EMBL; BC167102; AAI67102.1; -; mRNA.
DR RefSeq; NP_001120995.2; NM_001127523.2.
DR AlphaFoldDB; B2RZC4; -.
DR STRING; 10116.ENSRNOP00000037080; -.
DR PhosphoSitePlus; B2RZC4; -.
DR PaxDb; B2RZC4; -.
DR Ensembl; ENSRNOT00000037444; ENSRNOP00000037080; ENSRNOG00000021555.
DR GeneID; 288272; -.
DR KEGG; rno:288272; -.
DR UCSC; RGD:1310778; rat.
DR CTD; 54069; -.
DR RGD; 1310778; Mis18a.
DR eggNOG; ENOG502S3DZ; Eukaryota.
DR GeneTree; ENSGT00940000154267; -.
DR HOGENOM; CLU_101031_0_0_1; -.
DR InParanoid; B2RZC4; -.
DR OrthoDB; 1453138at2759; -.
DR PhylomeDB; B2RZC4; -.
DR TreeFam; TF333200; -.
DR Reactome; R-RNO-606279; Deposition of new CENPA-containing nucleosomes at the centromere.
DR PRO; PR:B2RZC4; -.
DR Proteomes; UP000002494; Chromosome 11.
DR Genevisible; B2RZC4; RN.
DR GO; GO:0000785; C:chromatin; IBA:GO_Central.
DR GO; GO:0000775; C:chromosome, centromeric region; ISO:RGD.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; ISO:RGD.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0034080; P:CENP-A containing chromatin assembly; ISO:RGD.
DR GO; GO:0007059; P:chromosome segregation; ISO:RGD.
DR GO; GO:0044030; P:regulation of DNA methylation; ISO:RGD.
DR InterPro; IPR034752; Mis18.
DR InterPro; IPR004910; Yippee/Mis18/Cereblon.
DR Pfam; PF03226; Yippee-Mis18; 1.
DR PROSITE; PS51793; MIS18; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Centromere; Chromosome; Isopeptide bond;
KW Metal-binding; Mitosis; Nucleus; Phosphoprotein; Reference proteome;
KW Ubl conjugation; Zinc.
FT CHAIN 1..223
FT /note="Protein Mis18-alpha"
FT /id="PRO_0000359882"
FT DOMAIN 71..169
FT /note="Mis18"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01129"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..22
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 76
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01129"
FT BINDING 79
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01129"
FT BINDING 132
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01129"
FT BINDING 135
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01129"
FT MOD_RES 33
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NYP9"
FT MOD_RES 36
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NYP9"
FT MOD_RES 37
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NYP9"
FT MOD_RES 223
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NYP9"
FT CROSSLNK 153
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q9NYP9"
SQ SEQUENCE 223 AA; 25216 MW; D7CFCC75AEFF990E CRC64;
MAGTFSLEPC STSSSCNHQG KRSESSLLEK RLSEDSSRHW RLQKWASMSS ADASRTLLER
REEKAAAAEN PLVFLCTRCR RPLGDSLTWV ASQEDTNCIL LRSVSSNVSV DKEQKLSKCR
DEDGCILETL YCSGCSLSLG YVYRCTPKNL DYKRNLFCLS VEAVESYTLG SSEQQIVSEE
EFFNLESRVE IEKSIKQMED VLTVMQAKLW EVESKLSKAG RYS
