MS18B_MOUSE
ID MS18B_MOUSE Reviewed; 241 AA.
AC A2AQ14; Q14AW4; Q14BH1;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Protein Mis18-beta;
DE AltName: Full=Opa-interacting protein 5 homolog;
GN Name=Oip5; Synonyms=Ms18b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Required for recruitment of CENPA to centromeres and normal
CC chromosome segregation during mitosis. {ECO:0000250|UniProtKB:O43482}.
CC -!- SUBUNIT: Homodimer, and heterodimer with MIS18A. Identified in a
CC complex containing MIS18A, OIP5/MIS18B, MIS18BP1, RBBP7 and RBBP4.
CC {ECO:0000250|UniProtKB:O43482}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O43482}.
CC Chromosome {ECO:0000250|UniProtKB:O43482}. Chromosome, centromere
CC {ECO:0000250|UniProtKB:O43482}. Note=Associated with centromeres in
CC interphase cells, from late anaphase to the G1 phase. Not detected on
CC centromeres during earlier phases of mitosis. Associated with
CC chromatin. {ECO:0000250|UniProtKB:O43482}.
CC -!- SIMILARITY: Belongs to the mis18 family. {ECO:0000255|PROSITE-
CC ProRule:PRU01129}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAI16661.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AL844536; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC115883; AAI15884.1; -; mRNA.
DR EMBL; BC116660; AAI16661.1; ALT_INIT; mRNA.
DR CCDS; CCDS38205.1; -.
DR RefSeq; NP_001036118.1; NM_001042653.1.
DR AlphaFoldDB; A2AQ14; -.
DR SMR; A2AQ14; -.
DR ComplexPortal; CPX-3273; Mis18 complex.
DR STRING; 10090.ENSMUSP00000115219; -.
DR iPTMnet; A2AQ14; -.
DR PhosphoSitePlus; A2AQ14; -.
DR jPOST; A2AQ14; -.
DR MaxQB; A2AQ14; -.
DR PaxDb; A2AQ14; -.
DR PRIDE; A2AQ14; -.
DR ProteomicsDB; 290061; -.
DR Antibodypedia; 23263; 139 antibodies from 20 providers.
DR Ensembl; ENSMUST00000123818; ENSMUSP00000115219; ENSMUSG00000072980.
DR GeneID; 70645; -.
DR KEGG; mmu:70645; -.
DR UCSC; uc008ltz.1; mouse.
DR CTD; 11339; -.
DR MGI; MGI:1917895; Oip5.
DR VEuPathDB; HostDB:ENSMUSG00000072980; -.
DR eggNOG; ENOG502S5R3; Eukaryota.
DR GeneTree; ENSGT00940000154267; -.
DR HOGENOM; CLU_098875_0_0_1; -.
DR InParanoid; A2AQ14; -.
DR OMA; KEVTPDQ; -.
DR OrthoDB; 1253924at2759; -.
DR PhylomeDB; A2AQ14; -.
DR TreeFam; TF330739; -.
DR Reactome; R-MMU-606279; Deposition of new CENPA-containing nucleosomes at the centromere.
DR BioGRID-ORCS; 70645; 31 hits in 70 CRISPR screens.
DR PRO; PR:A2AQ14; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; A2AQ14; protein.
DR Bgee; ENSMUSG00000072980; Expressed in manus and 141 other tissues.
DR Genevisible; A2AQ14; MM.
DR GO; GO:0015030; C:Cajal body; ISO:MGI.
DR GO; GO:0000785; C:chromatin; ISO:MGI.
DR GO; GO:0010369; C:chromocenter; IDA:MGI.
DR GO; GO:0000775; C:chromosome, centromeric region; IBA:GO_Central.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0016607; C:nuclear speck; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0034080; P:CENP-A containing chromatin assembly; IBA:GO_Central.
DR GO; GO:0007059; P:chromosome segregation; IBA:GO_Central.
DR InterPro; IPR034752; Mis18.
DR InterPro; IPR004910; Yippee/Mis18/Cereblon.
DR Pfam; PF03226; Yippee-Mis18; 1.
DR PROSITE; PS51793; MIS18; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Centromere; Chromosome; Metal-binding; Mitosis;
KW Nucleus; Phosphoprotein; Reference proteome; Zinc.
FT CHAIN 1..241
FT /note="Protein Mis18-beta"
FT /id="PRO_0000359883"
FT DOMAIN 69..168
FT /note="Mis18"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01129"
FT BINDING 74
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01129"
FT BINDING 77
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01129"
FT BINDING 131
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01129"
FT BINDING 134
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01129"
FT MOD_RES 14
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O43482"
FT MOD_RES 42
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O43482"
FT CONFLICT 17
FT /note="R -> H (in Ref. 2; AAI15884/AAI16661)"
FT /evidence="ECO:0000305"
FT CONFLICT 45
FT /note="G -> V (in Ref. 2; AAI16661)"
FT /evidence="ECO:0000305"
FT CONFLICT 219
FT /note="F -> S (in Ref. 2; AAI15884/AAI16661)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 241 AA; 26861 MW; 63AC2693958E29F4 CRC64;
MATLSRRSLR FLETQSRRDS CDTPFSLTSS MEWDTQVVEG FSPLGSSESK VKASPVDLRL
PAWLEPERCA VFHCARCYAV LGDTLHLAWD LSRSLGALAF SKVTNNVVLL EPFLVGIEGF
LKSSTYNLLF CNSCGTPVGF HLYSTHAAMA ALRGHFCLSS DKMLCYLLKT NAIVNTSEMD
FHNVPLPEKI AELKEKIMLM HTRLNSLTGL LKGKSPHQFK QENQQARKQH ILGLTASPKI
L
