MSC1_YEAST
ID MSC1_YEAST Reviewed; 513 AA.
AC Q03104; D6W0F7;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Meiotic sister chromatid recombination protein 1;
GN Name=MSC1; OrderedLocusNames=YML128C; ORFNames=YM4987.07C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP PROTEIN SEQUENCE OF 49-53; 171-180 AND 241-250.
RC STRAIN=ATCC 201238 / W303-1B;
RX PubMed=11502169; DOI=10.1021/bi010277r;
RA Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S.,
RA Schmitter J.-M.;
RT "Yeast mitochondrial dehydrogenases are associated in a supramolecular
RT complex.";
RL Biochemistry 40:9758-9769(2001).
RN [5]
RP MUTANT ANALYSIS.
RX PubMed=10511544; DOI=10.1093/genetics/153.2.621;
RA Thompson D.A., Stahl F.W.;
RT "Genetic control of recombination partner preference in yeast meiosis.
RT Isolation and characterization of mutants elevated for meiotic unequal
RT sister-chromatid recombination.";
RL Genetics 153:621-641(1999).
RN [6]
RP ERRATUM OF PUBMED:10511544.
RA Thompson D.A., Stahl F.W.;
RL Genetics 164:1241-1241(2003).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-237 AND SER-243, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 76625 / YPH499;
RX PubMed=17761666; DOI=10.1074/mcp.m700098-mcp200;
RA Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B.,
RA van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.;
RT "Profiling phosphoproteins of yeast mitochondria reveals a role of
RT phosphorylation in assembly of the ATP synthase.";
RL Mol. Cell. Proteomics 6:1896-1906(2007).
CC -!- MISCELLANEOUS: Originally MSC1 was identified in a screen for mutants
CC that show an increase in meiotic unequal sister-chromatid recombination
CC (SCR).
CC -!- MISCELLANEOUS: Present with 1070 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z50178; CAA90555.1; -; Genomic_DNA.
DR EMBL; AY693046; AAT93065.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09771.1; -; Genomic_DNA.
DR PIR; S58200; S58200.
DR RefSeq; NP_013578.1; NM_001182491.1.
DR AlphaFoldDB; Q03104; -.
DR SMR; Q03104; -.
DR BioGRID; 35077; 612.
DR STRING; 4932.YML128C; -.
DR iPTMnet; Q03104; -.
DR UCD-2DPAGE; Q03104; -.
DR MaxQB; Q03104; -.
DR PaxDb; Q03104; -.
DR PRIDE; Q03104; -.
DR EnsemblFungi; YML128C_mRNA; YML128C; YML128C.
DR GeneID; 854911; -.
DR KEGG; sce:YML128C; -.
DR SGD; S000004597; MSC1.
DR VEuPathDB; FungiDB:YML128C; -.
DR eggNOG; ENOG502RNIV; Eukaryota.
DR HOGENOM; CLU_028751_0_0_1; -.
DR InParanoid; Q03104; -.
DR OMA; FNAWSDS; -.
DR BioCyc; YEAST:G3O-32706-MON; -.
DR PRO; PR:Q03104; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q03104; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0005739; C:mitochondrion; IDA:SGD.
DR GO; GO:0005886; C:plasma membrane; HDA:SGD.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:SGD.
DR InterPro; IPR018803; Ish1/Msc1-like.
DR Pfam; PF10281; Ish1; 4.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Phosphoprotein; Reference proteome.
FT CHAIN 1..513
FT /note="Meiotic sister chromatid recombination protein 1"
FT /id="PRO_0000096591"
FT MOD_RES 237
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17761666"
FT MOD_RES 243
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17761666"
FT CONFLICT 52
FT /note="T -> I (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 513 AA; 59589 MW; FFD0BD1CB35205A4 CRC64;
MKQFKLVNAV SASFVLIGLV LANSDSVFDK WTQEDLADYL RDNKKSLEKY ATDSIEDLKT
EASQVWDKHA QPKPWWQVWS SDSSSVSNSN PGWFGYTGSS DHPVSDWLFD TWSTDSLRNF
LKKNGVDVDD AKASKDSLVK TAKENFNKIS KSLKSSGYYP SSSYFDSWST KDLQNWLNDN
GIDYDKAVQS KDELVQKVKE NIYRTSEKAE QQRLGLLESL DLAHQQILDT SGQIKDTVFD
KWSSDQLTNW LESHKVNIDK NMAKKHDYLV RMAKENSANL KDDIYWYLDY MKRESSPFLT
KTPEYVGSVW DSSKNFLTNL YSKFRGKTDN VINDTFLVGL DSWPKDKLKM FLDARGIKYS
MLSTEHQLRE LVKKSRNEKL KILPKDYQKY FDNSNWSLDD IKGWFADKKD DFQDSQTYST
IMQDFDKVSK NTNDAKDQIA KTWSNTFQSW SQEDLLQYLK SFGVPVKQTS TKDDLINLAK
QNTQWLFGTV KEPAYKRYLH NVKNWSKSIL GFN
