MSC3_YEAST
ID MSC3_YEAST Reviewed; 728 AA.
AC Q05812; D6VYL9; Q7LIF4;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Meiotic sister-chromatid recombination protein 3;
GN Name=MSC3; OrderedLocusNames=YLR219W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION.
RX PubMed=10511544; DOI=10.1093/genetics/153.2.621;
RA Thompson D.A., Stahl F.W.;
RT "Genetic control of recombination partner preference in yeast meiosis.
RT Isolation and characterization of mutants elevated for meiotic unequal
RT sister-chromatid recombination.";
RL Genetics 153:621-641(1999).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151; SER-155 AND SER-363, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-57; SER-64; SER-127; SER-151;
RP SER-155; THR-646 AND SER-660, AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: May be involved in the control of meiotic sister-chromatid
CC recombination. {ECO:0000269|PubMed:10511544}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:14562095};
CC Peripheral membrane protein {ECO:0000269|PubMed:14562095}. Note=Cell
CC periphery.
CC -!- MISCELLANEOUS: Present with 131 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; U19027; AAB67420.1; -; Genomic_DNA.
DR EMBL; U14913; AAB67423.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09535.1; -; Genomic_DNA.
DR PIR; S48569; S48569.
DR RefSeq; NP_013320.1; NM_001182106.1.
DR AlphaFoldDB; Q05812; -.
DR BioGRID; 31486; 87.
DR DIP; DIP-6321N; -.
DR IntAct; Q05812; 8.
DR MINT; Q05812; -.
DR STRING; 4932.YLR219W; -.
DR iPTMnet; Q05812; -.
DR MaxQB; Q05812; -.
DR PaxDb; Q05812; -.
DR PRIDE; Q05812; -.
DR TopDownProteomics; Q05812; -.
DR EnsemblFungi; YLR219W_mRNA; YLR219W; YLR219W.
DR GeneID; 850916; -.
DR KEGG; sce:YLR219W; -.
DR SGD; S000004209; MSC3.
DR VEuPathDB; FungiDB:YLR219W; -.
DR eggNOG; ENOG502RZHH; Eukaryota.
DR HOGENOM; CLU_380449_0_0_1; -.
DR InParanoid; Q05812; -.
DR OMA; INGYEYV; -.
DR BioCyc; YEAST:G3O-32333-MON; -.
DR PRO; PR:Q05812; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q05812; protein.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:SGD.
PE 1: Evidence at protein level;
KW Cell membrane; DNA recombination; Meiosis; Membrane; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..728
FT /note="Meiotic sister-chromatid recombination protein 3"
FT /id="PRO_0000096595"
FT REGION 33..171
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 236..261
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 300..335
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 363..403
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 421..454
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 495..514
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 561..728
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 33..107
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 119..171
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 307..324
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 495..510
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 575..712
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 57
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 64
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 127
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 151
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:19779198"
FT MOD_RES 155
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:19779198"
FT MOD_RES 363
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT MOD_RES 646
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 660
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
SQ SEQUENCE 728 AA; 80530 MW; 1962D638DB602B71 CRC64;
MVFGFTKRDR RVPDLSRYDY YYQNHEDYNK SPQLSAAAAS AASAASPDRT NYSRSHSLVS
HAPSIPRQRS SVKSPGRRLS TSSAAPPTSR AAAKQYSQKT YSLRSQRSGE YHLHPPGYTT
NGSRMNSMTS GANVRRNYGK NKSTAGNNND SRANSITVKT TQVTDPSGRT QSITKKTIRK
INGYEYVETT TTTKNLVPLG DSQRHFDEFS ENYMLQDDDI LEEQASDNIH DIIEENETDN
EKPYSPVSES HLQDDSELNV EKPDFPLGSY FHHKYSTDVM PLEEESSLSN FSDALDYIPP
THQTSSKYIH NKRKQASTTR RKKRPPAVKN AEAEAKKPLT EAEMYLKALE VAKRNVYHTD
AASDNASAPL GSNKSRKSRM GQKMTLRSSS DSPTATANLV KSNVEVQPKR FTSSFFSRNT
KSAPHEVHNH SVSTHFKSNK AVDPVPEPKS ANTGLTDKEM YDQALKIAQA RYYNSHGIQP
EAVDNSTTAA KPRQVGVSHL GSTGSIPPNE QHYLGDSEIP VQSEVHEYEP IPLQKTKTTG
SSKNKFKTMF DKVLQFSQEN YGYQHKKEQG EQTPVTRNAE ESFPAASISE GVTTAKPSSN
EGVMTNPVVT DSPSPLQQQI DSTTASSNGQ SQGNVPTSAV ASTTRTRSPE LQDNLKSSSS
LLQDQTPQRQ EDATDPTTSS TNELSAAEPT MVTSTHATKT IQAQTQDPPT KHKKSSFFTK
LFKKKSSR
