MSCL_BACSU
ID MSCL_BACSU Reviewed; 130 AA.
AC P94585;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Large-conductance mechanosensitive channel {ECO:0000255|HAMAP-Rule:MF_00115};
GN Name=mscL {ECO:0000255|HAMAP-Rule:MF_00115}; Synonyms=ywpC;
GN OrderedLocusNames=BSU36360;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9353933; DOI=10.1099/00221287-143-10-3313;
RA Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V.,
RA Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G.,
RA Kunst F., Danchin A., Glaser P.;
RT "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334
RT degrees).";
RL Microbiology 143:3313-3328(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=9632260; DOI=10.1046/j.1365-2958.1998.00821.x;
RA Moe P.C., Blount P., Kung C.;
RT "Functional and structural conservation in the mechanosensitive channel
RT mscL implicates elements crucial for mechanosensation.";
RL Mol. Microbiol. 28:583-592(1998).
CC -!- FUNCTION: Channel that opens in response to stretch forces in the
CC membrane lipid bilayer. Forms a nonselective ion channel with a
CC conductance of about 4 nanosiemens. May participate in the regulation
CC of osmotic pressure changes within the cell.
CC {ECO:0000269|PubMed:9632260}.
CC -!- SUBUNIT: Homopentamer. {ECO:0000250|UniProtKB:P0A742,
CC ECO:0000255|HAMAP-Rule:MF_00115}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00115,
CC ECO:0000269|PubMed:9632260}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:P0A742, ECO:0000255|HAMAP-Rule:MF_00115}.
CC -!- SIMILARITY: Belongs to the MscL family. {ECO:0000255|HAMAP-
CC Rule:MF_00115, ECO:0000305}.
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DR EMBL; Z83337; CAB05944.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15653.1; -; Genomic_DNA.
DR PIR; E70065; E70065.
DR RefSeq; NP_391517.1; NC_000964.3.
DR RefSeq; WP_003242893.1; NZ_JNCM01000034.1.
DR AlphaFoldDB; P94585; -.
DR STRING; 224308.BSU36360; -.
DR TCDB; 1.A.22.1.3; the large conductance mechanosensitive ion channel (mscl) family.
DR PaxDb; P94585; -.
DR PRIDE; P94585; -.
DR EnsemblBacteria; CAB15653; CAB15653; BSU_36360.
DR GeneID; 936909; -.
DR KEGG; bsu:BSU36360; -.
DR PATRIC; fig|224308.43.peg.3811; -.
DR eggNOG; COG1970; Bacteria.
DR InParanoid; P94585; -.
DR OMA; AWIIFLM; -.
DR PhylomeDB; P94585; -.
DR BioCyc; BSUB:BSU36360-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0008381; F:mechanosensitive ion channel activity; IBA:GO_Central.
DR GO; GO:0009992; P:cellular water homeostasis; IBA:GO_Central.
DR GO; GO:0006811; P:ion transport; IBA:GO_Central.
DR Gene3D; 1.10.1200.120; -; 1.
DR HAMAP; MF_00115; MscL; 1.
DR InterPro; IPR019823; Mechanosensitive_channel_CS.
DR InterPro; IPR001185; MS_channel.
DR InterPro; IPR037673; MSC/AndL.
DR InterPro; IPR036019; MscL_channel.
DR PANTHER; PTHR30266; PTHR30266; 1.
DR Pfam; PF01741; MscL; 1.
DR PRINTS; PR01264; MECHCHANNEL.
DR SUPFAM; SSF81330; SSF81330; 1.
DR TIGRFAMs; TIGR00220; mscL; 1.
DR PROSITE; PS01327; MSCL; 1.
PE 3: Inferred from homology;
KW Cell membrane; Ion channel; Ion transport; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..130
FT /note="Large-conductance mechanosensitive channel"
FT /id="PRO_0000192433"
FT TOPO_DOM 1..14
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P9WJN5"
FT TRANSMEM 15..43
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P9WJN5"
FT TOPO_DOM 44..65
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:P9WJN5"
FT TRANSMEM 66..85
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P9WJN5"
FT TOPO_DOM 86..130
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P9WJN5"
SQ SEQUENCE 130 AA; 14280 MW; 0941C01C2AAA8A9A CRC64;
MWNEFKAFAM RGNIVDLAIG VVIGGAFGKI VTSLVNDIIM PLVGLLLGGL DFSGLSFTFG
DAVVKYGSFI QTIVNFLIIS FSIFIVIRTL NGLRRKKEAE EEAAEEAVDA QEELLKEIRD
LLKQQAKSPE
