ID   MSCL_CLOPE              Reviewed;         152 AA.
AC   P53380; P54747;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   31-JAN-2002, sequence version 2.
DT   25-MAY-2022, entry version 130.
DE   RecName: Full=Large-conductance mechanosensitive channel {ECO:0000255|HAMAP-Rule:MF_00115};
GN   Name=mscL {ECO:0000255|HAMAP-Rule:MF_00115}; OrderedLocusNames=CPE0174;
OS   Clostridium perfringens (strain 13 / Type A).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=195102;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 3628 / NCIMB 10662 / Type C;
RX   PubMed=7489908; DOI=10.1016/0378-1119(95)00490-w;
RA   Matsushita O., Jung C.M., Okabe A.;
RT   "Identification of the gene encoding a mechanosensitive channel MscL
RT   homologue in Clostridium perfringens.";
RL   Gene 165:147-148(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=13 / Type A;
RX   PubMed=11792842; DOI=10.1073/pnas.022493799;
RA   Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T.,
RA   Ogasawara N., Hattori M., Kuhara S., Hayashi H.;
RT   "Complete genome sequence of Clostridium perfringens, an anaerobic flesh-
RT   eater.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002).
CC   -!- FUNCTION: Channel that opens in response to stretch forces in the
CC       membrane lipid bilayer. May participate in the regulation of osmotic
CC       pressure changes within the cell. {ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SUBUNIT: Homopentamer. {ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00115};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SIMILARITY: Belongs to the MscL family. {ECO:0000255|HAMAP-
CC       Rule:MF_00115, ECO:0000305}.
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DR   EMBL; D50309; BAA08847.1; -; Genomic_DNA.
DR   EMBL; BA000016; BAB79880.1; -; Genomic_DNA.
DR   RefSeq; WP_011009654.1; NC_003366.1.
DR   AlphaFoldDB; P53380; -.
DR   SMR; P53380; -.
DR   STRING; 195102.gene:10489418; -.
DR   EnsemblBacteria; BAB79880; BAB79880; BAB79880.
DR   KEGG; cpe:CPE0174; -.
DR   HOGENOM; CLU_095787_2_3_9; -.
DR   OMA; AWIIFLM; -.
DR   Proteomes; UP000000818; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0008381; F:mechanosensitive ion channel activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.1200.120; -; 1.
DR   HAMAP; MF_00115; MscL; 1.
DR   InterPro; IPR019823; Mechanosensitive_channel_CS.
DR   InterPro; IPR001185; MS_channel.
DR   InterPro; IPR037673; MSC/AndL.
DR   InterPro; IPR036019; MscL_channel.
DR   PANTHER; PTHR30266; PTHR30266; 1.
DR   Pfam; PF01741; MscL; 1.
DR   PRINTS; PR01264; MECHCHANNEL.
DR   SUPFAM; SSF81330; SSF81330; 1.
DR   TIGRFAMs; TIGR00220; mscL; 1.
DR   PROSITE; PS01327; MSCL; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Ion channel; Ion transport; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..152
FT                   /note="Large-conductance mechanosensitive channel"
FT                   /id="PRO_0000192438"
FT   TRANSMEM        14..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00115"
FT   TRANSMEM        81..101
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00115"
FT   CONFLICT        22
FT                   /note="I -> V (in Ref. 1; BAA08847)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        43
FT                   /note="I -> V (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        45
FT                   /note="R -> S (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        100
FT                   /note="A -> V (in Ref. 1; BAA08847)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        118
FT                   /note="Q -> K (in Ref. 1; BAA08847)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        130
FT                   /note="C -> Y (in Ref. 1; BAA08847)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   152 AA;  16811 MW;  528E9236BF45C87F CRC64;
     MWKEFKEFAM KGNVIDLAIG VIIGGAFGKI VTSLVNDIIM PVIGRLVGKV DFSNLYINLS
     GQQFNSLQEA QAAGAATINY GLFLNNLINF LIIAFSIFIA IKQINKLKNF TKKKEEVQVE
     ATEKDCPYCC TKIDIKATRC PHCTSVLEEA TN