ID   MSCL_HAEIN              Reviewed;         128 AA.
AC   P44789;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Large-conductance mechanosensitive channel {ECO:0000255|HAMAP-Rule:MF_00115};
GN   Name=mscL {ECO:0000255|HAMAP-Rule:MF_00115}; OrderedLocusNames=HI_0626;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA   Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA   Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA   Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA   Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
RN   [2]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=9632260; DOI=10.1046/j.1365-2958.1998.00821.x;
RA   Moe P.C., Blount P., Kung C.;
RT   "Functional and structural conservation in the mechanosensitive channel
RT   mscL implicates elements crucial for mechanosensation.";
RL   Mol. Microbiol. 28:583-592(1998).
CC   -!- FUNCTION: Channel that opens in response to stretch forces in the
CC       membrane lipid bilayer. Forms a nonselective ion channel with a
CC       conductance of about 4 nanosiemens. May participate in the regulation
CC       of osmotic pressure changes within the cell.
CC       {ECO:0000269|PubMed:9632260}.
CC   -!- SUBUNIT: Homopentamer. {ECO:0000250|UniProtKB:P0A742,
CC       ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00115, ECO:0000269|PubMed:9632260}; Multi-pass membrane protein
CC       {ECO:0000250|UniProtKB:P0A742, ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SIMILARITY: Belongs to the MscL family. {ECO:0000255|HAMAP-
CC       Rule:MF_00115, ECO:0000305}.
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DR   EMBL; L42023; AAC22286.1; -; Genomic_DNA.
DR   PIR; G64155; G64155.
DR   RefSeq; NP_438786.1; NC_000907.1.
DR   RefSeq; WP_005658680.1; NC_000907.1.
DR   AlphaFoldDB; P44789; -.
DR   SMR; P44789; -.
DR   STRING; 71421.HI_0626; -.
DR   EnsemblBacteria; AAC22286; AAC22286; HI_0626.
DR   KEGG; hin:HI_0626; -.
DR   PATRIC; fig|71421.8.peg.652; -.
DR   eggNOG; COG1970; Bacteria.
DR   HOGENOM; CLU_095787_0_0_6; -.
DR   OMA; AWIIFLM; -.
DR   PhylomeDB; P44789; -.
DR   BioCyc; HINF71421:G1GJ1-653-MON; -.
DR   Proteomes; UP000000579; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0008381; F:mechanosensitive ion channel activity; IBA:GO_Central.
DR   GO; GO:0009992; P:cellular water homeostasis; IBA:GO_Central.
DR   GO; GO:0006811; P:ion transport; IBA:GO_Central.
DR   Gene3D; 1.10.1200.120; -; 1.
DR   HAMAP; MF_00115; MscL; 1.
DR   InterPro; IPR019823; Mechanosensitive_channel_CS.
DR   InterPro; IPR001185; MS_channel.
DR   InterPro; IPR037673; MSC/AndL.
DR   InterPro; IPR036019; MscL_channel.
DR   PANTHER; PTHR30266; PTHR30266; 1.
DR   Pfam; PF01741; MscL; 1.
DR   PRINTS; PR01264; MECHCHANNEL.
DR   SUPFAM; SSF81330; SSF81330; 1.
DR   TIGRFAMs; TIGR00220; mscL; 1.
DR   PROSITE; PS01327; MSCL; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Ion channel; Ion transport; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..128
FT                   /note="Large-conductance mechanosensitive channel"
FT                   /id="PRO_0000192444"
FT   TOPO_DOM        1..16
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P9WJN5"
FT   TRANSMEM        17..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P9WJN5"
FT   TOPO_DOM        46..74
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P9WJN5"
FT   TRANSMEM        75..94
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P9WJN5"
FT   TOPO_DOM        95..128
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P9WJN5"
SQ   SEQUENCE   128 AA;  14198 MW;  BD3A68D27200E51A CRC64;
     MNFIKEFREF AMRGNVVDMA VGVIIGSAFG KIVSSLVSDI FTPVLGILTG GIDFKDMKFV
     LAQAQGDVPA VTLNYGLFIQ NVIDFIIIAF AIFMMIKVIN KVRKPEEKKT APKAETLLTE
     IRDLLKNK