ID   MSCL_LACH4              Reviewed;         125 AA.
AC   A8YTR2;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Large-conductance mechanosensitive channel {ECO:0000255|HAMAP-Rule:MF_00115};
GN   Name=mscL {ECO:0000255|HAMAP-Rule:MF_00115}; OrderedLocusNames=lhv_0445;
OS   Lactobacillus helveticus (strain DPC 4571).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=405566;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DPC 4571;
RX   PubMed=17993529; DOI=10.1128/jb.01295-07;
RA   Callanan M., Kaleta P., O'Callaghan J., O'Sullivan O., Jordan K.,
RA   McAuliffe O., Sangrador-Vegas A., Slattery L., Fitzgerald G.F.,
RA   Beresford T., Ross R.P.;
RT   "Genome sequence of Lactobacillus helveticus: an organism distinguished by
RT   selective gene loss and IS element expansion.";
RL   J. Bacteriol. 190:727-735(2008).
CC   -!- FUNCTION: Channel that opens in response to stretch forces in the
CC       membrane lipid bilayer. May participate in the regulation of osmotic
CC       pressure changes within the cell. {ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SUBUNIT: Homopentamer. {ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00115};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SIMILARITY: Belongs to the MscL family. {ECO:0000255|HAMAP-
CC       Rule:MF_00115}.
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DR   EMBL; CP000517; ABX26653.1; -; Genomic_DNA.
DR   RefSeq; WP_012211456.1; NC_010080.1.
DR   AlphaFoldDB; A8YTR2; -.
DR   STRING; 405566.lhv_0445; -.
DR   EnsemblBacteria; ABX26653; ABX26653; lhv_0445.
DR   KEGG; lhe:lhv_0445; -.
DR   eggNOG; COG1970; Bacteria.
DR   HOGENOM; CLU_095787_0_0_9; -.
DR   OMA; AWIIFLM; -.
DR   Proteomes; UP000000790; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0008381; F:mechanosensitive ion channel activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.1200.120; -; 1.
DR   HAMAP; MF_00115; MscL; 1.
DR   InterPro; IPR019823; Mechanosensitive_channel_CS.
DR   InterPro; IPR001185; MS_channel.
DR   InterPro; IPR037673; MSC/AndL.
DR   InterPro; IPR036019; MscL_channel.
DR   PANTHER; PTHR30266; PTHR30266; 1.
DR   Pfam; PF01741; MscL; 1.
DR   PRINTS; PR01264; MECHCHANNEL.
DR   SUPFAM; SSF81330; SSF81330; 1.
DR   TIGRFAMs; TIGR00220; mscL; 1.
DR   PROSITE; PS01327; MSCL; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Ion channel; Ion transport; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..125
FT                   /note="Large-conductance mechanosensitive channel"
FT                   /id="PRO_1000071347"
FT   TRANSMEM        14..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00115"
FT   TRANSMEM        67..87
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00115"
SQ   SEQUENCE   125 AA;  14031 MW;  4F1737DA1E402AD4 CRC64;
     MLKEFKQFIA RGNVIDLAVG VIIGAAFTAI VQSLVKNLIN PLIGLFIGRI DLSNLTLKVG
     EANFRYGSFL NSIINFLIIS FIVFLIVKAV NKFTKKEEEE TPAAPTETDY LKEIRDLLKE
     KRSIK