ID   MSCL_LACLA              Reviewed;         122 AA.
AC   Q9CDW0;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 104.
DE   RecName: Full=Large-conductance mechanosensitive channel {ECO:0000255|HAMAP-Rule:MF_00115};
GN   Name=mscL {ECO:0000255|HAMAP-Rule:MF_00115}; OrderedLocusNames=LL2101;
GN   ORFNames=L153973;
OS   Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=272623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IL1403;
RX   PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA   Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA   Ehrlich S.D., Sorokin A.;
RT   "The complete genome sequence of the lactic acid bacterium Lactococcus
RT   lactis ssp. lactis IL1403.";
RL   Genome Res. 11:731-753(2001).
CC   -!- FUNCTION: Channel that opens in response to stretch forces in the
CC       membrane lipid bilayer. May participate in the regulation of osmotic
CC       pressure changes within the cell. {ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SUBUNIT: Homopentamer. {ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00115};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SIMILARITY: Belongs to the MscL family. {ECO:0000255|HAMAP-
CC       Rule:MF_00115}.
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DR   EMBL; AE005176; AAK06199.1; -; Genomic_DNA.
DR   PIR; E86887; E86887.
DR   RefSeq; NP_268258.1; NC_002662.1.
DR   RefSeq; WP_010906303.1; NC_002662.1.
DR   AlphaFoldDB; Q9CDW0; -.
DR   STRING; 272623.L153973; -.
DR   PaxDb; Q9CDW0; -.
DR   EnsemblBacteria; AAK06199; AAK06199; L153973.
DR   GeneID; 60356895; -.
DR   GeneID; 66443095; -.
DR   KEGG; lla:L153973; -.
DR   PATRIC; fig|272623.7.peg.2260; -.
DR   eggNOG; COG1970; Bacteria.
DR   HOGENOM; CLU_095787_0_0_9; -.
DR   OMA; AWIIFLM; -.
DR   Proteomes; UP000002196; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0008381; F:mechanosensitive ion channel activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.1200.120; -; 1.
DR   HAMAP; MF_00115; MscL; 1.
DR   InterPro; IPR019823; Mechanosensitive_channel_CS.
DR   InterPro; IPR001185; MS_channel.
DR   InterPro; IPR037673; MSC/AndL.
DR   InterPro; IPR036019; MscL_channel.
DR   PANTHER; PTHR30266; PTHR30266; 1.
DR   Pfam; PF01741; MscL; 1.
DR   PRINTS; PR01264; MECHCHANNEL.
DR   SUPFAM; SSF81330; SSF81330; 1.
DR   TIGRFAMs; TIGR00220; mscL; 1.
DR   PROSITE; PS01327; MSCL; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Ion channel; Ion transport; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..122
FT                   /note="Large-conductance mechanosensitive channel"
FT                   /id="PRO_0000192445"
FT   TRANSMEM        14..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00115"
FT   TRANSMEM        67..87
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00115"
SQ   SEQUENCE   122 AA;  13758 MW;  CEA799C9981B9FA2 CRC64;
     MLKEFKNFIL RGNVLDLAVG VIIGAAFTAL VKSLVDNLIN PLIGMFVQST ALAHLSVTVG
     KTKFTYGAFL NDVINFIITA FVIFILIKFI NKLFPKKEET VEEQKNEELE TLQEIRDLLK
     KQ