ID   MSCL_MYCTA              Reviewed;         151 AA.
AC   A5U127;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Large-conductance mechanosensitive channel {ECO:0000255|HAMAP-Rule:MF_00115};
GN   Name=mscL {ECO:0000255|HAMAP-Rule:MF_00115}; OrderedLocusNames=MRA_0992;
OS   Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=419947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25177 / H37Ra;
RX   PubMed=18584054; DOI=10.1371/journal.pone.0002375;
RA   Zheng H., Lu L., Wang B., Pu S., Zhang X., Zhu G., Shi W., Zhang L.,
RA   Wang H., Wang S., Zhao G., Zhang Y.;
RT   "Genetic basis of virulence attenuation revealed by comparative genomic
RT   analysis of Mycobacterium tuberculosis strain H37Ra versus H37Rv.";
RL   PLoS ONE 3:E2375-E2375(2008).
CC   -!- FUNCTION: Channel that opens in response to stretch forces in the
CC       membrane lipid bilayer. The force required to trigger channel opening
CC       depends on the nature of the membrane lipids; the presence of
CC       phosphatidylinositol enhances mechanosensitivity of the channel. May
CC       participate in the regulation of osmotic pressure changes within the
CC       cell. {ECO:0000250|UniProtKB:P9WJN5}.
CC   -!- SUBUNIT: Homopentamer. {ECO:0000250|UniProtKB:P9WJN5,
CC       ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P9WJN5,
CC       ECO:0000255|HAMAP-Rule:MF_00115}; Multi-pass membrane protein
CC       {ECO:0000250|UniProtKB:P9WJN5, ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SIMILARITY: Belongs to the MscL family. {ECO:0000255|HAMAP-
CC       Rule:MF_00115, ECO:0000305}.
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DR   EMBL; CP000611; ABQ72727.1; -; Genomic_DNA.
DR   RefSeq; WP_003405128.1; NZ_CP016972.1.
DR   AlphaFoldDB; A5U127; -.
DR   SMR; A5U127; -.
DR   STRING; 419947.MRA_0992; -.
DR   EnsemblBacteria; ABQ72727; ABQ72727; MRA_0992.
DR   KEGG; mra:MRA_0992; -.
DR   eggNOG; COG1970; Bacteria.
DR   HOGENOM; CLU_095787_1_1_11; -.
DR   OMA; AWIIFLM; -.
DR   OrthoDB; 1863650at2; -.
DR   EvolutionaryTrace; A5U127; -.
DR   Proteomes; UP000001988; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0008381; F:mechanosensitive ion channel activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.1200.120; -; 1.
DR   HAMAP; MF_00115; MscL; 1.
DR   InterPro; IPR019823; Mechanosensitive_channel_CS.
DR   InterPro; IPR001185; MS_channel.
DR   InterPro; IPR037673; MSC/AndL.
DR   InterPro; IPR036019; MscL_channel.
DR   PANTHER; PTHR30266; PTHR30266; 1.
DR   Pfam; PF01741; MscL; 1.
DR   PRINTS; PR01264; MECHCHANNEL.
DR   SUPFAM; SSF81330; SSF81330; 1.
DR   TIGRFAMs; TIGR00220; mscL; 1.
DR   PROSITE; PS01327; MSCL; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Ion channel; Ion transport; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..151
FT                   /note="Large-conductance mechanosensitive channel"
FT                   /id="PRO_0000300062"
FT   TOPO_DOM        1..14
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P9WJN5"
FT   TRANSMEM        15..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P9WJN5"
FT   TOPO_DOM        44..68
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250|UniProtKB:P9WJN5"
FT   TRANSMEM        69..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P9WJN5"
FT   TOPO_DOM        90..151
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P9WJN5"
FT   REGION          122..151
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   151 AA;  16023 MW;  D682D3F2C1651E9C CRC64;
     MLKGFKEFLA RGNIVDLAVA VVIGTAFTAL VTKFTDSIIT PLINRIGVNA QSDVGILRIG
     IGGGQTIDLN VLLSAAINFF LIAFAVYFLV VLPYNTLRKK GEVEQPGDTQ VVLLTEIRDL
     LAQTNGDSPG RHGGRGTPSP TDGPRASTES Q