ID   MSCL_PSEFL              Reviewed;         136 AA.
AC   O68286;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Large-conductance mechanosensitive channel {ECO:0000255|HAMAP-Rule:MF_00115};
GN   Name=mscL {ECO:0000255|HAMAP-Rule:MF_00115};
OS   Pseudomonas fluorescens.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=294;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=9632260; DOI=10.1046/j.1365-2958.1998.00821.x;
RA   Moe P.C., Blount P., Kung C.;
RT   "Functional and structural conservation in the mechanosensitive channel
RT   mscL implicates elements crucial for mechanosensation.";
RL   Mol. Microbiol. 28:583-592(1998).
CC   -!- FUNCTION: Channel that opens in response to stretch forces in the
CC       membrane lipid bilayer. Forms a nonselective ion channel with a
CC       conductance of about 4 nanosiemens. May participate in the regulation
CC       of osmotic pressure changes within the cell.
CC       {ECO:0000269|PubMed:9632260}.
CC   -!- SUBUNIT: Homopentamer. {ECO:0000250|UniProtKB:P0A742,
CC       ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00115, ECO:0000269|PubMed:9632260}; Multi-pass membrane protein
CC       {ECO:0000250|UniProtKB:P0A742, ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SIMILARITY: Belongs to the MscL family. {ECO:0000255|HAMAP-
CC       Rule:MF_00115, ECO:0000305}.
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DR   EMBL; AF029732; AAC38561.1; -; Genomic_DNA.
DR   AlphaFoldDB; O68286; -.
DR   SMR; O68286; -.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0008381; F:mechanosensitive ion channel activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.1200.120; -; 1.
DR   HAMAP; MF_00115; MscL; 1.
DR   InterPro; IPR019823; Mechanosensitive_channel_CS.
DR   InterPro; IPR001185; MS_channel.
DR   InterPro; IPR037673; MSC/AndL.
DR   InterPro; IPR036019; MscL_channel.
DR   PANTHER; PTHR30266; PTHR30266; 1.
DR   Pfam; PF01741; MscL; 1.
DR   PRINTS; PR01264; MECHCHANNEL.
DR   SUPFAM; SSF81330; SSF81330; 1.
DR   TIGRFAMs; TIGR00220; mscL; 1.
DR   PROSITE; PS01327; MSCL; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Ion channel; Ion transport; Membrane;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..136
FT                   /note="Large-conductance mechanosensitive channel"
FT                   /id="PRO_0000192455"
FT   TOPO_DOM        1..16
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P9WJN5"
FT   TRANSMEM        17..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P9WJN5"
FT   TOPO_DOM        46..73
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250|UniProtKB:P9WJN5"
FT   TRANSMEM        74..93
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P9WJN5"
FT   TOPO_DOM        94..136
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P9WJN5"
SQ   SEQUENCE   136 AA;  14459 MW;  99DACA716E481095 CRC64;
     MGLLSEFKAF AVKGNVVDMA VGIIIGAAFG KIVSSFVGDV IMPPIGLLIG GVDFSDLAIT
     LKAEGDVPAV VLAYRKFIQT VLNFVIVAFA IFMGVKAINR LKREEAVAPS EPPVPSAEET
     LLTEIRDLLK AQQNKS