ID   MSCL_SALTY              Reviewed;         137 AA.
AC   P0A1X8; P39446; Q9KH32;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Large-conductance mechanosensitive channel {ECO:0000255|HAMAP-Rule:MF_00115};
GN   Name=mscL {ECO:0000255|HAMAP-Rule:MF_00115}; OrderedLocusNames=STM3410;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Jones S.E., Naik R.R., Stone M.O.;
RT   "Cloning and characterization of Salmonella typhimurium MscL.";
RL   Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-7.
RX   PubMed=8076592; DOI=10.1002/j.1460-2075.1994.tb06712.x;
RA   Parra-Lopez C., Lin R., Aspedon A., Groisman E.A.;
RT   "A Salmonella protein that is required for resistance to antimicrobial
RT   peptides and transport of potassium.";
RL   EMBO J. 13:3964-3972(1994).
CC   -!- FUNCTION: Channel that opens in response to stretch forces in the
CC       membrane lipid bilayer. May participate in the regulation of osmotic
CC       pressure changes within the cell. {ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SUBUNIT: Homopentamer. {ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00115}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_00115}.
CC   -!- SIMILARITY: Belongs to the MscL family. {ECO:0000255|HAMAP-
CC       Rule:MF_00115, ECO:0000305}.
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DR   EMBL; AF272843; AAF78240.1; -; Genomic_DNA.
DR   EMBL; AE006468; AAL22273.1; -; Genomic_DNA.
DR   EMBL; X80501; CAA56664.1; -; Genomic_DNA.
DR   RefSeq; NP_462314.1; NC_003197.2.
DR   RefSeq; WP_000008119.1; NC_003197.2.
DR   AlphaFoldDB; P0A1X8; -.
DR   SMR; P0A1X8; -.
DR   STRING; 99287.STM3410; -.
DR   PaxDb; P0A1X8; -.
DR   EnsemblBacteria; AAL22273; AAL22273; STM3410.
DR   GeneID; 1254933; -.
DR   KEGG; stm:STM3410; -.
DR   PATRIC; fig|99287.12.peg.3608; -.
DR   HOGENOM; CLU_095787_0_0_6; -.
DR   OMA; AWIIFLM; -.
DR   PhylomeDB; P0A1X8; -.
DR   BioCyc; SENT99287:STM3410-MON; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0008381; F:mechanosensitive ion channel activity; IBA:GO_Central.
DR   GO; GO:0009992; P:cellular water homeostasis; IBA:GO_Central.
DR   GO; GO:0006811; P:ion transport; IBA:GO_Central.
DR   Gene3D; 1.10.1200.120; -; 1.
DR   HAMAP; MF_00115; MscL; 1.
DR   InterPro; IPR019823; Mechanosensitive_channel_CS.
DR   InterPro; IPR001185; MS_channel.
DR   InterPro; IPR037673; MSC/AndL.
DR   InterPro; IPR036019; MscL_channel.
DR   PANTHER; PTHR30266; PTHR30266; 1.
DR   Pfam; PF01741; MscL; 1.
DR   PRINTS; PR01264; MECHCHANNEL.
DR   SUPFAM; SSF81330; SSF81330; 1.
DR   TIGRFAMs; TIGR00220; mscL; 1.
DR   PROSITE; PS01327; MSCL; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Ion channel; Ion transport; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..137
FT                   /note="Large-conductance mechanosensitive channel"
FT                   /id="PRO_0000192459"
FT   TRANSMEM        10..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00115"
FT   TRANSMEM        76..96
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00115"
FT   CONFLICT        59
FT                   /note="F -> V (in Ref. 1; AAF78240)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        63
FT                   /note="E -> D (in Ref. 1; AAF78240)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        86
FT                   /note="V -> L (in Ref. 1; AAF78240)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        94
FT                   /note="V -> M (in Ref. 1; AAF78240)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        101
FT                   /note="R -> K (in Ref. 1; AAF78240)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        107
FT                   /note="Missing (in Ref. 1; AAF78240)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        113
FT                   /note="P -> V (in Ref. 1; AAF78240)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        124
FT                   /note="G -> T (in Ref. 1; AAF78240)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        137
FT                   /note="S -> P (in Ref. 1; AAF78240)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   137 AA;  15074 MW;  BD9965994322B90A CRC64;
     MSFIKEFREF AMRGNVVDLA VGVIIGAAFG KIVSSLVADI IMPPLGLLIG GIDFKQFAFT
     LREAQGDIPA VVMHYGVFIQ NVFDFVIVAF AIFVAIKLIN RLNRKKAEEP AAPPAPSKEE
     VLLGEIRDLL KEQNNRS