ID   MSCL_SHEON              Reviewed;         136 AA.
AC   Q8EJE5;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Large-conductance mechanosensitive channel {ECO:0000255|HAMAP-Rule:MF_00115};
GN   Name=mscL {ECO:0000255|HAMAP-Rule:MF_00115}; OrderedLocusNames=SO_0522;
OS   Shewanella oneidensis (strain MR-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=211586;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MR-1;
RX   PubMed=12368813; DOI=10.1038/nbt749;
RA   Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C.,
RA   Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A.,
RA   Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C.,
RA   DeBoy R.T., Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R.,
RA   Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J.,
RA   Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J.,
RA   Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V.,
RA   Smith H.O., Venter J.C., Nealson K.H., Fraser C.M.;
RT   "Genome sequence of the dissimilatory metal ion-reducing bacterium
RT   Shewanella oneidensis.";
RL   Nat. Biotechnol. 20:1118-1123(2002).
CC   -!- FUNCTION: Channel that opens in response to stretch forces in the
CC       membrane lipid bilayer. May participate in the regulation of osmotic
CC       pressure changes within the cell. {ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SUBUNIT: Homopentamer. {ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00115}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_00115}.
CC   -!- SIMILARITY: Belongs to the MscL family. {ECO:0000255|HAMAP-
CC       Rule:MF_00115}.
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DR   EMBL; AE014299; AAN53603.1; -; Genomic_DNA.
DR   RefSeq; NP_716158.1; NC_004347.2.
DR   RefSeq; WP_011070864.1; NZ_CP053946.1.
DR   AlphaFoldDB; Q8EJE5; -.
DR   SMR; Q8EJE5; -.
DR   STRING; 211586.SO_0522; -.
DR   PaxDb; Q8EJE5; -.
DR   KEGG; son:SO_0522; -.
DR   PATRIC; fig|211586.12.peg.502; -.
DR   eggNOG; COG1970; Bacteria.
DR   HOGENOM; CLU_095787_0_0_6; -.
DR   OMA; AWIIFLM; -.
DR   OrthoDB; 1863650at2; -.
DR   PhylomeDB; Q8EJE5; -.
DR   BioCyc; SONE211586:G1GMP-494-MON; -.
DR   Proteomes; UP000008186; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0008381; F:mechanosensitive ion channel activity; IBA:GO_Central.
DR   GO; GO:0009992; P:cellular water homeostasis; IBA:GO_Central.
DR   GO; GO:0006811; P:ion transport; IBA:GO_Central.
DR   Gene3D; 1.10.1200.120; -; 1.
DR   HAMAP; MF_00115; MscL; 1.
DR   InterPro; IPR019823; Mechanosensitive_channel_CS.
DR   InterPro; IPR001185; MS_channel.
DR   InterPro; IPR037673; MSC/AndL.
DR   InterPro; IPR036019; MscL_channel.
DR   PANTHER; PTHR30266; PTHR30266; 1.
DR   Pfam; PF01741; MscL; 1.
DR   PRINTS; PR01264; MECHCHANNEL.
DR   SUPFAM; SSF81330; SSF81330; 1.
DR   TIGRFAMs; TIGR00220; mscL; 1.
DR   PROSITE; PS01327; MSCL; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Ion channel; Ion transport; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..136
FT                   /note="Large-conductance mechanosensitive channel"
FT                   /id="PRO_0000238033"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00115"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00115"
FT   TRANSMEM        54..74
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00115"
FT   TRANSMEM        79..99
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00115"
SQ   SEQUENCE   136 AA;  14475 MW;  82A7446C1EA4B35F CRC64;
     MSLIQEFKAF ASRGNVIDMA VGIIIGAAFG KIVSSFVADI IMPPIGIILG GVNFSDLSVV
     LLAAQGDAPA VVIAYGKFIQ TVIDFTIIAF AIFMGLKAIN SLKRKQEEAP PASPAPTKDQ
     ELLSEIRDLL KAQQEK