ID   MSCL_STAA3              Reviewed;         120 AA.
AC   Q2FH87;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 2.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Large-conductance mechanosensitive channel {ECO:0000255|HAMAP-Rule:MF_00115};
GN   Name=mscL {ECO:0000255|HAMAP-Rule:MF_00115};
GN   OrderedLocusNames=SAUSA300_1244;
OS   Staphylococcus aureus (strain USA300).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=367830;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=USA300;
RX   PubMed=16517273; DOI=10.1016/s0140-6736(06)68231-7;
RA   Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G.,
RA   Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F.,
RA   Perdreau-Remington F.;
RT   "Complete genome sequence of USA300, an epidemic clone of community-
RT   acquired meticillin-resistant Staphylococcus aureus.";
RL   Lancet 367:731-739(2006).
CC   -!- FUNCTION: Channel that opens in response to stretch forces in the
CC       membrane lipid bilayer. May participate in the regulation of osmotic
CC       pressure changes within the cell. {ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SUBUNIT: Homopentamer. {ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00115};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_00115}.
CC   -!- SIMILARITY: Belongs to the MscL family. {ECO:0000255|HAMAP-
CC       Rule:MF_00115}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABD21377.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000255; ABD21377.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_000910489.1; NZ_CP027476.1.
DR   AlphaFoldDB; Q2FH87; -.
DR   SMR; Q2FH87; -.
DR   EnsemblBacteria; ABD21377; ABD21377; SAUSA300_1244.
DR   KEGG; saa:SAUSA300_1244; -.
DR   HOGENOM; CLU_095787_0_0_9; -.
DR   Proteomes; UP000001939; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0008381; F:mechanosensitive ion channel activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.1200.120; -; 1.
DR   HAMAP; MF_00115; MscL; 1.
DR   InterPro; IPR019823; Mechanosensitive_channel_CS.
DR   InterPro; IPR001185; MS_channel.
DR   InterPro; IPR037673; MSC/AndL.
DR   InterPro; IPR036019; MscL_channel.
DR   PANTHER; PTHR30266; PTHR30266; 1.
DR   Pfam; PF01741; MscL; 1.
DR   PRINTS; PR01264; MECHCHANNEL.
DR   SUPFAM; SSF81330; SSF81330; 1.
DR   TIGRFAMs; TIGR00220; mscL; 1.
DR   PROSITE; PS01327; MSCL; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Ion channel; Ion transport; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..120
FT                   /note="Large-conductance mechanosensitive channel"
FT                   /id="PRO_0000238039"
FT   TRANSMEM        7..27
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00115"
FT   TRANSMEM        64..84
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00115"
SQ   SEQUENCE   120 AA;  13616 MW;  A4D1E6B2A7B7D2E5 CRC64;
     MLKEFKEFAL KGNVLDLAIA VVMGAAFNKI ISSLVENIIM PLIGKIFGSV DFAKEWSFWG
     IKYGLFIQSV IDFIIIAFAL FIFVKIANTL MKKEEAEEEA VVEENVVLLT EIRDLLREKK