ID   MSCS_EDWI9              Reviewed;         286 AA.
AC   O52401; C5BAU4;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   25-MAY-2022, entry version 122.
DE   RecName: Full=Small-conductance mechanosensitive channel;
GN   Name=mscS; OrderedLocusNames=NT01EI_3366;
OS   Edwardsiella ictaluri (strain 93-146).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Hafniaceae; Edwardsiella.
OX   NCBI_TaxID=634503;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=12542086; DOI=10.3354/dao052093;
RA   Moore M.M., Fernandez D.L., Thune R.L.;
RT   "Cloning and characterization of Edwardsiella ictaluri proteins expressed
RT   and recognized by the channel catfish Ictalurus punctatus immune response
RT   during infection.";
RL   Dis. Aquat. Organ. 52:93-107(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=93-146;
RA   Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C.,
RA   Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.;
RT   "Complete genome sequence of Edwardsiella ictaluri 93-146.";
RL   Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Mechanosensitive channel that participates in the regulation
CC       of osmotic pressure changes within the cell, opening in response to
CC       stretch forces in the membrane lipid bilayer, without the need for
CC       other proteins. Contributes to normal resistance to hypoosmotic shock.
CC       Forms an ion channel of 1.0 nanosiemens conductance with a slight
CC       preference for anions. The channel is sensitive to voltage; as the
CC       membrane is depolarized, less tension is required to open the channel
CC       and vice versa. The channel is characterized by short bursts of
CC       activity that last for a few seconds. {ECO:0000250|UniProtKB:P0C0S1}.
CC   -!- SUBUNIT: Homoheptamer. {ECO:0000250|UniProtKB:P0C0S1}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P0C0S1}; Multi-pass membrane protein
CC       {ECO:0000250|UniProtKB:P0C0S1}.
CC   -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family. {ECO:0000305}.
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DR   EMBL; AF037440; AAB92571.1; -; Genomic_DNA.
DR   EMBL; CP001600; ACR70503.1; -; Genomic_DNA.
DR   RefSeq; WP_015872576.1; NC_012779.2.
DR   AlphaFoldDB; O52401; -.
DR   SMR; O52401; -.
DR   EnsemblBacteria; ACR70503; ACR70503; NT01EI_3366.
DR   GeneID; 7959665; -.
DR   KEGG; eic:NT01EI_3366; -.
DR   PATRIC; fig|634503.3.peg.2992; -.
DR   HOGENOM; CLU_037945_1_1_6; -.
DR   OMA; GMQDTLT; -.
DR   OrthoDB; 1903165at2; -.
DR   Proteomes; UP000001485; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008381; F:mechanosensitive ion channel activity; IEA:InterPro.
DR   Gene3D; 2.30.30.60; -; 1.
DR   InterPro; IPR010920; LSM_dom_sf.
DR   InterPro; IPR045275; MscS_archaea/bacteria_type.
DR   InterPro; IPR006685; MscS_channel.
DR   InterPro; IPR011066; MscS_channel_C.
DR   InterPro; IPR006686; MscS_channel_CS.
DR   InterPro; IPR011014; MscS_channel_TM-2.
DR   InterPro; IPR023408; MscS_dom_sf.
DR   InterPro; IPR008910; TM_helix.
DR   PANTHER; PTHR30221; PTHR30221; 1.
DR   Pfam; PF00924; MS_channel; 1.
DR   Pfam; PF05552; TM_helix; 1.
DR   SUPFAM; SSF50182; SSF50182; 1.
DR   SUPFAM; SSF82689; SSF82689; 1.
DR   SUPFAM; SSF82861; SSF82861; 1.
DR   PROSITE; PS01246; UPF0003; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Ion channel; Ion transport; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..286
FT                   /note="Small-conductance mechanosensitive channel"
FT                   /id="PRO_0000110237"
FT   TOPO_DOM        1..30
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P0C0S1"
FT   TRANSMEM        31..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P0C0S1"
FT   TOPO_DOM        53..67
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P0C0S1"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P0C0S1"
FT   TOPO_DOM        89..90
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P0C0S1"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P0C0S1"
FT   TOPO_DOM        112..286
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P0C0S1"
SQ   SEQUENCE   286 AA;  30648 MW;  0B2442E07C70835D CRC64;
     MKELDVVDGI QSAGGWIVRN QDLLLGYAVN LVAAVVILII GSLIARGIST TLIRLLKARG
     LDVTVVHFLA AMVRYAILAF TIIAALGRLG VQTTSVIAVL GAAGLAVGLA LQGSLSNFAA
     GVLLVLFRPF RAGEVVDLGG VTGTVREVQI FSTTLATADN KVIVVPNGKI IAGNIINFSR
     EPKRRIDIIV GVAYDADIDV VKRVLGDVVA ADTRILHDDG VTIRLNEMAA SSLNFVVRVW
     GNNADYWAIY FDLMENFKRA LDANNIGIPF PQMDVHLYQA VKARAE