ID   MSCS_WIGBR              Reviewed;         280 AA.
AC   P0DKS1;
DT   28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2012, sequence version 1.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=Small-conductance mechanosensitive channel;
GN   Name=mscS; Synonyms=WGpWb0005; OrderedLocusNames=WIGBRp0050;
OS   Wigglesworthia glossinidia brevipalpis.
OG   Plasmid pWb1 (pWig1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Wigglesworthia.
OX   NCBI_TaxID=36870;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12219091; DOI=10.1038/ng986;
RA   Akman L., Yamashita A., Watanabe H., Oshima K., Shiba T., Hattori M.,
RA   Aksoy S.;
RT   "Genome sequence of the endocellular obligate symbiont of tsetse flies,
RT   Wigglesworthia glossinidia.";
RL   Nat. Genet. 32:402-407(2002).
CC   -!- FUNCTION: Mechanosensitive channel that participates in the regulation
CC       of osmotic pressure changes within the cell, opening in response to
CC       stretch forces in the membrane lipid bilayer, without the need for
CC       other proteins. Contributes to normal resistance to hypoosmotic shock.
CC       Forms an ion channel of 1.0 nanosiemens conductance with a slight
CC       preference for anions. {ECO:0000250|UniProtKB:P0C0S1}.
CC   -!- SUBUNIT: Homoheptamer. {ECO:0000250|UniProtKB:P0C0S1}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P0C0S1}; Multi-pass membrane protein
CC       {ECO:0000250|UniProtKB:P0C0S1}.
CC   -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB063523; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; WP_011013216.1; NC_003425.1.
DR   AlphaFoldDB; P0DKS1; -.
DR   SMR; P0DKS1; -.
DR   OrthoDB; 1903165at2; -.
DR   Proteomes; UP000000562; Plasmid pWb1.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008381; F:mechanosensitive ion channel activity; IEA:InterPro.
DR   Gene3D; 2.30.30.60; -; 1.
DR   InterPro; IPR010920; LSM_dom_sf.
DR   InterPro; IPR045275; MscS_archaea/bacteria_type.
DR   InterPro; IPR006685; MscS_channel.
DR   InterPro; IPR011066; MscS_channel_C.
DR   InterPro; IPR011014; MscS_channel_TM-2.
DR   InterPro; IPR023408; MscS_dom_sf.
DR   InterPro; IPR008910; TM_helix.
DR   PANTHER; PTHR30221; PTHR30221; 1.
DR   Pfam; PF00924; MS_channel; 1.
DR   Pfam; PF05552; TM_helix; 1.
DR   SUPFAM; SSF50182; SSF50182; 1.
DR   SUPFAM; SSF82689; SSF82689; 1.
DR   SUPFAM; SSF82861; SSF82861; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Ion channel; Ion transport; Membrane;
KW   Plasmid; Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..280
FT                   /note="Small-conductance mechanosensitive channel"
FT                   /id="PRO_0000420408"
FT   TOPO_DOM        1..27
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P0C0S1"
FT   TRANSMEM        28..49
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P0C0S1"
FT   TOPO_DOM        50..64
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P0C0S1"
FT   TRANSMEM        65..85
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P0C0S1"
FT   TOPO_DOM        86..87
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P0C0S1"
FT   TRANSMEM        88..108
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P0C0S1"
FT   TOPO_DOM        109..280
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P0C0S1"
SQ   SEQUENCE   280 AA;  31701 MW;  0440C904DA1882AD CRC64;
     MNIYKEIDNA SNWISDNHIL FIKYISNIIL SLIILIVGYS ASKVTQKIIK NFMIKKNIDI
     IISEFFCSII KYSILIFTIV TSLGCIGIQT TSIIAVIGAA GIAIGLALQG SLSNFAAGVL
     LVTLRYFRTG DYVNLCGVKG KIKTVQIFCT KIKTKDGKII IIPNNKIISS NIINYSEELH
     RLMEVIISTE YTSDIKNVKE IIIDVLKKET RIVKEKKITV RLKNLGESSL DFLVRGWVYK
     KELKQTTSDI LEKIKIELDK NKINIPYKQI DVNLKYSKEK