ID   MSD12_AMABI             Reviewed;          34 AA.
AC   A8W7P0;
DT   28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 1.
DT   25-MAY-2022, entry version 15.
DE   RecName: Full=MSDIN-like toxin proprotein 12 {ECO:0000303|PubMed:18025465};
DE   Contains:
DE     RecName: Full=Toxin MSD12 {ECO:0000303|PubMed:18025465};
DE   Flags: Precursor;
GN   Name=MSD12 {ECO:0000303|PubMed:18025465};
OS   Amanita bisporigera (Destroying angel).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Amanitaceae; Amanita.
OX   NCBI_TaxID=87325;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX   PubMed=18025465; DOI=10.1073/pnas.0707340104;
RA   Hallen H.E., Luo H., Scott-Craig J.S., Walton J.D.;
RT   "Gene family encoding the major toxins of lethal Amanita mushrooms.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:19097-19101(2007).
CC   -!- FUNCTION: Probable toxin that belongs to the MSDIN-like toxin family
CC       responsible for a large number of food poisoning cases and deaths
CC       (PubMed:18025465). {ECO:0000305|PubMed:18025465}.
CC   -!- PTM: Processed by the macrocyclase-peptidase enzyme POPB to yield a
CC       toxic cyclic nonapeptide (PubMed:18025465). POPB first removes 10
CC       residues from the N-terminus (By similarity). Conformational trapping
CC       of the remaining peptide forces the enzyme to release this intermediate
CC       rather than proceed to macrocyclization (By similarity). The enzyme
CC       rebinds the remaining peptide in a different conformation and catalyzes
CC       macrocyclization of the N-terminal 9 residues (By similarity).
CC       {ECO:0000250|UniProtKB:A0A067SLB9, ECO:0000305|PubMed:18025465}.
CC   -!- SIMILARITY: Belongs to the MSDIN fungal toxin family. {ECO:0000305}.
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DR   EMBL; EU196155; ABW87784.1; -; Genomic_DNA.
DR   AlphaFoldDB; A8W7P0; -.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR027582; Amanitin/phalloidin.
DR   TIGRFAMs; TIGR04309; amanitin; 1.
PE   3: Inferred from homology;
KW   Toxin.
FT   PROPEP          1..10
FT                   /evidence="ECO:0000305|PubMed:18025465"
FT                   /id="PRO_0000443668"
FT   PEPTIDE         11..19
FT                   /note="Toxin MSD12"
FT                   /evidence="ECO:0000305|PubMed:18025465"
FT                   /id="PRO_0000443669"
FT   PROPEP          20..34
FT                   /evidence="ECO:0000305|PubMed:18025465"
FT                   /id="PRO_0000443670"
FT   CROSSLNK        11..19
FT                   /note="Cyclopeptide (His-Pro)"
FT                   /evidence="ECO:0000305|PubMed:18025465"
SQ   SEQUENCE   34 AA;  3579 MW;  C38FD56BF17FF224 CRC64;
     MSDINATRLP HPFPLGLQPC AGDVDNLTLT KGEG