MSD1_DROME
ID MSD1_DROME Reviewed; 138 AA.
AC Q9W0G7; Q6NMY7;
DT 18-JAN-2017, integrated into UniProtKB/Swiss-Prot.
DT 21-FEB-2006, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Augmin complex subunit msd1 {ECO:0000305};
DE AltName: Full=Mitotic spindle density protein 1 {ECO:0000303|PubMed:19684111};
GN Name=msd1 {ECO:0000303|PubMed:19684111, ECO:0000312|FlyBase:FBgn0035209};
GN Synonyms=dgt9 {ECO:0000303|PubMed:19369198};
GN ORFNames=CG13914 {ECO:0000312|FlyBase:FBgn0035209};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAS15650.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAS15650.1};
RA Stapleton M., Carlson J., Chavez C., Frise E., George R., Pacleb J.,
RA Park S., Wan K., Yu C., Rubin G.M., Celniker S.;
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000312|EMBL:ANY27402.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:ANY27402.1};
RA Florea S., Webb J.S., Jaromczyk J., Schardl C.L.;
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP FUNCTION, IDENTIFICATION IN AUGMIN COMPLEX, AND DISRUPTION PHENOTYPE.
RX PubMed=19684111; DOI=10.1101/gad.532209;
RA Wainman A., Buster D.W., Duncan T., Metz J., Ma A., Sharp D.,
RA Wakefield J.G.;
RT "A new Augmin subunit, Msd1, demonstrates the importance of mitotic
RT spindle-templated microtubule nucleation in the absence of functioning
RT centrosomes.";
RL Genes Dev. 23:1876-1881(2009).
RN [6] {ECO:0000305}
RP IDENTIFICATION IN THE AUGMIN COMPLEX, SUBCELLULAR LOCATION, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=19369198; DOI=10.1073/pnas.0901587106;
RA Uehara R., Nozawa R.-S., Tomioka A., Petry S., Vale R.D., Obuse C.,
RA Goshima G.;
RT "The augmin complex plays a critical role in spindle microtubule generation
RT for mitotic progression and cytokinesis in human cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:6998-7003(2009).
CC -!- FUNCTION: As part of the augmin complex, plays a role in centrosome-
CC independent generation of spindle microtubules. The complex is required
CC for mitotic spindle assembly through its involvement in localizing
CC gamma-tubulin to spindle microtubules. msd1 is required for microtubule
CC nucleation from within the mitotic spindle and for localization of
CC Grip71 to centrosomes and mitotic spindle.
CC {ECO:0000269|PubMed:19684111}.
CC -!- SUBUNIT: Component of the augmin complex composed of dgt2, dgt3, dgt4,
CC dgt5, dgt6, msd1, msd5 and wac (PubMed:19684111, PubMed:19369198). The
CC complex interacts directly or indirectly with microtubules and is
CC required for centrosome-independent generation of spindle microtubules
CC (PubMed:19684111). {ECO:0000269|PubMed:19369198,
CC ECO:0000269|PubMed:19684111}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle
CC {ECO:0000269|PubMed:19369198}.
CC -!- DISRUPTION PHENOTYPE: Viable but females are sterile. Mutant embryos
CC show increased mitotic spindle length during metaphase and reduced
CC mitotic spindle density. {ECO:0000269|PubMed:19684111}.
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DR EMBL; AE014296; AAF47481.2; -; Genomic_DNA.
DR EMBL; BT011514; AAS15650.1; -; mRNA.
DR EMBL; KX531592; ANY27402.1; -; mRNA.
DR RefSeq; NP_612100.2; NM_138256.4.
DR AlphaFoldDB; Q9W0G7; -.
DR SMR; Q9W0G7; -.
DR DIP; DIP-48828N; -.
DR IntAct; Q9W0G7; 8.
DR STRING; 7227.FBpp0072571; -.
DR PaxDb; Q9W0G7; -.
DR DNASU; 38155; -.
DR EnsemblMetazoa; FBtr0072678; FBpp0072571; FBgn0035209.
DR GeneID; 38155; -.
DR KEGG; dme:Dmel_CG13914; -.
DR UCSC; CG13914-RA; d. melanogaster.
DR CTD; 38155; -.
DR FlyBase; FBgn0035209; msd1.
DR VEuPathDB; VectorBase:FBgn0035209; -.
DR eggNOG; ENOG502TC0W; Eukaryota.
DR HOGENOM; CLU_1857347_0_0_1; -.
DR InParanoid; Q9W0G7; -.
DR OMA; YRHRVEH; -.
DR OrthoDB; 1445311at2759; -.
DR PhylomeDB; Q9W0G7; -.
DR BioGRID-ORCS; 38155; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38155; -.
DR PRO; PR:Q9W0G7; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0035209; Expressed in secondary oocyte and 18 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007020; P:microtubule nucleation; IDA:FlyBase.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Cytoplasm; Cytoskeleton; Microtubule; Mitosis;
KW Reference proteome.
FT CHAIN 1..138
FT /note="Augmin complex subunit msd1"
FT /evidence="ECO:0000305"
FT /id="PRO_0000438656"
SQ SEQUENCE 138 AA; 15669 MW; 415EACFA2422829F CRC64;
MSDAVDKMLA GMAANRQTMN RQLAKIDEIM ERSNNTLLHI ESNSKAFSQN VALSETQKMY
NLRPEAEMTL SKILENFKLL MSSSDQREET YSALEGCLAY RHRVEHLGSS VRKLVALYDT
VGQMKNSQEE QYASEDSP
