MSD3_HUMAN
ID MSD3_HUMAN Reviewed; 275 AA.
AC Q96H12; B2RC35; Q5T726; Q5T727; Q5T728;
DT 26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Myb/SANT-like DNA-binding domain-containing protein 3;
GN Name=MSANTD3; Synonyms=C9orf30; ORFNames=L8;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RX PubMed=15334068; DOI=10.1038/sj.onc.1207921;
RA Petroziello J., Yamane A., Westendorf L., Thompson M., McDonagh C.,
RA Cerveny C., Law C.-L., Wahl A., Carter P.;
RT "Suppression subtractive hybridization and expression profiling identifies
RT a unique set of genes overexpressed in non-small-cell lung cancer.";
RL Oncogene 23:7734-7745(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Subthalamic nucleus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=17081983; DOI=10.1016/j.cell.2006.09.026;
RA Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.;
RT "Global, in vivo, and site-specific phosphorylation dynamics in signaling
RT networks.";
RL Cell 127:635-648(2006).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-98 AND SER-274, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-96; SER-98 AND SER-274, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT "System-wide temporal characterization of the proteome and phosphoproteome
RT of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-98, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [10]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-154, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=28112733; DOI=10.1038/nsmb.3366;
RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA Nielsen M.L.;
RT "Site-specific mapping of the human SUMO proteome reveals co-modification
RT with phosphorylation.";
RL Nat. Struct. Mol. Biol. 24:325-336(2017).
CC -!- INTERACTION:
CC Q96H12; P28329-3: CHAT; NbExp=3; IntAct=EBI-8466227, EBI-25837549;
CC Q96H12; P22607: FGFR3; NbExp=3; IntAct=EBI-8466227, EBI-348399;
CC Q96H12; Q14957: GRIN2C; NbExp=3; IntAct=EBI-8466227, EBI-8285963;
CC Q96H12; P06396: GSN; NbExp=3; IntAct=EBI-8466227, EBI-351506;
CC Q96H12; Q9Y649; NbExp=3; IntAct=EBI-8466227, EBI-25900580;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q96H12-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96H12-2; Sequence=VSP_053346, VSP_053347;
CC -!- TISSUE SPECIFICITY: Expressed in brain. {ECO:0000269|PubMed:15334068}.
CC -!- SIMILARITY: Belongs to the MSANTD3 family. {ECO:0000305}.
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DR EMBL; AY598327; AAT06738.1; -; mRNA.
DR EMBL; AK314924; BAG37432.1; -; mRNA.
DR EMBL; AL353805; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC008993; AAH08993.1; -; mRNA.
DR CCDS; CCDS56579.1; -. [Q96H12-2]
DR CCDS; CCDS6749.1; -. [Q96H12-1]
DR RefSeq; NP_001185734.1; NM_001198805.1. [Q96H12-1]
DR RefSeq; NP_001185735.1; NM_001198806.1. [Q96H12-1]
DR RefSeq; NP_001185736.1; NM_001198807.1. [Q96H12-2]
DR RefSeq; NP_542386.1; NM_080655.2. [Q96H12-1]
DR AlphaFoldDB; Q96H12; -.
DR SMR; Q96H12; -.
DR BioGRID; 124810; 21.
DR IntAct; Q96H12; 24.
DR MINT; Q96H12; -.
DR STRING; 9606.ENSP00000480445; -.
DR iPTMnet; Q96H12; -.
DR PhosphoSitePlus; Q96H12; -.
DR BioMuta; MSANTD3; -.
DR DMDM; 74751897; -.
DR EPD; Q96H12; -.
DR jPOST; Q96H12; -.
DR MassIVE; Q96H12; -.
DR MaxQB; Q96H12; -.
DR PaxDb; Q96H12; -.
DR PeptideAtlas; Q96H12; -.
DR PRIDE; Q96H12; -.
DR ProteomicsDB; 76693; -. [Q96H12-1]
DR Antibodypedia; 34901; 77 antibodies from 13 providers.
DR DNASU; 91283; -.
DR Ensembl; ENST00000374885.5; ENSP00000364020.1; ENSG00000066697.15. [Q96H12-2]
DR Ensembl; ENST00000395067.7; ENSP00000378506.2; ENSG00000066697.15. [Q96H12-1]
DR Ensembl; ENST00000613183.1; ENSP00000480445.1; ENSG00000066697.15. [Q96H12-1]
DR Ensembl; ENST00000622639.4; ENSP00000480622.1; ENSG00000066697.15. [Q96H12-1]
DR GeneID; 91283; -.
DR KEGG; hsa:91283; -.
DR MANE-Select; ENST00000395067.7; ENSP00000378506.2; NM_080655.3; NP_542386.1.
DR UCSC; uc004baw.4; human. [Q96H12-1]
DR CTD; 91283; -.
DR DisGeNET; 91283; -.
DR GeneCards; MSANTD3; -.
DR HGNC; HGNC:23370; MSANTD3.
DR HPA; ENSG00000066697; Low tissue specificity.
DR neXtProt; NX_Q96H12; -.
DR OpenTargets; ENSG00000066697; -.
DR PharmGKB; PA142672311; -.
DR VEuPathDB; HostDB:ENSG00000066697; -.
DR eggNOG; ENOG502RFDD; Eukaryota.
DR GeneTree; ENSGT00390000007901; -.
DR HOGENOM; CLU_065066_0_0_1; -.
DR InParanoid; Q96H12; -.
DR OMA; FQVCEGT; -.
DR PhylomeDB; Q96H12; -.
DR TreeFam; TF328595; -.
DR PathwayCommons; Q96H12; -.
DR SignaLink; Q96H12; -.
DR BioGRID-ORCS; 91283; 17 hits in 1076 CRISPR screens.
DR ChiTaRS; MSANTD3; human.
DR GenomeRNAi; 91283; -.
DR Pharos; Q96H12; Tdark.
DR PRO; PR:Q96H12; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; Q96H12; protein.
DR Bgee; ENSG00000066697; Expressed in cartilage tissue and 181 other tissues.
DR ExpressionAtlas; Q96H12; baseline and differential.
DR Genevisible; Q96H12; HS.
DR InterPro; IPR026709; Msantd3.
DR InterPro; IPR028002; Myb_DNA-bind_5.
DR PANTHER; PTHR21632; PTHR21632; 1.
DR Pfam; PF13873; Myb_DNA-bind_5; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Isopeptide bond; Phosphoprotein;
KW Reference proteome; Ubl conjugation.
FT CHAIN 1..275
FT /note="Myb/SANT-like DNA-binding domain-containing protein
FT 3"
FT /id="PRO_0000292590"
FT DOMAIN 13..78
FT /note="Myb-like"
FT COILED 211..247
FT /evidence="ECO:0000255"
FT MOD_RES 96
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:20068231"
FT MOD_RES 98
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163"
FT MOD_RES 274
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:20068231"
FT CROSSLNK 154
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT VAR_SEQ 140..159
FT /note="ESFAVSNRELCDDEKEFIHF -> VFFQERKRSLELSIILSGTW (in
FT isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_053346"
FT VAR_SEQ 160..275
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_053347"
SQ SEQUENCE 275 AA; 32363 MW; 1CB06C2AD26FD135 CRC64;
MQNNEIIKPA KYFSELEKSI LLALVEKYKY VLECKKSDAR TIALKQRTWQ ALAHEYNSQP
SVSLRDFKQL KKCWENIKAR TKKIMAHERR EKVKRSVSPL LSTHVLGKEK IASMLPEQLY
FLQSPPEEEP EYHPDASAQE SFAVSNRELC DDEKEFIHFP VCEGTSQPEP SCSAVRITAN
KNYRSKTSQE GALKKMHEEE HHQQMSILQL QLIQMNEVHV AKIQQIEREC EMAEEEHRIK
MEVLNKKKMY WERKLQTFTK EWPVSSFNRP FPNSP
