ID   MSD4_AMABI              Reviewed;          34 AA.
AC   A8W7N2;
DT   28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 1.
DT   25-MAY-2022, entry version 14.
DE   RecName: Full=MSDIN-like toxin proprotein 4 {ECO:0000303|PubMed:18025465};
DE   Contains:
DE     RecName: Full=Toxin MSD4 {ECO:0000303|PubMed:18025465};
DE   Flags: Precursor; Fragment;
GN   Name=MSD4 {ECO:0000303|PubMed:18025465};
OS   Amanita bisporigera (Destroying angel).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Amanitaceae; Amanita.
OX   NCBI_TaxID=87325;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX   PubMed=18025465; DOI=10.1073/pnas.0707340104;
RA   Hallen H.E., Luo H., Scott-Craig J.S., Walton J.D.;
RT   "Gene family encoding the major toxins of lethal Amanita mushrooms.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:19097-19101(2007).
CC   -!- FUNCTION: Probable toxin that belongs to the MSDIN-like toxin family
CC       responsible for a large number of food poisoning cases and deaths
CC       (PubMed:18025465). {ECO:0000305|PubMed:18025465}.
CC   -!- PTM: Processed by the macrocyclase-peptidase enzyme POPB to yield a
CC       toxic cyclic decapeptide (PubMed:18025465). POPB first removes 10
CC       residues from the N-terminus (By similarity). Conformational trapping
CC       of the remaining peptide forces the enzyme to release this intermediate
CC       rather than proceed to macrocyclization (By similarity). The enzyme
CC       rebinds the remaining peptide in a different conformation and catalyzes
CC       macrocyclization of the N-terminal 10 residues (By similarity).
CC       {ECO:0000250|UniProtKB:A0A067SLB9, ECO:0000305|PubMed:18025465}.
CC   -!- SIMILARITY: Belongs to the MSDIN fungal toxin family. {ECO:0000305}.
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DR   EMBL; EU196147; ABW87776.1; -; Genomic_DNA.
DR   AlphaFoldDB; A8W7N2; -.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR027582; Amanitin/phalloidin.
DR   TIGRFAMs; TIGR04309; amanitin; 1.
PE   3: Inferred from homology;
KW   Toxin.
FT   PROPEP          1..10
FT                   /evidence="ECO:0000305|PubMed:18025465"
FT                   /id="PRO_0000443644"
FT   PEPTIDE         11..20
FT                   /note="Toxin MSD4"
FT                   /evidence="ECO:0000305|PubMed:18025465"
FT                   /id="PRO_0000443645"
FT   PROPEP          21..34
FT                   /evidence="ECO:0000305|PubMed:18025465"
FT                   /id="PRO_0000443646"
FT   CROSSLNK        11..20
FT                   /note="Cyclopeptide (Leu-Pro)"
FT                   /evidence="ECO:0000305|PubMed:18025465"
FT   NON_TER         34
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   34 AA;  3784 MW;  9965541C99A4A98C CRC64;
     MSDINTARLP LFLPPVRMPP CVGDDIEMVL TRGE