ID   MSD4_AMAPH              Reviewed;          31 AA.
AC   A0A023IWE1;
DT   28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT   09-JUL-2014, sequence version 1.
DT   25-MAY-2022, entry version 10.
DE   RecName: Full=MSDIN-like toxin proprotein 4 {ECO:0000303|PubMed:24613547};
DE   Contains:
DE     RecName: Full=Toxin MSD4 {ECO:0000305|PubMed:24613547};
DE   Flags: Precursor;
OS   Amanita phalloides (Death cap).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Amanitaceae; Amanita.
OX   NCBI_TaxID=67723;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX   PubMed=24613547; DOI=10.1016/j.toxicon.2014.02.020;
RA   Li P., Deng W., Li T.;
RT   "The molecular diversity of toxin gene families in lethal Amanita
RT   mushrooms.";
RL   Toxicon 83:59-68(2014).
CC   -!- FUNCTION: Probable toxin that belongs to the MSDIN-like toxin family
CC       responsible for a large number of food poisoning cases and deaths
CC       (PubMed:24613547). {ECO:0000305|PubMed:24613547}.
CC   -!- PTM: Processed by the macrocyclase-peptidase enzyme POPB to yield a
CC       toxic cyclic hexapeptide (By similarity). POPB first removes 10
CC       residues from the N-terminus (By similarity). Conformational trapping
CC       of the remaining peptide forces the enzyme to release this intermediate
CC       rather than proceed to macrocyclization (By similarity). The enzyme
CC       rebinds the remaining peptide in a different conformation and catalyzes
CC       macrocyclization of the N-terminal 6 residues (By similarity).
CC       {ECO:0000250|UniProtKB:A0A067SLB9}.
CC   -!- SIMILARITY: Belongs to the MSDIN fungal toxin family. {ECO:0000305}.
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DR   EMBL; KF552085; AHB18713.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A023IWE1; -.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR027582; Amanitin/phalloidin.
DR   TIGRFAMs; TIGR04309; amanitin; 1.
PE   3: Inferred from homology;
KW   Toxin.
FT   PROPEP          1..10
FT                   /evidence="ECO:0000305|PubMed:24613547"
FT                   /id="PRO_0000443680"
FT   PEPTIDE         11..16
FT                   /note="Toxin MSD4"
FT                   /evidence="ECO:0000305|PubMed:24613547"
FT                   /id="PRO_0000443681"
FT   PROPEP          17..31
FT                   /evidence="ECO:0000305|PubMed:24613547"
FT                   /id="PRO_0000443682"
FT   CROSSLNK        11..16
FT                   /note="Cyclopeptide (Trp-Pro)"
FT                   /evidence="ECO:0000305|PubMed:24613547"
SQ   SEQUENCE   31 AA;  3389 MW;  16E609435DE5D1C8 CRC64;
     MSDINGTRLP WLATCPCVGE DVNPTLSRGE R