MSD5_DROME
ID MSD5_DROME Reviewed; 253 AA.
AC Q9W0G6;
DT 18-JAN-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Augmin complex subunit msd5 {ECO:0000305};
DE AltName: Full=Mitotic spindle density protein 5 {ECO:0000312|FlyBase:FBgn0035210};
GN Name=msd5 {ECO:0000312|FlyBase:FBgn0035210};
GN Synonyms=dgt7 {ECO:0000303|PubMed:19369198};
GN ORFNames=CG2213 {ECO:0000312|EMBL:AAL68303.1,
GN ECO:0000312|FlyBase:FBgn0035210};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAL68303.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL68303.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAL68303.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP IDENTIFICATION IN THE AUGMIN COMPLEX, SUBCELLULAR LOCATION, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=19369198; DOI=10.1073/pnas.0901587106;
RA Uehara R., Nozawa R.-S., Tomioka A., Petry S., Vale R.D., Obuse C.,
RA Goshima G.;
RT "The augmin complex plays a critical role in spindle microtubule generation
RT for mitotic progression and cytokinesis in human cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:6998-7003(2009).
CC -!- FUNCTION: As part of the augmin complex, plays a role in centrosome-
CC independent generation of spindle microtubules. The complex is required
CC for mitotic spindle assembly through its involvement in localizing
CC gamma-tubulin to spindle microtubules. {ECO:0000305|PubMed:19369198}.
CC -!- SUBUNIT: Component of the augmin complex composed of dgt2, dgt3, dgt4,
CC dgt5, dgt6, msd1, msd5 and wac (PubMed:19369198). The complex interacts
CC directly or indirectly with microtubules and is required for
CC centrosome-independent generation of spindle microtubules.
CC {ECO:0000269|PubMed:19369198}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle
CC {ECO:0000269|PubMed:19369198}.
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DR EMBL; AE014296; AAF47482.1; -; Genomic_DNA.
DR EMBL; AY075493; AAL68303.1; -; mRNA.
DR RefSeq; NP_612101.1; NM_138257.3.
DR AlphaFoldDB; Q9W0G6; -.
DR SMR; Q9W0G6; -.
DR IntAct; Q9W0G6; 4.
DR STRING; 7227.FBpp0072608; -.
DR PaxDb; Q9W0G6; -.
DR PRIDE; Q9W0G6; -.
DR DNASU; 38156; -.
DR EnsemblMetazoa; FBtr0072724; FBpp0072608; FBgn0035210.
DR GeneID; 38156; -.
DR KEGG; dme:Dmel_CG2213; -.
DR UCSC; CG2213-RA; d. melanogaster.
DR CTD; 38156; -.
DR FlyBase; FBgn0035210; msd5.
DR VEuPathDB; VectorBase:FBgn0035210; -.
DR eggNOG; ENOG502TBSC; Eukaryota.
DR HOGENOM; CLU_1162218_0_0_1; -.
DR InParanoid; Q9W0G6; -.
DR OMA; DEYPANL; -.
DR OrthoDB; 1149441at2759; -.
DR PhylomeDB; Q9W0G6; -.
DR BioGRID-ORCS; 38156; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38156; -.
DR PRO; PR:Q9W0G6; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0035210; Expressed in eye disc (Drosophila) and 20 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Cytoplasm; Cytoskeleton; Microtubule; Mitosis;
KW Reference proteome.
FT CHAIN 1..253
FT /note="Augmin complex subunit msd5"
FT /evidence="ECO:0000305"
FT /id="PRO_0000438657"
SQ SEQUENCE 253 AA; 28461 MW; 6F1CC76D68802740 CRC64;
METNVDFSSI SSKLYRKYAQ HVRNLKDVCV SKTVMKPGAF FDSLQQMMEE EAAATTPPRD
LSSVADYAEL FKTLEEYPAN LQKMPKKREL QRTNSTLLRG ADESVAMGIN TSNVSLSLTR
LEEQRSAVDV YNDFKGFQRK LAKIYDEAAA LDTTESIYKQ KLTQLHGFAQ QLEKLMPTGG
ESPPDAFTSE QQEKLLTIAA NMEQLNYLRS NSLQLPNPNE ILATGSLAAR LEMFVEVLTY
TLLQVSSYNM AMI
