MSGN1_MOUSE
ID MSGN1_MOUSE Reviewed; 188 AA.
AC Q9JK54;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Mesogenin-1;
DE AltName: Full=Paraxial mesoderm-specific mesogenin1;
DE AltName: Full=pMesogenin1;
DE Short=pMsgn1;
GN Name=Msgn1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=10837126; DOI=10.1006/dbio.2000.9717;
RA Yoon J.K., Moon R.T., Wold B.;
RT "The bHLH class protein pMesogenin1 can specify paraxial mesoderm
RT phenotypes.";
RL Dev. Biol. 222:376-391(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=11124811; DOI=10.1101/gad.850000;
RA Yoon J.K., Wold B.;
RT "The bHLH regulator pMesogenin1 is required for maturation and segmentation
RT of paraxial mesoderm.";
RL Genes Dev. 14:3204-3214(2000).
CC -!- FUNCTION: Involved in specifying the paraxial, but not dorsal,
CC mesoderm. May regulate the expression of T-box transcription factors
CC required for mesoderm formation and differentiation.
CC {ECO:0000269|PubMed:10837126, ECO:0000269|PubMed:11124811}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- DEVELOPMENTAL STAGE: Specifically expressed in unsegmented paraxial
CC mesoderm and its immediate progenitors and sharply down-regulated in
CC presumptive somites. Not detectable at 6.5 dpc. At 7.5 dpc, expressed
CC mainly in the posterior region of the embryo, lateral to the primitive
CC streak where presumptive progenitors of paraxial mesoderm reside.
CC Expression is largely excluded from the midline of the primitive
CC streak. No expression is detected in the anterior part of the embryo.
CC At 9.0 dpc, highly expressed in the caudal presomitic mesoderm. At 11.5
CC dpc, the primitive streak completely regresses, expression is observed
CC exclusively in the tailbud. Expression remains in the tailbud until
CC 13.5 dpc and begins to disappear between 13.5 and 14.5 dpc when the
CC tailbud loses its potential to provide paraxial mesoderm cells.
CC {ECO:0000269|PubMed:10837126}.
CC -!- DISRUPTION PHENOTYPE: Mutant animals die during gestation. Premature
CC death starts around 10.5 dpc. Severe morphological abnormalities, such
CC as enlarged tailbud, kinked neural tube posterior to forelimb buds and
CC reduced tissue mass in the interlimb domain become obvious at 9.0 dpc
CC and thereafter. Head and anterior body structures, including the
CC developing heart, appeared normal. In the trunk posterior to the
CC forelimbs, there are no identifiable somites or segment patterning,
CC although the anterior-most somites appeared normal, with decreases in
CC somite size and disruption of myotomal patterning in somites 8-11.
CC There are no detectable somites beyond somite 11. At 11.5 dpc, myotomes
CC are absent from the trunk at the interlimb level and at 12.5 dpc,
CC muscle is absent from the hindlimbs. The few fetuses surviving beyond
CC 14.5 and 17.5 pdc lack a tail and show a grossly normal, but very thin
CC body with relatively normal forelimbs and hindlimbs. At this stage, all
CC vertebrae posterior to cervical level together with ribs are absent,
CC while all cervical vertebrae are present, although the most posterior
CC ones are sometimes malformed and fused. Other trunk skeletal structures
CC that originate from lateral mesoderm, such as the sternum and scapula,
CC form quite normally. {ECO:0000269|PubMed:11124811}.
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DR EMBL; AF261105; AAF70375.1; -; mRNA.
DR EMBL; BC116996; AAI16997.1; -; mRNA.
DR CCDS; CCDS25814.1; -.
DR RefSeq; NP_062417.1; NM_019544.1.
DR AlphaFoldDB; Q9JK54; -.
DR SMR; Q9JK54; -.
DR STRING; 10090.ENSMUSP00000055001; -.
DR iPTMnet; Q9JK54; -.
DR PhosphoSitePlus; Q9JK54; -.
DR PaxDb; Q9JK54; -.
DR PRIDE; Q9JK54; -.
DR Antibodypedia; 64308; 71 antibodies from 13 providers.
DR DNASU; 56184; -.
DR Ensembl; ENSMUST00000049877; ENSMUSP00000055001; ENSMUSG00000047002.
DR GeneID; 56184; -.
DR KEGG; mmu:56184; -.
DR UCSC; uc007nau.1; mouse.
DR CTD; 343930; -.
DR MGI; MGI:1860483; Msgn1.
DR VEuPathDB; HostDB:ENSMUSG00000047002; -.
DR eggNOG; KOG4029; Eukaryota.
DR GeneTree; ENSGT00530000063712; -.
DR HOGENOM; CLU_084234_1_0_1; -.
DR InParanoid; Q9JK54; -.
DR OMA; SSWDWKN; -.
DR OrthoDB; 1493381at2759; -.
DR PhylomeDB; Q9JK54; -.
DR TreeFam; TF325707; -.
DR BioGRID-ORCS; 56184; 4 hits in 70 CRISPR screens.
DR PRO; PR:Q9JK54; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q9JK54; protein.
DR Bgee; ENSMUSG00000047002; Expressed in embryonic post-anal tail and 8 other tissues.
DR Genevisible; Q9JK54; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; IDA:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0001707; P:mesoderm formation; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007379; P:segment specification; IMP:MGI.
DR GO; GO:0001756; P:somitogenesis; IMP:MGI.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR040259; Mesogenin/MesP.
DR PANTHER; PTHR20937; PTHR20937; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..188
FT /note="Mesogenin-1"
FT /id="PRO_0000330029"
FT DOMAIN 118..172
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
SQ SEQUENCE 188 AA; 20553 MW; B31A67067630E322 CRC64;
MDNLGETFLS LEDGLDSSDT AGLLASWDWK SRARPLELVQ ESPTQSLSPA PSLESYSEVA
LPCGHSGAST GGSDGYGSHE AAGLVELDYS MLAFQPPYLH TAGGLKGQKG SKVKMSVQRR
RKASEREKLR MRTLADALHT LRNYLPPVYS QRGQPLTKIQ TLKYTIKYIG ELTDLLNSSG
REPRPQSV
