MSH1_ARATH
ID MSH1_ARATH Reviewed; 1118 AA.
AC Q84LK0; Q9LK12;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=DNA mismatch repair protein MSH1, mitochondrial;
DE Short=AtMSH1;
DE AltName: Full=MutS protein homolog 1;
DE AltName: Full=Protein CHLOROPLAST MUTATOR;
DE Flags: Precursor;
GN Name=MSH1; Synonyms=CHM, CHM1; OrderedLocusNames=At3g24320;
GN ORFNames=K7M2.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DISRUPTION
RP PHENOTYPE, AND MUTAGENESIS OF CYS-874.
RC STRAIN=cv. Columbia;
RX PubMed=12730382; DOI=10.1073/pnas.1037651100;
RA Abdelnoor R.V., Yule R., Elo A., Christensen A.C., Meyer-Gauen G.,
RA Mackenzie S.A.;
RT "Substoichiometric shifting in the plant mitochondrial genome is influenced
RT by a gene homologous to MutS.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:5968-5973(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=1356535; DOI=10.2307/3869457;
RA Martinez-Zapater J.M., Gil P., Capel J., Somerville C.R.;
RT "Mutations at the Arabidopsis CHM locus promote rearrangements of the
RT mitochondrial genome.";
RL Plant Cell 4:889-899(1992).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=16169894; DOI=10.1105/tpc.105.035287;
RA Christensen A.C., Lyznik A., Mohammed S., Elowsky C.G., Elo A., Yule R.,
RA Mackenzie S.A.;
RT "Dual-domain, dual-targeting organellar protein presequences in Arabidopsis
RT can use non-AUG start codons.";
RL Plant Cell 17:2805-2816(2005).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF ASP-847; CYS-874 AND
RP PRO-1018.
RX PubMed=16830098; DOI=10.1007/s00239-005-0226-9;
RA Abdelnoor R.V., Christensen A.C., Mohammed S., Munoz-Castillo B.,
RA Moriyama H., Mackenzie S.A.;
RT "Mitochondrial genome dynamics in plants and animals: convergent gene
RT fusions of a MutS homologue.";
RL J. Mol. Evol. 63:165-173(2006).
RN [7]
RP FUNCTION.
RX PubMed=19822729; DOI=10.1534/genetics.109.108514;
RA Arrieta-Montiel M.P., Shedge V., Davila J., Christensen A.C.,
RA Mackenzie S.A.;
RT "Diversity of the Arabidopsis mitochondrial genome occurs via nuclear-
RT controlled recombination activity.";
RL Genetics 183:1261-1268(2009).
RN [8]
RP FUNCTION.
RX PubMed=20139171; DOI=10.1104/pp.109.152827;
RA Shedge V., Davila J., Arrieta-Montiel M.P., Mohammed S., Mackenzie S.A.;
RT "Extensive rearrangement of the Arabidopsis mitochondrial genome elicits
RT cellular conditions for thermotolerance.";
RL Plant Physiol. 152:1960-1970(2010).
RN [9]
RP FUNCTION.
RX PubMed=22426777; DOI=10.1007/s00122-012-1844-4;
RA Sun P., Arrieta-Montiel M.P., Mackenzie S.A.;
RT "Utility of in vitro culture to the study of plant mitochondrial genome
RT configuration and its dynamic features.";
RL Theor. Appl. Genet. 125:449-454(2012).
CC -!- FUNCTION: DNA mismatch repair protein specifically involved in
CC maintenance of mitochondrial genome configuration by controlling
CC specific rearranged portion. Functions by suppressing asymmetric
CC recombination at some repeat pairs. {ECO:0000269|PubMed:12730382,
CC ECO:0000269|PubMed:16830098, ECO:0000269|PubMed:19822729,
CC ECO:0000269|PubMed:20139171, ECO:0000269|PubMed:22426777}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:12730382,
CC ECO:0000269|PubMed:16169894, ECO:0000269|PubMed:16830098}. Plastid,
CC chloroplast {ECO:0000269|PubMed:12730382, ECO:0000269|PubMed:16169894}.
CC Note=Predominantly targeted to the mitochondrion.
CC {ECO:0000269|PubMed:16169894}.
CC -!- DISRUPTION PHENOTYPE: Variegated plants and appearance of specific new
CC restriction fragments in the mitochondrial genome.
CC {ECO:0000269|PubMed:12730382, ECO:0000269|PubMed:1356535}.
CC -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB02932.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY191303; AAO49798.1; -; mRNA.
DR EMBL; AP000382; BAB02932.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE76889.1; -; Genomic_DNA.
DR RefSeq; NP_189075.2; NM_113339.4.
DR AlphaFoldDB; Q84LK0; -.
DR SMR; Q84LK0; -.
DR STRING; 3702.AT3G24320.1; -.
DR PaxDb; Q84LK0; -.
DR PRIDE; Q84LK0; -.
DR ProteomicsDB; 238913; -.
DR EnsemblPlants; AT3G24320.1; AT3G24320.1; AT3G24320.
DR GeneID; 822021; -.
DR Gramene; AT3G24320.1; AT3G24320.1; AT3G24320.
DR KEGG; ath:AT3G24320; -.
DR Araport; AT3G24320; -.
DR TAIR; locus:2087193; AT3G24320.
DR eggNOG; KOG0217; Eukaryota.
DR HOGENOM; CLU_002472_6_0_1; -.
DR InParanoid; Q84LK0; -.
DR OMA; LMGSITC; -.
DR OrthoDB; 138168at2759; -.
DR PhylomeDB; Q84LK0; -.
DR PRO; PR:Q84LK0; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q84LK0; baseline and differential.
DR Genevisible; Q84LK0; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0009536; C:plastid; IDA:TAIR.
DR GO; GO:0042651; C:thylakoid membrane; IDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR GO; GO:0032042; P:mitochondrial DNA metabolic process; IMP:TAIR.
DR GO; GO:0043504; P:mitochondrial DNA repair; IBA:GO_Central.
DR GO; GO:0000002; P:mitochondrial genome maintenance; IMP:TAIR.
DR GO; GO:0009408; P:response to heat; IGI:TAIR.
DR Gene3D; 3.40.1170.10; -; 1.
DR Gene3D; 3.40.1440.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR007695; DNA_mismatch_repair_MutS-lik_N.
DR InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR InterPro; IPR016151; DNA_mismatch_repair_MutS_N.
DR InterPro; IPR035901; GIY-YIG_endonuc_sf.
DR InterPro; IPR045076; MutS_family.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11361; PTHR11361; 1.
DR Pfam; PF01624; MutS_I; 1.
DR Pfam; PF00488; MutS_V; 1.
DR SMART; SM00534; MUTSac; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF55271; SSF55271; 1.
DR SUPFAM; SSF82771; SSF82771; 1.
DR PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Chloroplast; DNA damage; DNA repair; DNA-binding;
KW Mitochondrion; Nucleotide-binding; Plastid; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..?
FT /note="Chloroplast and mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN ?..1118
FT /note="DNA mismatch repair protein MSH1, mitochondrial"
FT /id="PRO_0000418366"
FT BINDING 768..775
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT MUTAGEN 847
FT /note="D->N: In chm1-5; variegated plant phenotype."
FT /evidence="ECO:0000269|PubMed:16830098"
FT MUTAGEN 874
FT /note="C->Y: In chm1-3; variegated plant phenotype."
FT /evidence="ECO:0000269|PubMed:12730382,
FT ECO:0000269|PubMed:16830098"
FT MUTAGEN 1018
FT /note="P->L: Variegated plant phenotype."
FT /evidence="ECO:0000269|PubMed:16830098"
SQ SEQUENCE 1118 AA; 123920 MW; 42084347C6A123CD CRC64;
MHWIATRNAV VSFPKWRFFF RSSYRTYSSL KPSSPILLNR RYSEGISCLR DGKSLKRITT
ASKKVKTSSD VLTDKDLSHL VWWKERLQTC KKPSTLQLIE RLMYTNLLGL DPSLRNGSLK
DGNLNWEMLQ FKSRFPREVL LCRVGEFYEA IGIDACILVE YAGLNPFGGL RSDSIPKAGC
PIMNLRQTLD DLTRNGYSVC IVEEVQGPTP ARSRKGRFIS GHAHPGSPYV YGLVGVDHDL
DFPDPMPVVG ISRSARGYCM ISIFETMKAY SLDDGLTEEA LVTKLRTRRC HHLFLHASLR
HNASGTCRWG EFGEGGLLWG ECSSRNFEWF EGDTLSELLS RVKDVYGLDD EVSFRNVNVP
SKNRPRPLHL GTATQIGALP TEGIPCLLKV LLPSTCSGLP SLYVRDLLLN PPAYDIALKI
QETCKLMSTV TCSIPEFTCV SSAKLVKLLE QREANYIEFC RIKNVLDDVL HMHRHAELVE
ILKLLMDPTW VATGLKIDFD TFVNECHWAS DTIGEMISLD ENESHQNVSK CDNVPNEFFY
DMESSWRGRV KGIHIEEEIT QVEKSAEALS LAVAEDFHPI ISRIKATTAS LGGPKGEIAY
AREHESVWFK GKRFTPSIWA GTAGEDQIKQ LKPALDSKGK KVGEEWFTTP KVEIALVRYH
EASENAKARV LELLRELSVK LQTKINVLVF ASMLLVISKA LFSHACEGRR RKWVFPTLVG
FSLDEGAKPL DGASRMKLTG LSPYWFDVSS GTAVHNTVDM QSLFLLTGPN GGGKSSLLRS
ICAAALLGIS GLMVPAESAC IPHFDSIMLH MKSYDSPVDG KSSFQVEMSE IRSIVSQATS
RSLVLIDEIC RGTETAKGTC IAGSVVESLD TSGCLGIVST HLHGIFSLPL TAKNITYKAM
GAENVEGQTK PTWKLTDGVC RESLAFETAK REGVPESVIQ RAEALYLSVY AKDASAEVVK
PDQIITSSNN DQQIQKPVSS ERSLEKDLAK AIVKICGKKM IEPEAIECLS IGARELPPPS
TVGSSCVYVM RRPDKRLYIG QTDDLEGRIR AHRAKEGLQG SSFLYLMVQG KSMACQLETL
LINQLHEQGY SLANLADGKH RNFGTSSSLS TSDVVSIL
