MSH2_DROME
ID MSH2_DROME Reviewed; 917 AA.
AC P43248; Q86BK6; Q9NKC4; Q9VJT0;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 4.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=DNA mismatch repair protein spellchecker 1;
GN Name=spel1; ORFNames=CG4215;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=Canton-S; TISSUE=Head;
RX PubMed=10077620; DOI=10.1073/pnas.96.6.2964;
RA Flores C., Engels W.;
RT "Microsatellite instability in Drosophila spellchecker1 (MutS homolog)
RT mutants.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:2964-2969(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10471707; DOI=10.1093/genetics/153.1.179;
RA Ashburner M., Misra S., Roote J., Lewis S.E., Blazej R.G., Davis T.,
RA Doyle C., Galle R.F., George R.A., Harris N.L., Hartzell G., Harvey D.A.,
RA Hong L., Houston K.A., Hoskins R.A., Johnson G., Martin C., Moshrefi A.R.,
RA Palazzolo M., Reese M.G., Spradling A.C., Tsang G., Wan K.H., Whitelaw K.,
RA Celniker S.E., Rubin G.M.;
RT "An exploration of the sequence of a 2.9-Mb region of the genome of
RT Drosophila melanogaster: the Adh region.";
RL Genetics 153:179-219(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- FUNCTION: Involved in postreplication mismatch repair. Binds
CC specifically to DNA containing mismatched nucleotides thus providing a
CC target for the excision repair processes characteristic of
CC postreplication mismatch repair (By similarity). {ECO:0000250,
CC ECO:0000269|PubMed:10077620}.
CC -!- SUBUNIT: Heterodimer of Msh2/Spel and Msh6. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA62406.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U17893; AAA62406.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AE014134; AAF53392.2; -; Genomic_DNA.
DR RefSeq; NP_001014482.2; NM_001014482.3.
DR RefSeq; NP_001246031.1; NM_001259102.2.
DR RefSeq; NP_001285931.1; NM_001299002.1.
DR RefSeq; NP_523565.2; NM_078841.5.
DR AlphaFoldDB; P43248; -.
DR SMR; P43248; -.
DR BioGRID; 60866; 6.
DR DIP; DIP-21499N; -.
DR IntAct; P43248; 2.
DR STRING; 7227.FBpp0301208; -.
DR PaxDb; P43248; -.
DR PRIDE; P43248; -.
DR EnsemblMetazoa; FBtr0080630; FBpp0080203; FBgn0015546.
DR EnsemblMetazoa; FBtr0309281; FBpp0301208; FBgn0015546.
DR EnsemblMetazoa; FBtr0344018; FBpp0310469; FBgn0015546.
DR EnsemblMetazoa; FBtr0344019; FBpp0310470; FBgn0015546.
DR GeneID; 34842; -.
DR KEGG; dme:Dmel_CG4215; -.
DR UCSC; CG4215-RA; d. melanogaster.
DR CTD; 34842; -.
DR FlyBase; FBgn0015546; spel1.
DR VEuPathDB; VectorBase:FBgn0015546; -.
DR eggNOG; KOG0219; Eukaryota.
DR GeneTree; ENSGT00550000074867; -.
DR HOGENOM; CLU_002472_10_0_1; -.
DR InParanoid; P43248; -.
DR OMA; LFRIYQV; -.
DR OrthoDB; 138168at2759; -.
DR PhylomeDB; P43248; -.
DR Reactome; R-DME-5358565; Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
DR BioGRID-ORCS; 34842; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 34842; -.
DR PRO; PR:P43248; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0015546; Expressed in cleaving embryo and 22 other tissues.
DR ExpressionAtlas; P43248; baseline and differential.
DR Genevisible; P43248; DM.
DR GO; GO:0032301; C:MutSalpha complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; NAS:UniProtKB.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0030983; F:mismatched DNA binding; IBA:GO_Central.
DR GO; GO:0036297; P:interstrand cross-link repair; IMP:FlyBase.
DR GO; GO:0043570; P:maintenance of DNA repeat elements; IMP:FlyBase.
DR GO; GO:0006298; P:mismatch repair; IMP:UniProtKB.
DR GO; GO:0006312; P:mitotic recombination; IBA:GO_Central.
DR GO; GO:0006301; P:postreplication repair; ISS:UniProtKB.
DR Gene3D; 3.30.420.110; -; 1.
DR Gene3D; 3.40.1170.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR011184; DNA_mismatch_repair_Msh2.
DR InterPro; IPR007695; DNA_mismatch_repair_MutS-lik_N.
DR InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR InterPro; IPR007861; DNA_mismatch_repair_MutS_clamp.
DR InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR InterPro; IPR016151; DNA_mismatch_repair_MutS_N.
DR InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR InterPro; IPR007860; DNA_mmatch_repair_MutS_con_dom.
DR InterPro; IPR032642; Msh2.
DR InterPro; IPR036678; MutS_con_dom_sf.
DR InterPro; IPR045076; MutS_family.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11361; PTHR11361; 1.
DR PANTHER; PTHR11361:SF35; PTHR11361:SF35; 1.
DR Pfam; PF01624; MutS_I; 1.
DR Pfam; PF05188; MutS_II; 1.
DR Pfam; PF05192; MutS_III; 1.
DR Pfam; PF05190; MutS_IV; 1.
DR Pfam; PF00488; MutS_V; 1.
DR PIRSF; PIRSF005813; MSH2; 1.
DR SMART; SM00534; MUTSac; 1.
DR SMART; SM00533; MUTSd; 1.
DR SUPFAM; SSF48334; SSF48334; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Nucleotide-binding;
KW Nucleus; Reference proteome.
FT CHAIN 1..917
FT /note="DNA mismatch repair protein spellchecker 1"
FT /id="PRO_0000115186"
FT BINDING 667..674
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 817
FT /note="S -> P (in Ref. 1; AAA62406)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 917 AA; 103313 MW; 42E1EF87A390ED27 CRC64;
MQAKATDSRQ EPTLNMDTNA RRNFIKFHAK LGEKPATTVR FFDHTDRYTV HGSDDCELVA
KIVYKSTAFI GALLPDDKKE TLQFVSMSKG NFELAVRELL LVRNYRVEVY VKNSSDWEIE
YRGSPGNLLQ FEDILFSNKE VLVGNSIISL LVKLDGGGQR RVGVASVEQN DCKFQLLEFL
DDDFFTELEA TVVLLGPKEC LLPSIEGEYS AVKTLLDRNG VMITMPKKSG DNDLLQDLNR
LLRFAKGQQE DATGLKELQL QLASNALKTA IKYLDLVNDA GNLGHYEIKQ LDLNRFVHLD
SAAVAALNIM PKPGTHPSMP SYRWQSVLGV LDHCRTPQGH RLMGQWVKQP LRSRNILNDR
HNIVQCLLES PDTMETLSLD YLKRIPDILM LTKKLMRRKA NLQDLFRIYQ VILRTPKILK
VLHELDNSTI ESVICAPFKS FLKDLTGLKQ MVEQVVDFEA IERGEYLVKA SFDSRLMELQ
QMMTELYSKM EELQFKCSQE LNLDGKNQVK LESVAKLGHH FRITVKDDSV LRKNKNYRIV
DVIKGGVRFT SDKLEGYADE FASCRTRYEE QQLSIVEEII HVAVGYAAPL TLLNNELAQL
DCLVSFAIAA RSAPTPYVRP KMLEEGAREL VLEDVRHPCL ELQEHVNFIA NSVDFKKEEC
NMFIITGPNM GGKSTYIRSV GTAVLMAHIG AFVPCSLATI SMVDSILGRV GASDNIIKGL
STFMVEMIET SGIIRTATDK SLVIIDELGR GTSTYEGCGI AWSIAEHLAK ETKCFTLFAT
HFHEITKLAE TLSTVKNCHM AAVADADDFT LLYQVRSGVM EKSFGIQVAR LANFPEHVVQ
NAQEVYNEFE DEHVDKQKKE DKALLEKIQV AIQQLSTAGN NVDINVEDLT QLVTQFTKDI
EQLDSDYFKS VLATSEA
